Differentiation between conformational and autoproteolytic stability of the neutral protease from Bacillus stearothermophilus containing an engineered disulfide bond

European Journal of Biochemistry

Volumn 268, Issue 12, 2001, Pages 3612-3618

  Dürrschmidt, Peter ^a   Mansfeld, Johanna ^a   Ulbrich Hofmann, Renate ^a  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

Author keywords

Autoproteolysis; Disulfide bond; Neutral protease; Stability; Unfolding

Indexed keywords

DISULFIDE; GUANIDINE; NEUTRAL PROTEINASE;

ARTICLE; CATALYSIS; CIRCULAR DICHROISM; CONTROLLED STUDY; DENATURATION; DISULFIDE BOND; ENZYME INACTIVATION; ENZYME INHIBITION; ENZYME METABOLISM; FLUORESCENCE; GEOBACILLUS STEAROTHERMOPHILUS; INCUBATION TIME; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DEGRADATION; PROTEIN FOLDING; PROTEIN STABILITY; QUANTITATIVE ASSAY; TEMPERATURE;

BACILLUS STEAROTHERMOPHILUS; CIRCULAR DICHROISM; DISULFIDES; ENDOPEPTIDASES; ENZYME STABILITY; GUANIDINE; MODELS, MOLECULAR; PROTEASE INHIBITORS; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN ENGINEERING; SPECTROMETRY, FLUORESCENCE;

BACTERIA (MICROORGANISMS); GEOBACILLUS STEAROTHERMOPHILUS; POSIBACTERIA;

EID: 0034819685     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2001.02270.x     Document Type: Article

References (24)

1. Domain unfolding and the stability of thermolysin in guanidine hydrochloride
2. Temperature-gradient gel electrophoresis for analysis and screening of thermostable proteases
3. Conformational stability and catalytic activity of HIV-1 protease are both enhanced at high salt concentration
4. Alteration of specific activity and stability of thermostable neutral protease by site-directed mutagenesis
5. Elucidation of the thermal stability of the neutral proteinase II from Aspergillus oryzae
6. Improvement of thermal stability of subtilisin J by changing the primary autolysis site
7. Early steps in the unfolding of thermolysin-like proteases
8. Protein engineering of subtilisin BPN′: Enhanced stabilization through the introduction of two cysteines to form a disulfide bond
9. Enhancement of the thermostability of subtilisin E by introduction of a disulfide bond engineered on the basis of structural comparison with a thermophilic serine protease
10. Extreme stabilization of a thermolysin-like protease by an engineered disulfide bond
11. Purification and characterization of aqualysin I (a thermophilic alkaline serine protease) produced by Thermus aquaticus YT-1
12. Nucleotide sequence and promoter region for the neutral protease gene from Bacillus stearothermophilus
13. Engineering thermolysin-like proteases whose stability is largely independent of calcium
14. A single calcium binding site is crucial for the calcium-dependent thermal stability of thermolysin-like proteases
15. Prediction and analysis of structure, stability and unfolding of thermolysin-like proteases
16. Structural determinants of the stability of thermolysin-like proteinases
17. Mutational analysis of a surface area that is critical for the thermal stability of thermolysin-like proteases
18. The effect of cavity-filling mutations on the thermostability of Bacillus stearothermophilus neutral protease
19. Quantitative protein precipitation from guanidine hydrochloride-containing solutions by sodium deoxycholate/trichloroacetic acid
20. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
21. A spectrophotometric assay for neutral protease
22. The determination of enzyme inhibitor constants
23. Incorporation of a stabilizing Ca(2+)-binding loop into subtilisin BPN′
24. Thermostability of Bacillus subtilis neutral protease