The Mitomycin C (MMC)-binding Protein from MMC-producing Microorganisms Protects from the Lethal Effect of Bleomycin: Crystallographic Analysis to Elucidate the Binding Mode of the Antibiotic to the Protein

Journal of Molecular Biology

Volumn 360, Issue 2, 2006, Pages 398-408

  Danshiitsoodol, Narandalai ^a   de Pinho, Catherine Azzariti ^a   Matoba, Yasuyuki ^a   Kumagai, Takanori ^a   Sugiyama, Masanori ^a,b  

^a HIROSHIMA UNIVERSITY   (Japan)

^b HIROSHIMA UNIVERSITY   (Japan)

Author keywords

anti cooperativity; antibiotic resistance; bleomycin A2; crystal structure; mitomycin C binding protein

Indexed keywords

ANTIBIOTIC AGENT; BINDING PROTEIN; BLEOMYCIN; COPPER; MITOMYCIN C; THIAZOLE DERIVATIVE; BACTERIAL PROTEIN; MITOMYCIN;

ARTICLE; BINDING AFFINITY; COMPLEX FORMATION; CRYSTAL STRUCTURE; CRYSTALLOGRAPHY; DRUG BINDING; DRUG EFFECT; ESCHERICHIA COLI; METAL BINDING; MICROORGANISM; NONHUMAN; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; STREPTOMYCES; X RAY CRYSTALLOGRAPHY; ANTIBIOTIC RESISTANCE; BACTERIAL GENE; BINDING SITE; BIOSYNTHESIS; CHEMICAL STRUCTURE; CHEMISTRY; ELECTRON; FLUORESCENCE; GENETICS; METABOLISM; PROTEIN BINDING;

ESCHERICHIA COLI; STREPTOMYCES VERTICILLUS;

BACTERIAL PROTEINS; BINDING SITES; BLEOMYCIN; COPPER; CRYSTALLOGRAPHY, X-RAY; DRUG RESISTANCE, MICROBIAL; ELECTRONS; FLUORESCENCE; GENES, BACTERIAL; MITOMYCIN; MODELS, MOLECULAR; PROTEIN BINDING; STREPTOMYCES;

EID: 33746721635     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2006.05.017     Document Type: Article

References (28)

1. Fong D.H., and Berghuis A.M. Substrate promiscuity of an aminoglycoside antibiotic resistance enzyme via target mimicry. EMBO J. 21 (2002) 2323-2331
2. Choudhuri B.S., Bhakta S., Barik R., Basu J., Kundu M., and Chakrabarti P. Overexpression and functional characterization of an ABC (ATP-binding cassette) transporter encoded by the genes drrA and drrB of Mycobacterium tuberculosis. Biochem. J. 367 (2002) 279-285
3. Perreten V., Schwarz F.V., Teuber M., and Levy S.B. Mdt(A), a new efflux protein conferring multiple antibiotic resistance in Lactococcus lactis and Escherichia coli. Antimicrob. Agents Chemother. 45 (2001) 1109-1114
4. Milner J.L., Stohl E.A., and Handelsman J. Zwittermicin A resistance gene from Bacillus cereus. J. Bacteriol. 178 (1996) 4266-4272
5. Lazaro E., Sanz E., Remacha M., and Ballesta J.P.G. Characterization of sparsomycin resistance in Streptomyces sparsogenes. Antimicrob. Agents Chemother. 46 (2002) 2914-2919
6. Umezawa H. Chemistry and mechanism of action of bleomycin. Fed. Proc. Fed. Am. Soc. Expt. Biol. 33 (1974) 2296-2302
7. Ehrenfeld G.M., Shipley J.B., Heimbrook D.C., Sugiyama H., Long E.C., van Broom J.H., et al. Copper-dependent cleavage of DNA by bleomycin. Biochemistry 26 (1987) 931-942
8. Sugiyama M., Kumagai T., Hayashida M., Maruyama M., and Matoba Y. The 1.6-Å crystal structure of the copper(II)-bound bleomycin complexed with the bleomycin-binding protein from bleomycin-producing Streptomyces verticillus. J. Biol. Chem. 277 (2002) 2311-2329
9. Sugiyama M., Thompson C.J., Kumagai T., Suzuki K., Deblaere R., Villarroel R., and Davies J. Characterisation by molecular cloning of two genes from Streptomyces verticillus encoding resistance to bleomycin gene. Gene 151 (1994) 11-16
10. Sheldon P.J., Johnson D.A., August P.R., Liu H.W., and Sherman D.H. Characterization of a mitomycin-binding drug resistance mechanism from the producing organism, Streptomyces lavendulae. J. Bacteriol. 179 (1997) 1796-1804
11. He M., Sheldon P.J., and Sherman D.H. Characterization of a quinone reductase activity for the mitomycin C binding protein (MRD): Functional switching from a drug-activating enzyme to a drug-binding protein. Proc. Natl Acad. Sci. USA 98 (2001) 926-9311
12. Martin T.W., Dauter Z., Devedjiev Y., Sheffield P., Jelen F., He M., et al. Molecular basis of mitomycin C resistance in Streptomyces: structure and function of the MRD protein. Structure 10 (2002) 933-942
13. Maruyama M., Kumagai T., Matoba Y., Hayashida M., Fujii T., Hata Y., and Sugiyama M. Crystal structures of the transposon Tn5-carried bleomycin resistance determinant uncomplexed and complexed with bleomycin. J. Biol. Chem. 276 (2001) 9992-9999
14. +. J. Am. Chem. Soc. 124 (2002) 11001-11003
15. He M.M., Clugston S.L., Honek J.F., and Matthews B.W. Determination of the structure of Escherichia coli glyoxalase I suggests a structural basis for differential metal activation. Biochemistry 39 (2000) 8719-8728
16. Xu R.X., Nettesheim D., Otvos J.D., and Petering D.H. NMR determination of the structures of peroxycobalt(III) bleomycin and cobalt(III) bleomycin, products of the aerobic oxidation of cobalt(II) bleomycin by dioxygen. Biochemistry 33 (1994) 907-916
17. 2 green: Its detailed structural characterization by NMR and molecular modeling and its sequence-specific interaction with DNA oligonucleotides. J. Am. Chem. Soc. 118 (1996) 1268-1280
18. 2 green complexed with d(CCAGGCCTGG). J. Am. Chem. Soc. 118 (1996) 1281-1294
19. Sugiyama M., Kumagai T., Matuo H., Bhuiyan M.Z.A., Ueda K., Mochizuki H., et al. Overproduction of the bleomycin-binding proteins from bleomycin-producing Streptomyces verticillus and a methicillin-resistant Staphylococcus aureus in Escherichia coli and their immunological characterization. FEBS Letters 362 (1995) 80-84
20. Collaborative Computational Project Number 4. CCP4: programs for protein crystallography. Acta Crystallog. sect. D 50 (1994) 760-763
21. Brünger A.T., Adams P.D., Clore G.M., DeLano W.L., Gros P., Grosse-Kunstleve R.W., et al. Crystallography and NMR system: A new software suite for macromolecular structure determination. Acta Crystallog. sect. D 54 (1998) 905-921
22. Brünger A.T. The Free R value: a novel statistical quantity for assessing the accuracy of crystal structures. Nature 355 (1992) 472-474
23. McRee D.E. XtalView: a visual protein crystallographic software system for XII/Xview. J. Mol. Graph. 10 (1992) 44-46
24. Kraulis P.J. MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures. J. Appl. Crystallog. 24 (1991) 945-950
25. Bacon D.J., and Anderson W.F. A fast algorithm for rendering space-filling molecular pictures. J. Mol. Graph. 6 (1988) 219-220
26. Merritt E.A., and Bacon D.J. Raster3D photorealistic molecular graphics. Methods Enzymol. 277 (1997) 505-552
27. Nicholls A., Sharp K.A., and Honig B. Protein folding and association- insights from the interfacial and thermodynamic properties of hydrocarbons. Proteins: Struct. Funct. Genet. 11 (1991) 281-2963
28. Laskowski R.A., McArthur M.W., Moss D.S., and Thornton J.M. PROCHECK: a program to check the stereochemical quality of protein structure. J. Appl. Crystallog. 26 (1993) 283-291