Selective alteration of the rate-limiting step in cytosolic aldehyde dehydrogenase through random mutagenesis

Biochemistry

Volumn 45, Issue 31, 2006, Pages 9445-9453

  Ho, Kwok Ki ^a   Hurley, Thomas D ^b   Weiner, Henry ^a  

^a PURDUE UNIVERSITY   (United States)

^b INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASSAYS; CATALYST ACTIVITY; ENZYMES; IONS; MUTAGENESIS; NITROGEN COMPOUNDS; SUBSTITUTION REACTIONS;

ALDEHYDE DEHYDROGENASE; CATALYTIC ACTION; CYTOSOLIC ALDEHYDE; RATE-LIMITING;

ALDEHYDES;

4 NITROPHENYLACETIC ACID; ACETIC ACID DERIVATIVE; ALDEHYDE DEHYDROGENASE; ALDEHYDE DEHYDROGENASE ISOENZYME 1; CHLOROACETALDEHYDE; MAGNESIUM ION; NICOTINAMIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; SERINE; THREONINE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; ASSAY; CATALYSIS; CYTOSOL; DEACYLATION; DISSOCIATION; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME INHIBITION; ENZYME REGULATION; FILTER; HUMAN; HYDROLYSIS; MUTAGENESIS; NONHUMAN; OXIDATION; POINT MUTATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; REACTION ANALYSIS; REACTION TIME; SATURATION MUTAGENESIS; SCREENING; SEQUENCE ANALYSIS; STRUCTURE ANALYSIS;

ALDEHYDE DEHYDROGENASE; ANIMALS; CATALYSIS; CATIONS, DIVALENT; COMPUTATIONAL BIOLOGY; CYTOSOL; ENZYME ACTIVATION; ESTERASES; HUMANS; HYDROLYSIS; ISOENZYMES; MAGNESIUM; MODELS, MOLECULAR; MUTAGENESIS; NAD; NITROPHENOLS; POINT MUTATION; PROTEIN CONFORMATION; SHEEP;

OVIS ARIES;

EID: 33747156202     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi060718c     Document Type: Article

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