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1
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0035354575
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Small is mighty: EmrE, a multidrug transporter as an experimental paradigm
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Schuldiner S., Granot D., Mordoch S.S., Ninio S., Rotem D., Soskin M., Tate C.G., and Yerushalmi H. Small is mighty: EmrE, a multidrug transporter as an experimental paradigm. News Physiol Sci 16 (2001) 130-134
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(2001)
News Physiol Sci
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Schuldiner, S.1
Granot, D.2
Mordoch, S.S.3
Ninio, S.4
Rotem, D.5
Soskin, M.6
Tate, C.G.7
Yerushalmi, H.8
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2
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0035088487
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Precious things come in little packages
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Schuldiner S., Granot D., Steiner S., Ninio S., Rotem D., Soskin M., and Yerushalmi H. Precious things come in little packages. J Mol Microbiol Biotechnol 3 (2001) 155-162
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(2001)
J Mol Microbiol Biotechnol
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Schuldiner, S.1
Granot, D.2
Steiner, S.3
Ninio, S.4
Rotem, D.5
Soskin, M.6
Yerushalmi, H.7
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3
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0029856981
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Negative dominance studies demonstrate the oligomeric structure of EmrE, a multidrug antiporter from Escherichia coli
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Yerushalmi H., Lebendiker M., and Schuldiner S. Negative dominance studies demonstrate the oligomeric structure of EmrE, a multidrug antiporter from Escherichia coli. J Biol Chem 271 (1996) 31044-31048
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(1996)
J Biol Chem
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Yerushalmi, H.1
Lebendiker, M.2
Schuldiner, S.3
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4
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0035930510
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In vitro monomer swapping in EmrE, a multidrug transporter from Escherichia coli, reveals that the oligomer is the functional unit
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Rotem D., Sal-man N., and Schuldiner S. In vitro monomer swapping in EmrE, a multidrug transporter from Escherichia coli, reveals that the oligomer is the functional unit. J Biol Chem 276 (2001) 48243-48249
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(2001)
J Biol Chem
, vol.276
, pp. 48243-48249
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Rotem, D.1
Sal-man, N.2
Schuldiner, S.3
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5
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0037126033
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Crosslinking of membrane-embedded cysteines reveals contact points in the EmrE oligomer
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Soskine M., Steiner-Mordoch S., and Schuldiner S. Crosslinking of membrane-embedded cysteines reveals contact points in the EmrE oligomer. Proc Natl Acad Sci USA 99 (2002) 12043-12048
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(2002)
Proc Natl Acad Sci USA
, vol.99
, pp. 12043-12048
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Soskine, M.1
Steiner-Mordoch, S.2
Schuldiner, S.3
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6
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1242319564
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In vitro synthesis of fully functional EmrE, a multidrug transporter, and study of its oligomeric state
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Elbaz Y., Steiner-Mordoch S., Danieli T., and Schuldiner S. In vitro synthesis of fully functional EmrE, a multidrug transporter, and study of its oligomeric state. Proc Natl Acad Sci USA 101 (2004) 1519-1524
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(2004)
Proc Natl Acad Sci USA
, vol.101
, pp. 1519-1524
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Elbaz, Y.1
Steiner-Mordoch, S.2
Danieli, T.3
Schuldiner, S.4
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8
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1942532338
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New insights into the structure and oligomeric state of the bacterial multidrug transporter EmrE: an unusual asymmetric homo-dimer
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Ubarretxena-Belandia I., and Tate C.G. New insights into the structure and oligomeric state of the bacterial multidrug transporter EmrE: an unusual asymmetric homo-dimer. FEBS Lett 564 (2004) 234-238
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(2004)
FEBS Lett
, vol.564
, pp. 234-238
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Ubarretxena-Belandia, I.1
Tate, C.G.2
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9
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0038146916
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Characterization of an archaeal multidrug transporter with a unique amino acid composition
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Ninio S., and Schuldiner S. Characterization of an archaeal multidrug transporter with a unique amino acid composition. J Biol Chem 278 (2003) 12000-12005
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(2003)
J Biol Chem
, vol.278
, pp. 12000-12005
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Ninio, S.1
Schuldiner, S.2
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10
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0035930531
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Functional analysis of novel multidrug transporters from human pathogens
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Ninio S., Rotem D., and Schuldiner S. Functional analysis of novel multidrug transporters from human pathogens. J Biol Chem 276 (2001) 48250-48256
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(2001)
J Biol Chem
, vol.276
, pp. 48250-48256
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Ninio, S.1
Rotem, D.2
Schuldiner, S.3
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11
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0034087478
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A broad-specificity multidrug efflux pump requiring a pair of homologous SMR-type proteins
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Jack D.L., Storms M.L., Tchieu J.H., Paulsen I.T., and Saier Jr. M.H. A broad-specificity multidrug efflux pump requiring a pair of homologous SMR-type proteins. J Bacteriol 182 (2000) 2311-2313
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J Bacteriol
, vol.182
, pp. 2311-2313
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Jack, D.L.1
Storms, M.L.2
Tchieu, J.H.3
Paulsen, I.T.4
Saier Jr., M.H.5
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12
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0034029137
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A two-component multidrug efflux pump, EbrAB, in Bacillus subtilis
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Masaoka Y., Ueno Y., Morita Y., Kuroda T., Mizushima T., and Tsuchiya T. A two-component multidrug efflux pump, EbrAB, in Bacillus subtilis. J Bacteriol 182 (2000) 2307-2310
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J Bacteriol
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, pp. 2307-2310
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Masaoka, Y.1
Ueno, Y.2
Morita, Y.3
Kuroda, T.4
Mizushima, T.5
Tsuchiya, T.6
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14
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0029788690
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Identification of residues in the translocation pathway of EmrE, a multidrug antiporter from Escherichia coli
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Lebendiker M., and Schuldiner S. Identification of residues in the translocation pathway of EmrE, a multidrug antiporter from Escherichia coli. J Biol Chem 271 (1996) 21193-21199
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(1996)
J Biol Chem
, vol.271
, pp. 21193-21199
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Lebendiker, M.1
Schuldiner, S.2
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15
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0033516475
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+-coupled multidrug transporter from Escherichia coli, reveals a hydrophobic pathway for solutes
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+-coupled multidrug transporter from Escherichia coli, reveals a hydrophobic pathway for solutes. J Biol Chem 274 (1999) 19480-19486
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(1999)
J Biol Chem
, vol.274
, pp. 19480-19486
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Mordoch, S.S.1
Granot, D.2
Lebendiker, M.3
Schuldiner, S.4
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16
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0034677201
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A membrane-embedded glutamate is required for ligand binding to the multidrug transporter EmrE
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Muth T.R., and Schuldiner S. A membrane-embedded glutamate is required for ligand binding to the multidrug transporter EmrE. EMBO J 19 (2000) 234-240
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(2000)
EMBO J
, vol.19
, pp. 234-240
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Muth, T.R.1
Schuldiner, S.2
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17
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0034051663
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An essential glutamyl residue in EmrE, a multidrug antiporter from Escherichia coli
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Yerushalmi H., and Schuldiner S. An essential glutamyl residue in EmrE, a multidrug antiporter from Escherichia coli. J Biol Chem 275 (2000) 5264-5269
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(2000)
J Biol Chem
, vol.275
, pp. 5264-5269
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Yerushalmi, H.1
Schuldiner, S.2
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18
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0035918237
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A single carboxyl mutant of the multidrug transporter EmrE is fully functional
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Yerushalmi H., Mordoch S.S., and Schuldiner S. A single carboxyl mutant of the multidrug transporter EmrE is fully functional. J Biol Chem 276 (2001) 12744-12748
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(2001)
J Biol Chem
, vol.276
, pp. 12744-12748
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Yerushalmi, H.1
Mordoch, S.S.2
Schuldiner, S.3
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19
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0037507278
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An amino acid cluster around the essential Glu-14 is part of the substrate- and proton-binding domain of EmrE, a multidrug transporter from Escherichia coli
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Gutman N., Steiner-Mordoch S., and Schuldiner S. An amino acid cluster around the essential Glu-14 is part of the substrate- and proton-binding domain of EmrE, a multidrug transporter from Escherichia coli. J Biol Chem 278 (2003) 16082-16087
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(2003)
J Biol Chem
, vol.278
, pp. 16082-16087
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Gutman, N.1
Steiner-Mordoch, S.2
Schuldiner, S.3
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20
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25444519556
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Exploring the binding domain of EmrE, the smallest multidrug transporter
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Mutagenesis and labelling strategies combined to define residues in EmrE that are essential for the binding of substrates.
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Sharoni M., Steiner-Mordoch S., and Schuldiner S. Exploring the binding domain of EmrE, the smallest multidrug transporter. J Biol Chem 280 (2005) 32849-32855. Mutagenesis and labelling strategies combined to define residues in EmrE that are essential for the binding of substrates.
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(2005)
J Biol Chem
, vol.280
, pp. 32849-32855
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Sharoni, M.1
Steiner-Mordoch, S.2
Schuldiner, S.3
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21
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0034610304
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A model for coupling of H(+) and substrate fluxes based on "time-sharing" of a common binding site
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Yerushalmi H., and Schuldiner S. A model for coupling of H(+) and substrate fluxes based on "time-sharing" of a common binding site. Biochemistry 39 (2000) 14711-14719
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(2000)
Biochemistry
, vol.39
, pp. 14711-14719
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Yerushalmi, H.1
Schuldiner, S.2
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22
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0034733974
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A common binding site for substrates and protons in EmrE, an ion-coupled multidrug transporter
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Yerushalmi H., and Schuldiner S. A common binding site for substrates and protons in EmrE, an ion-coupled multidrug transporter. FEBS Lett 476 (2000) 93-97
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(2000)
FEBS Lett
, vol.476
, pp. 93-97
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Yerushalmi, H.1
Schuldiner, S.2
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23
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14844322310
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Exploring the role of a unique carboxyl residue in EmrE by mass spectrometry
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Weinglass A.B., Soskine M., Vazquez-Ibar J.L., Whitelegge J.P., Faull K.F., Kaback H.R., and Schuldiner S. Exploring the role of a unique carboxyl residue in EmrE by mass spectrometry. J Biol Chem 280 (2005) 7487-7492
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(2005)
J Biol Chem
, vol.280
, pp. 7487-7492
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Weinglass, A.B.1
Soskine, M.2
Vazquez-Ibar, J.L.3
Whitelegge, J.P.4
Faull, K.F.5
Kaback, H.R.6
Schuldiner, S.7
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24
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10344220028
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EmrE, a multidrug transporter from Escherichia coli, transports monovalent and divalent substrates with the same stoichiometry
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An elegant demonstration that EmrE imports two protons for every hydrophobic cation exported. This paper shows that transport of a drug substrate bearing a single positive charge is electrogenic and transport of a substrate with two positive charges is electroneutral.
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Rotem D., and Schuldiner S. EmrE, a multidrug transporter from Escherichia coli, transports monovalent and divalent substrates with the same stoichiometry. J Biol Chem 279 (2004) 48787-48793. An elegant demonstration that EmrE imports two protons for every hydrophobic cation exported. This paper shows that transport of a drug substrate bearing a single positive charge is electrogenic and transport of a substrate with two positive charges is electroneutral.
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(2004)
J Biol Chem
, vol.279
, pp. 48787-48793
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Rotem, D.1
Schuldiner, S.2
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25
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0035863057
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The projection structure of EmrE, a proton-linked multidrug transporter from Escherichia coli, at 7 Å resolution
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Tate C.G., Kunji E.R., Lebendiker M., and Schuldiner S. The projection structure of EmrE, a proton-linked multidrug transporter from Escherichia coli, at 7 Å resolution. EMBO J 20 (2001) 77-81
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(2001)
EMBO J
, vol.20
, pp. 77-81
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Tate, C.G.1
Kunji, E.R.2
Lebendiker, M.3
Schuldiner, S.4
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26
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0041735000
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Conformational changes in the multidrug transporter EmrE associated with substrate binding
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Tate C.G., Ubarretxena-Belandia I., and Baldwin J.M. Conformational changes in the multidrug transporter EmrE associated with substrate binding. J Mol Biol 332 (2003) 229-242
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(2003)
J Mol Biol
, vol.332
, pp. 229-242
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Tate, C.G.1
Ubarretxena-Belandia, I.2
Baldwin, J.M.3
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29
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21644448221
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Crystallography of the integral membrane protein EmrE from Escherichia coli
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Ma C., and Chang G. Crystallography of the integral membrane protein EmrE from Escherichia coli. Acta Crystallogr D Biol Crystallogr 60 (2004) 2399-2402
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(2004)
Acta Crystallogr D Biol Crystallogr
, vol.60
, pp. 2399-2402
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Ma, C.1
Chang, G.2
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31
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11144305210
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Organic solvent extracted EmrE solubilized in dodecyl maltoside is monomeric and binds drug ligand
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Winstone T.L., Jidenko M., le Maire M., Ebel C., Duncalf K.A., and Turner R.J. Organic solvent extracted EmrE solubilized in dodecyl maltoside is monomeric and binds drug ligand. Biochem Biophys Res Commun 327 (2005) 437-445
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(2005)
Biochem Biophys Res Commun
, vol.327
, pp. 437-445
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Winstone, T.L.1
Jidenko, M.2
le Maire, M.3
Ebel, C.4
Duncalf, K.A.5
Turner, R.J.6
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32
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0038521734
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Structure of the substrate binding pocket of the multidrug transporter EmrE: site-directed spin labeling of transmembrane segment 1
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Koteiche H.A., Reeves M.D., and McHaourab H.S. Structure of the substrate binding pocket of the multidrug transporter EmrE: site-directed spin labeling of transmembrane segment 1. Biochemistry 42 (2003) 6099-6105
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(2003)
Biochemistry
, vol.42
, pp. 6099-6105
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Koteiche, H.A.1
Reeves, M.D.2
McHaourab, H.S.3
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33
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19744376674
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Global topology analysis of the Escherichia coli inner membrane proteome
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Daley D.O., Rapp M., Granseth E., Melen K., Drew D., and von Heijne G. Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 (2005) 1321-1323
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(2005)
Science
, vol.308
, pp. 1321-1323
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Daley, D.O.1
Rapp, M.2
Granseth, E.3
Melen, K.4
Drew, D.5
von Heijne, G.6
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34
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0000651660
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The distribution of positively charged residues in bacterial inner membrane proteins correlates with the trans-membrane topology
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Heijne G.V. The distribution of positively charged residues in bacterial inner membrane proteins correlates with the trans-membrane topology. EMBO J 5 (1986) 3021-3027
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EMBO J
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Heijne, G.V.1
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1642299702
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The membrane topology of EmrE - a small multidrug transporter from Escherichia coli
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Ninio S., Elbaz Y., and Schuldiner S. The membrane topology of EmrE - a small multidrug transporter from Escherichia coli. FEBS Lett 562 (2004) 193-196
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(2004)
FEBS Lett
, vol.562
, pp. 193-196
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Ninio, S.1
Elbaz, Y.2
Schuldiner, S.3
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37
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27344442574
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+ channel and its dependence on the lipid membrane
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vAP. It appears that the detergent micelle is a poor mimic of the lipid bilayer and that lipids are essential for maintaining the structure of the voltage sensor domain.
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vAP. It appears that the detergent micelle is a poor mimic of the lipid bilayer and that lipids are essential for maintaining the structure of the voltage sensor domain.
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(2005)
Proc Natl Acad Sci USA
, vol.102
, pp. 15441-15446
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Lee, S.Y.1
Lee, A.2
Chen, J.3
MacKinnon, R.4
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