Organic solvent extracted EmrE solubilized in dodecyl maltoside is monomeric and binds drug ligand

Biochemical and Biophysical Research Communications

Volumn 327, Issue 2, 2005, Pages 437-445

  Winstone, Tara L ^a   Jidenko, Marie ^b   Maire, Marc Le ^b   Ebel, Christine ^c   Duncalf, Karen A ^a   Turner, Raymond J ^a  

^a UNIVERSITY OF CALGARY   (Canada)

^b CEA SACLAY   (France)

^c INSTITUT DE BIOLOGIE STRUCTURALE   (France)

Author keywords

Chloroform:methanol; Dodecyl maltoside; EmrE; Membrane proteins; Multimers; SEC HPLC; Ultracentrifugation

Indexed keywords

CHLOROFORM; DETERGENT; ETHIDIUM MULTIDRUG RESISTANCE PROTEIN; ISOPROTEIN; METHANOL; MULTIDRUG RESISTANCE PROTEIN; N DODECYL BETA DEXTRO MALTOPYRANOSIDE; ORGANIC SOLVENT; UNCLASSIFIED DRUG;

ARTICLE; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIGAND BINDING; PHYSICAL CHEMISTRY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN INTERACTION; PROTEIN MODIFICATION; PROTEIN PROCESSING; PROTEIN PURIFICATION; PROTEOMICS; SEDIMENTATION RATE; SOLVENT EXTRACTION;

ANTIPORTERS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DETERGENTS; ESCHERICHIA COLI PROTEINS; ETHIDIUM; GLUCOSIDES; LIGANDS; MEMBRANE PROTEINS; SOLUBILITY; SOLVENTS; SPECTROMETRY, FLUORESCENCE; ULTRACENTRIFUGATION;

ESCHERICHIA COLI;

EID: 11144305210     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2004.11.164     Document Type: Article

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