Enhanced growth of Sf-9 cells to a maximum density of 5.2 x 107 cells per mL and production of β-galactosidase at high cell density by fed batch culture

Biotechnology and Bioengineering

Volumn 68, Issue 4, 2000, Pages 381-388

  Elias, Cynthia B ^a   Zeiser, Arno ^a   Bédard, Charles ^a,b   Kamen, Amine A ^a  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^b TKT Inc   (United States)

Author keywords

galactosidase; Baculovirus expression vector system; Fed batch culture; Online monitoring; Sf 9 cells

Indexed keywords

ANIMAL CELL CULTURE; BIOMASS; BIOREACTORS; BIOSYNTHESIS; CONTINUOUS CELL CULTURE; ENZYME KINETICS; GROWTH KINETICS; PRODUCTIVITY;

CELL DENSITY; FED BATCH CULTURE;

BATCH CELL CULTURE;

ANALYTIC METHOD; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BACTERIUM CULTURE; BIOREACTOR; CELL DENSITY; CELL GROWTH; CONTROLLED STUDY; ENZYME SYNTHESIS; FED BATCH CULTURE; NONHUMAN;

ANIMALS; BETA-GALACTOSIDASE; CELL COUNT; CELL CULTURE TECHNIQUES; CELL DIVISION; CULTURE MEDIA; SPODOPTERA;

ANIMALIA; BACTERIA (MICROORGANISMS); INSECTA; UNIDENTIFIED BACULOVIRUS;

EID: 0034690746     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(20000520)68:4<381::AID-BIT3>3.0.CO;2-D     Document Type: Article

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