On-line monitoring of physiological parameters of insect cell cultures during the growth and infection process

Biotechnology Progress

Volumn 16, Issue 5, 2000, Pages 803-808

  Zeiser, A ^a   Elias, C B ^a   Voyer, R ^a   Jardin, B ^a   Kamen, A A ^a  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BIOVOLUME; METABOLITE CONCENTRATIONS; ON LINE MONITORING; PHYSIOLOGICAL PARAMETERS; PROTEIN PRODUCTION; RELATIVE PERMITTIVITY;

BACULOVIRUS; CARBON DIOXIDE; CELL CULTURE MONITORING; CELL DENSITY; CELL GROWTH; EXPRESSION VECTOR; INSECT CELL CULTURE; LEPIDOPTERA; ONLINE MONITORING; PHYSIOLOGICAL PARAMETER; TRICHOPLUSIA NI; VIRUS INFECTION;

BIOREACTORS; CARBON DIOXIDE; OPTIMIZATION; PERMITTIVITY; PHYSIOLOGY; PROTEINS; VIRUSES;

CELL CULTURE;

ANIMALS; BACULOVIRIDAE; BIOREACTORS; CELL DIVISION; CELL LINE; MOTHS;

INSECTA; LEPIDOPTERA; SPODOPTERA FRUGIPERDA; TRICHOPLUSIA NI; UNIDENTIFIED BACULOVIRUS;

EID: 0034281764     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp000092w     Document Type: Article

References (13)

1. On-line monitoring and control of the cultivation of Spodoptera frugiperda Sf-9 insect cells and β-galactosidase production by Autographa californica virus vector
2. Maximization of recombinant protein yields in the insect cell/baculovirus system by one-time addition of nutrients to high-density batch cultures
3. Insect cell density in bioreactor cultures can be estimated from on-line measurements of optical density
4. 7 cells/mL and improves baculovirus-expressed recombinant protein yields
5. On-line monitoring of respiration in recombinant-baculovirus-infected and uninfected insect cell bioreactor cultures
6. Accuracy of the endpoint assay for virus titration
7. Using oxygen uptake rates to time infections in the baculovirus expression vector system
8. The effect of dissolved oxygen tension and the utility of oxygen uptake rate in insect cell culture
9. DNA distribution and respiratory activity of Spodoptera frugiperda populations infected with wild-type and recombinant Autographa callfornica nuclear polyhedrosis virus
10. Effect of elevated oxygen and glutamine levels on foreign protein production at high cell densities using the insect cell-baculovirus expression system
11. Scale-up of recombinant virus and protein production in stirred tank reactors
12. On-line monitoring of the progress of infection in Sf-9 insect cell cultures using relative permittivity measurements