The location of an engineered inter-subunit disulfide bond in factor for inversion stimulation (FIS) affects the denaturation pathway and cooperativity

Biochemistry

Volumn 45, Issue 32, 2006, Pages 9767-9777

  Meinhold, Derrick ^a   Beach, Michael ^b   Shao, Yongping ^c   Osuna, Robert ^c   Colón, Wilfredo ^a  

^a Rensselaer Polytechnic Institute   (United States)

^b STATE UNIVERSITY OF NEW YORK   (United States)

^c SOUTHERN POLYTECHNIC STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPUTER SIMULATION; DIMERS; DNA; MOLECULAR DYNAMICS; PHASE EQUILIBRIA; STABILIZATION;

DENATURATION; DNA BINDING; FACTOR FOR INVERSION STIMULATION (FIS); INTERMOLECULAR DISULFIDE BOND;

PROTEINS;

CYSTEINE; DNA BINDING PROTEIN; FACTOR FOR INVERSION STIMULATION; GUANIDINE; HOMODIMER; MUTANT PROTEIN; PROTEIN SUBUNIT; SERINE; TRYPTOPHAN; TYROSINE; UREA; VALINE;

ACIDITY; AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; CIRCULAR DICHROISM; COOPERATIVE DENATURATION; DISULFIDE BOND; DNA BINDING; GENE MUTATION; MOLECULAR INTERACTION; OXIDATION; PH; PRIORITY JOURNAL; PROTEIN CROSS LINKING; PROTEIN DENATURATION; PROTEIN ENGINEERING; PROTEIN INTERACTION; PROTEIN MODIFICATION; PROTEIN SECONDARY STRUCTURE; PROTEIN STABILITY; PROTEIN TERTIARY STRUCTURE; PROTEIN VARIANT; REDUCTION;

CIRCULAR DICHROISM; CRYSTALLOGRAPHY, X-RAY; CYSTEINE; DISULFIDES; DNA; FACTOR FOR INVERSION STIMULATION PROTEIN; FLUORESCENCE; GUANIDINE; HYDROGEN BONDING; HYDROGEN-ION CONCENTRATION; MODELS, MOLECULAR; MUTATION; OXIDATION-REDUCTION; PROTEIN BINDING; PROTEIN DENATURATION; PROTEIN ENGINEERING; PROTEIN SUBUNITS; SERINE; STRUCTURE-ACTIVITY RELATIONSHIP; UREA; VALINE;

EID: 33747730278     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi060672n     Document Type: Article

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