Equilibrium denaturation studies of the Escherichia coli factor for inversion stimulation: Implications for in vivo function

Protein Science

Volumn 11, Issue 7, 2002, Pages 1671-1680

  Hobart, Sarah A ^a   Ilin, Sergey ^a   Moriarty, Daniel F ^a   Osuna, Robert ^b   Colón, Wilfredo ^a  

^a Research Rensselaer Polytechnic Institute   (United States)

^b STATE UNIVERSITY OF NEW YORK   (United States)

Author keywords

Dimer stability; Dissociation constant; DNA binding; Equilibrium denaturation; Factor for inversion stimulation, FIS; Transcription regulation

Indexed keywords

DNA BINDING PROTEIN; FACTOR FOR INVERSION STIMULATION; UNCLASSIFIED DRUG; UREA; ESCHERICHIA COLI PROTEIN; TYROSINE;

ARTICLE; BACTERIAL GROWTH; CIRCULAR DICHROISM; CONCENTRATION RESPONSE; DISSOCIATION CONSTANT; ESCHERICHIA COLI; FLUORESCENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; PRIORITY JOURNAL; PROTEIN DENATURATION; PROTEIN QUATERNARY STRUCTURE; THERMODYNAMICS; TRANSCRIPTION REGULATION; CHEMISTRY; FLUORESCENCE POLARIZATION; KINETICS; METABOLISM; PHYSIOLOGY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CIRCULAR DICHROISM; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FACTOR FOR INVERSION STIMULATION PROTEIN; FLUORESCENCE; FLUORESCENCE POLARIZATION; KINETICS; PROTEIN DENATURATION; TYROSINE; UREA;

EID: 0036084703     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.5050102     Document Type: Article

References (61)

1. Phage phi29 protein p6 is in a monomer-dimer equilibrium that shifts to higher association states at the millimolar concentrations found in vivo
2. The mechanism of reassembly of immunoglobulin G
3. Efficient excision of phage lambda from the Escherichia coli chromosome requires the Fis protein
4. Dramatic changes in Fis levels upon nutrient upshift in Escherichia coli
5. Identification and characterization of the fis operon in enteric bacteria
6. Folding mechanism of the alpha-subunit of tryptophan synthase, an alpha/beta barrel protein: Global analysis highlights the interconversion of multiple native, intermediate, and unfolded forms through parallel channels
7. FIS-dependent trans-activation of tRNA and rRNA operons of Escherichia coli
8. Fis binding to the recombinational enhancer of the Hin DNA inversion system
9. The ubiquitin-proteasome pathway: On protein death and cell life
10. The regulation of protein function by multisite phosphorylation-A 25 year update
11. Stimulation of DNA inversion by FIS: Evidence for enhancer-independent contacts with the Gin-gix complex
12. Xis and Fis proteins prevent site-specific DNA inversion in lysogens of phage HK022
13. The use of fluorescence methods to monitor unfolding transitions in proteins
14. Effector-induced self-association and conformational changes in the enhancer-binding protein NTRC
15. The Fis protein: It's not just for DNA inversion anymore
16. A model for circular dichroism monitored dimerization and calcium binding in an EF-hand synthetic peptide
17. FIS is a regulator of metabolism in Escherichia coli
18. DNA binding and bending are necessary but not sufficient for Fis-dependent activation of rrnB P1
19. Purification and DNA-binding properties of FIS and Cin, two proteins required for the bacteriophage PI site-specific recombination system, cin
20. A folded monomeric intermediate in the formation of lambda Cro dimer-DNA complexes
21. Host protein requirements for in vitro site-specific DNA-inversion
22. Purification and properties of the Escherichia coli host factor required for inversion of the G segment in bacteriophage Mu
23. The N-terminal part of the E. coli DNA binding protein FIS is essential for stimulating site-specific DNA inversion but is not required for specific DNA binding
24. Three-dimensional structure of the E. coli DNA-binding protein FIS
25. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures
26. Substrate targeting in the ubiquitin system
27. Nitrogen regulatory locus "glnR" of enteric bacteria is composed of cistrons ntrB and ntrC: Identification of their protein products
28. Evolution of new protein function: Recombinational enhancer Fis originated by horizontal gene transfer from the transcriptional regulator NtrC
29. Protein structure analysis using circular dichroism
30. Conformational stability of dimeric proteins: Quantitative studies by equilibrium denaturation
31. Chemical composition of Escherichia coli
32. FIS-dependent trans activation of stable RNA operons of Escherichia coli under various growth conditions
33. The E. coli fis promoter is subject to stringent control and autoregulation
34. Identification of two functional regions in Fis: The N-terminus is required to promote Hin-mediated DNA inversion but not lambda excision
35. Sequence, regulation, and functions of fis in Salmonella typhimurium
36. Measuring the conformational stability of a protein
37. How to measure and predict the molar absorption coefficient of a protein
38. A monomer-dimer equilibrium modulates the interaction of the sunflower homeodomain leucine-zipper protein Hahb-4 with DNA
39. Solution structure of the DNA-binding domain of NtrC with three alanine substitutions
40. Oligomerization of NTRC at the glnA enhancer is required for transcriptional activation
41. Deletion analysis of the fis promoter region in Escherichia coli: Antagonistic effects of integration host factor and Fis
42. Responses of Gram-negative bacteria to certain environmental stressors
43. Association states of the transcription activator protein NtrC from E. coli determined by analytical ultracentrifugation
44. E. coli Fis protein activates ribosomal RNA transcription in vitro and in vivo
45. The transactivation region of the fis protein that controls site-specific DNA inversion contains extended mobile beta-hairpin arms
46. Solvent denaturation
47. The thermodynamic stability of proteins
48. FIS modulates growth phase-dependent topological transitions of DNA in Escherichia coli
49. Characterization of the dimer interface of transcription factor NFkappaB p50 homodimer
50. DNA binding and oligomerization of NtrC studied by fluorescence anisotropy and fluorescence correlation spectroscopy
51. Transcriptional repressor CopR: Amino acids involved in forming the dimeric interface
52. DNA-looping and enhancer activity: Association between DNA-bound NtrC activator and RNA polymerase at the bacterial glnA promoter
53. Cellular factors couple recombination with growth phase: Characterization of a new component in the lambda site-specific recombination pathway
54. Complex behavior in solution of homodimeric SecA
55. Unusual oligomerization required for activity of NtrC, a bacterial enhancer-binding protein
56. Mechanism and evolution of protein dimerization
57. Fis activates the RpoS-dependent stationary-phase expression of proP in Escherichia coli
58. Identification of genes negatively regulated by Fis: Fis and RpoS comodulate growth-phase-dependent gene expression in Escherichia coli
59. The molecular structure of wild-type and a mutant Fis protein: Relationship between mutational changes and recombinational enhancer function or DNA binding
60. Probing the folding mechanism of a leucine zipper peptide by stopped-flow circular dichroism spectroscopy