Structural biology: X-ray structure of the EmrE multidrug transporter in complex with a substrate

Science

Volumn 310, Issue 5756, 2005, Pages 1950-1953

  Pornillos, Owen ^a   Chen, Yen Ju ^a   Chen, Andy P ^a   Chang, Geoffrey ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL MEMBRANES; CONFORMATIONS; DIMERS; DRUG PRODUCTS; ESCHERICHIA COLI; POLYPEPTIDES;

CONFORMATIONAL STATE; STRUCTURAL BIOLOGY; X-RAY STRUCTURE;

CRYSTAL STRUCTURE;

GLYCOPROTEIN P; PROTEIN EMRE; TETRAPHENYLPHOSPHONIUM; UNCLASSIFIED DRUG; ANTIPORTER; CATION; EMRE PROTEIN, E COLI; ESCHERICHIA COLI PROTEIN; MEMBRANE PROTEIN; ORGANOPHOSPHORUS COMPOUND;

BIOLOGY;

ARTICLE; CRYSTAL STRUCTURE; DIMERIZATION; ENZYME SUBSTRATE; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; CHEMISTRY; METABOLISM; MULTIDRUG RESISTANCE; TRANSPORT AT THE CELLULAR LEVEL; X RAY CRYSTALLOGRAPHY;

ESCHERICHIA COLI;

ANTIPORTERS; BIOLOGICAL TRANSPORT; CRYSTALLOGRAPHY, X-RAY; DRUG RESISTANCE, MULTIPLE, BACTERIAL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MEMBRANE PROTEINS; ONIUM COMPOUNDS; ORGANOPHOSPHORUS COMPOUNDS; PROTEIN CONFORMATION;

EID: 29344450131     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1119776     Document Type: Article

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33. We thank C. Ma for invaluable contributions to EmrE-TPP crystallization; T. Kudlicki and J. Fletcher (Invitrogen) for in vitro translation reagents; S. Lieu for general lab support; and the staff at Stanford Synchrotron Radiation Laboratory, Advanced Light Source, and Advanced Photon Source for assistance with data collection. We thank C.G. Tate (MRC Laboratory of Molecular Biology) for providing the EM map of EmrE-TPP, and S. H. White, P. E. Wright, H. J. Dyson, R. A. Milligan, C. L. Reyes, and Y. Yin for critical reading of the manuscript. This study was supported by grants from the NIH (GM67644 and GM073197) and NASA (NAG8-1834) to G.C. O.P. is supported by an NIH postdoctoral fellowship. Coordinates have been deposited in the Protein Data Bank (PDB code: 2f2m).