Back on track - On the role of the microtubule for kinesin motility and cellular function

Journal of Muscle Research and Cell Motility

Volumn 27, Issue 2, 2006, Pages 161-171

  Lakämper, Stefan ^a   Meyhöfer, Edgar ^b  

^a VU UNIVERSITY AMSTERDAM   (Netherlands)

^b UNIVERSITY OF MICHIGAN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE DIPHOSPHATE; KINESIN; MICROTUBULE PROTEIN;

ACETYLATION; ALLOSTERISM; AMINO TERMINAL SEQUENCE; CARBOXY TERMINAL SEQUENCE; CELL FUNCTION; CELL INTERACTION; CELL MOTILITY; CELL ULTRASTRUCTURE; CONFORMATIONAL TRANSITION; GLYCATION; MICROTUBULE; MICROTUBULE ASSEMBLY; MOLECULAR INTERACTION; NONHUMAN; NUCLEOTIDE BINDING SITE; PALMITOYLATION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN CROSS LINKING; PROTEIN FAMILY; PROTEIN HYDROLYSIS; PROTEIN PHOSPHORYLATION; PROTEIN PROCESSING; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; PROTEIN SYNTHESIS REGULATION; REVIEW; WESTERN BLOTTING;

ANIMALS; BIOLOGICAL TRANSPORT; CELL MOVEMENT; CILIA; CYTOSKELETON; FLAGELLA; HUMANS; KINESIN; MICROTUBULES; PROTEIN PROCESSING, POST-TRANSLATIONAL;

EUKARYOTA;

EID: 33746102907     PISSN: 01424319     EISSN: 15732657     Source Type: Journal    
DOI: 10.1007/s10974-005-9052-3     Document Type: Review

References (120)

1. Alonso MC, Vanderkerckhove J and Cross RA (1998) Proteolytic mapping of kinesin/ncd-microtubule interface: nucleotide-dependent conformational changes in the loops L8 and L12. EMBO J 17: 945-951.
2. Arce CA, Barra HS, Rodriguez JA and Caputto R (1975) Tentative identification of the amino acid that binds tyrosine as a single unit into a soluble brain protein. FEBS Lett 50: 5-7.
3. Argarana CE, Barra HS and Caputto R (1978) Release of [14C]tyrosine from tubulinyl-[14C]tyrosine by brain extract. Separation of a carboxypeptidase from tubulin-tyrosine ligase. Mol Cell Biochem 19: 17-21.
4. Argarana CE, Barra HS and Caputto R (1980) Tubulinyl-tyrosine carboxypeptidase from chicken brain: properties and partial purification. J Neurochem 34: 114-118.
5. Asbury CL, Fehr AN and Block SM (2003) Kinesin moves by an asymmetric hand-over-hand mechanism. Science 302: 2130-2134.
6. Bathe F, Hahlen K, Dombi R, Driller L, Schliwa M and Woehlke G (2005) The complex interplay between the neck and hinge domains in kinesin-1 dimerization and motor activity. Mol Biol Cell 16: 3529-3537.
7. Bhattacharyya B, Sackett DL and Wolff J (1985) Tubulin, hybrid dimers, and tubulin S. Stepwise charge reduction and polymerization. J Biol Chem 260: 10208-10216.
8. Bobinnec Y, Moudjou M, Fouquet JP, Desbruyeres E, Edde B and Bornens M (1998) Glutamylation of centriole and cytoplasmic tubulin in proliferating non-neuronal cells. Cell Motil Cytoskeleton 39: 223-232.
9. Bonnet C, Boucher D, Lazereg S, Pedrotti B, Islam K, Denoulet P and Larcher JC (2001) Differential binding regulation of microtubule-associated proteins MAP1A, MAP1B, and MAP2 by tubulin polyglutamylation. J Biol Chem 276: 12839-12848.
10. Boucher D, Larcher JC, Gros F and Denoulet P (1994) Polyglutamylation of tubulin as a progressive regulator of in vitro interactions between the microtubule-associated protein Tau and tubulin. Biochemistry 33: 12471-12477.
11. Brady ST (1985) A novel brain ATPase with properties expected for the fast axonal transport motor. Nature 317: 73-75.
12. Bre MH, Redeker V, Quibell M, Darmanaden-Delorme J, Bressac C, Cosson J, Huitorel P, Schmitter JM, Rossler J, Johnson T and others (1996) Axonemal tubulin polyglycylation probed with two monoclonal antibodies: widespread evolutionary distribution, appearance during spermatozoan maturation and possible function in motility. J Cell Sci 109: 727-738.
13. Bre MH, Redeker V, Vinh J, Rossier J and Levilliers N (1998) Tubulin polyglycylation: differential posttranslational modification of dynamic cytoplasmic and stable axonemal microtubules in paramecium. Mol Biol Cell 9: 2655-2665.
14. Brown JM, Hardin C and Gaertig J (1999) Rotokinesis, a novel phenomenon of cell locomotion-assisted cytokinesis in the ciliate Tetrahymena thermophila. Cell Biol Int 23: 841-848.
15. Carter NJ and Cross RA (2005) Mechanics of the kinesin step. Nature 435: 308-312.
16. Chang W, Webster DR, Salam AA, Gruber D, Prasad A, Eiserich JP and Bulinski JC (2002) Alteration of the C-terminal amino acid of tubulin specifically inhibits myogenic differentiation. J Biol Chem 277: 30690-30698.
17. Coy DL, Hancock WO, Wagenbach M and Howard J (1999) Kinesin's tail domain is an inhibitory regulator of the motor domain. Nat Cell Biol 1: 288-292.
18. de Cuevas M, Tao T and Goldstein LS (1992) Evidence that the stalk of Drosophila kinesin heavy chain is an alpha-helical coiled coil. J Cell Biol 116: 957-965.
19. Duan J and Gorovsky MA (2002) Both carboxy-terminal tails of alpha- and beta-tubulin are essential, but either one will suffice. Curr Biol 12: 313-316.
20. Ebneth A, Godemann R, Stamer K, Illenberger S, Trinczek B and Mandelkow E (1998) Overexpression of tau protein inhibits kinesin-dependent trafficking of vesicles, mitochondria, and endoplasmic reticulum: implications for Alzheimer's disease. J Cell Biol 143: 777-794.
21. Eiserich JP, Estevez AG, Bamberg TV, Ye YZ, Chumley PH, Beckman JS and Freeman BA (1999) Microtubule dysfunction by posttranslational nitrotyrosination of alpha-tubulin: a nitric oxide-dependent mechanism of cellular injury. Proc Natl Acad Sci USA 96: 6365-6370.
22. Ersfeld K, Wehland J, Plessmann U, Dodemont H, Gerke V and Weber K (1993) Characterization of the tubulin-tyrosine ligase. J Cell Biol 120: 725-732.
23. Gaertig J, Cruz MA, Bowen J, Gu L, Pennock DG and Gorovsky MA (1995) Acetylation of lysine 40 in alpha-tubulin is not essential in Tetrahymena thermophila. J Cell Biol 129: 1301-1310.
24. Gaertig J, Thatcher TH, McGrath KE, Callahan RC and Gorovsky MA (1993) Perspectives on tubulin isotype function and evolution based on the observation that Tetrahymena thermophila microtubules contain a single alpha- and beta-tubulin. Cell Motil Cytoskeleton 25: 243-253.
25. Gagnon C, White D, Cosson J, Huitorel P, Edde B, Desbruyeres E, Paturle-Lafanechere L, Multigner L, Job D and Cibert C (1996) The polyglutamylated lateral chain of alpha-tubulin plays a key role in flagellar motility. J Cell Sci 109: 1545-1553.
26. Gilbert SP, Moyer ML and Johnson KA (1998) Alternating site mechanism of the kinesin ATPase. Biochemistry 37: 792-799.
27. Goldstein LS (1993) With apologies to scheherazade: tails of 1001 kinesin motors. Annu Rev Genet 27: 319-351.
28. Goldstein LS (2001) Molecular motors: from one motor many tails to one motor many tales. Trends Cell Biol 11: 477-482.
29. Gurland G and Gundersen GG (1995) Stable, detyrosinated microtubules function to localize vimentin intermediate filaments in fibroblasts. J Cell Biol 131: 1275-1290.
30. Gyoeva FK and Gelfand VI (1991) Coalignment of vimentin intermediate filaments with microtubules depends on kinesin. Nature 353: 445-448.
31. Hackney DD (1994) Evidence for alternating head catalysis by kinesin during microtubule-stimulated ATP hydrolysis. Proc Natl Acad Sci USA 91: 6865-6869.
32. Hirose K, Lockhart A, Cross RA and Amos LA (1995) Nucleotide-dependent angular change in kinesin motor domain bound to tubulin. Nature 376: 277-279.
33. Hirose K, Lowe J, Alonso M, Cross RA and Amos LA (1999) 3D electron microscopy of the interaction of kinesin with tubulin. Cell Struct Funct. 24: 277-284.
34. Hoenger A and Milligan RA (1997) Motor domains of kinesin and ncd interact with microtubule protofilaments with the same binding geometry. J Mol Biol 265: 553-564.
35. Hoenger A, Sablin EP, Vale RD, Fletterick RJ and Milligan RA (1995) Three-dimensional structure of a tubulin-motor-protein complex. Nature 376: 271-274.
36. Howard J, Hudspeth AJ and Vale RD (1989) Movement of microtubules by single kinesin molecules. Nature 342: 154-158.
37. Huitorel P, White D, Fouquet JP, Kann ML, Cosson J and Gagnon C (2002) Differential distribution of glutamylated tubulin isoforms along the sea urchin sperm axoneme. Mol Reprod Dev 62: 139-148.
38. Hutchens JA, Hoyle HD, Turner FR and Raff EC (1997) Structurally similar Drosophila alpha-tubulins are functionally distinct in vivo. Mol Biol Cell 8: 481-500.
39. Idriss HT (2000) Phosphorylation of tubulin tyrosine ligase: a potential mechanism for regulation of alpha-tubulin tyrosination. Cell Motil Cytoskeleton 46: 1-5.
40. Janke C, Rogowski K, Wloga D, Regnard C, Kajava AV, Strub JM, Temurak N, van Dijk J, Boucher D, van Dorsselaer A and others (2005) Tubulin polyglutamylase enzymes are members of the TTL domain protein family. Science 308: 1758-1762.
41. Jimenez MA, Evangelio JA, Aranda C, Lopez-Brauet A, Andreu D, Rico M, Lagos R, Andreu JM and Monasterio O (1999) Helicity of alpha(404-451) and beta(394-445) tubulin C-terminal recombinant peptides. Protein Sci 8: 788-799.
42. Johnson KA (1998) The axonemal microtubules of the Chlamydomonas flagellum differ in tubulin isoform content. J Cell Sci 111: 313-320.
43. Johnson CS, Buster D and Scholey JM (1990) Light chains of sea urchin kinesin identified by immunoadsorption. Cell Motil Cytoskeleton 16: 204-213.
44. Kalisz HM, Erck C, Plessmann U and Wehland J (2000) Incorporation of nitrotyrosine into alpha-tubulin by recombinant mammalian tubulin-tyrosine ligase. Biochim Biophys Acta 1481: 131-138.
45. Kami ML, Soues S, Levilliers N and Fouquet JP (2003) Glutamylated tubulin: diversity of expression and distribution of isoforms. Cell Motil Cytoskeleton 55: 14-25.
46. Kapoor TM and Compton DA (2002) Searching for the middle ground: mechanisms of chromosome alignment during mitosis. J Cell Biol 157: 551-556.
47. Karabay A and Walker RA (1999a) Identification of microtubule binding sites in the Ncd tail domain. Biochemistry 38: 1838-1849.
48. Karabay A and Walker RA (1999b) The Ncd tail domain promotes microtubule assembly and stability. Biochem Biophys Res Commun 258: 39-43.
49. Karabay A and Walker RA (2003) Identification of Ncd tail domain-binding sites on the tubulin dimer. Biochem Biophys Res Commun 305: 523-528.
50. Kashina AS, Baskin RJ, Cole DG, Wedaman KP, Saxton WM and Scholey JM (1996) A bipolar kinesin. Nature 379: 270-272.
51. Kirchner J, Seiler S, Fuchs S and Schliwa M (1999) Functional anatomy of the kinesin molecule in vivo. EMBO J 18: 4404-4413.
52. Klopfenstein DR, Tomishige M, Stuurman N and Vale RD (2002) Role of phosphatidylinositol(4,5)bisphosphate organization in membrane transport by the Unc104 kinesin motor. Cell 109: 347-358.
53. Krebs A, Goldie KN and Hoenger A (2004) Complex formation with kinesin motor domains affects the structure of microtubules. J Mol Biol 335: 139-153.
54. Kreitzer G, Liao G and Gundersen GG (1999) Detyrosination of tubulin regulates the interaction of intermediate filaments with microtubules in vivo via a kinesin-dependent mechanism. Mol Biol Cell 10: 1105-1118.
55. Kull FJ, Sablin EP, Lau R, Fletterick RJ and Vale RD (1996) Crystal structure of the kinesin motor domain reveals a structural similarity to myosin. Nature 380: 550-555.
56. Kuznetsov SA, Vaisberg YA, Rothwell SW, Murphy DB and Gelfand VI (1989) Isolation of a 45-kDa fragment from the kinesin heavy chain with enhanced ATPase and microtubule-binding activities. J Biol Chem 264: 589-595.
57. Kuznetsov SA, Vaisberg EA, Shanina NA, Magretova NN, Chernyak VY and Gelfand VI (1988) The quaternary structure of bovine brain kinesin. EMBO J 7: 353-356.
58. Lafanechere L, Courtay-Cahen C, Kawakami T, Jacrot M, Rudiger M, Wehland J, Job D and Margolis RL (1998) Suppression of tubulin tyrosine ligase during tumor growth. J Cell Sci 111: 171-181.
59. Lakamper S and Meyhofer E (2005) The E-hook of tubulin interacts with kinesin's head to increase processivity and speed. Biophys J 89: 3223-3234.
60. Lakamper S, Kallipolitou A, Woehlke G, Schliwa M and Meyhofer E (2003) Single fungal kinesin motor molecules move processively along microtubules. Biophys J 84: 1833-1843.
61. Larcher JC, Boucher D, Lazereg S, Gros F and Denoulet P (1996) Interaction of kinesin motor domains with alpha- and beta-tubulin subunits at a tau-independent binding site. Regulation by polyglutamylation. J Biol Chem 271: 22117-22124.
62. Lawrence CJ, Dawe RK, Christie KR, Cleveland DW, Dawson SC, Endow SA, Goldstein LS, Goodson HV, Hirokawa N, Howard J and others (2004) A standardized kinesin nomenclature. J Cell Biol 167: 19-22.
63. Levilliers N, Fleury A and Hill AM (1995) Monoclonal and polyclonal antibodies detect a new type of post-translational modification of axonemal tubulin. J Cell Sci 108: 3013-3028.
64. Liao G and Gundersen GG (1998) Kinesin is a candidate for cross-bridging microtubules and intermediate filaments. Selective binding of kinesin to detyrosinated tubulin and vimentin. J Biol Chem 273: 9797-9803.
65. Luduena RF (1998) Multiple forms of tubulin: different gene products and covalent modifications. Int Rev Cytol 178: 207-275.
66. Mary J, Redeker V, Le Caer JP, Rossier J and Schmitter JM (1996) Posttranslational modifications in the C-terminal tail of axonemal tubulin from sea urchin sperm. J Biol Chem 271: 9928-9933.
67. Mencarelli C, Bre MH, Levilliers N and Dallai R (2000) Accessory tubules and axonemal microtubules of Apis mellifera sperm flagellum differ in their tubulin isoform content. Cell Motil Cytoskeleton 47: 1-12.
68. Meyhofer E and Howard J (1995) The force generated by a single kinesin molecule against an elastic load. Proc Natl Acad Sci USA 92: 574-578.
69. Mialhe A, Lafanechere L, Treilleux I, Peloux N, Dumontet C, Bremond A, Panh MH, Payan R, Wehland J, Margolis RL and others (2001) Tubulin detyrosination is a frequent occurrence in breast cancers of poor prognosis. Cancer Res 61: 5024-5027.
70. Million K, Larcher J, Laoukili J, Bourguignon D, Marano F and Tournier F (1999) Polyglutamylation and polyglycylation of alpha-and beta-tubulins during in vitro ciliated cell differentiation of human respiratory epithelial cells. J Cell Sci 112: 4357-4366.
71. Multigner L, Pignot-Paintrand I, Saoudi Y, Job D, Plessmann U, Rudiger M and Weber K (1996) The A and B tubules of the outer doublets of sea urchin sperm axonemes are composed of different tubulin variants. Biochemistry 35: 10862-10871.
72. Murofushi H (1980) Purification and characterization of tubulin-tyrosine ligase from porcine brain. J Biochem (Tokyo) 87: 979-984.
73. Muto E, Sakai H and Kaseda K (2005) Long-range cooperative binding of kinesin to a microtubule in the presence of ATP. J Cell Biol 168: 691-696.
74. Nielsen MG, Turner FR, Hutchens JA and Raff EC (2001) Axoneme-specific beta-tubulin specialization: a conserved C-terminal motif specifies the central pair. Curr Biol 11: 529-533.
75. Nitta R, Kikkawa M, Okada Y and Hirokawa N (2004) KIF1A alternately uses two loops to bind microtubules. Science 305: 678-683.
76. Nogales E, Wolf SG and Downing KH (1998) Structure of the alpha beta tubulin dimer by electron crystallography. Nature 391: 199-203.
77. Okada Y and Hirokawa N (1999) A processive single-headed motor: kinesin superfamily protein KIF1A. Science 283: 1152-1157.
78. Okada Y and Hirokawa N (2000) Mechanism of the single-headed processivity: diffusional anchoring between the K-loop of kinesin and the C terminus of tubulin. Proc Natl Acad Sci USA 97: 640-645.
79. Ovechkina Y, Wagenbach M and Wordeman L (2002) K-loop insertion restores microtubule depolymerizing activity of a "neckless" MCAK mutant. J Cell Biol 159: 557-562.
80. Paturle-Lafanechere L, Edde B, Denoulet P, Van Dorsselaer A, Mazarguil H, Le Caer JP, Wehland J and Job D (1991) Characterization of a major brain tubulin variant which cannot be tyrosinated. Biochemistry 30: 10523-10528.
81. Plessmann U and Weber K (1997) Mammalian sperm tubulin: an exceptionally large number of variants based on several posttranslational modifications. J Protein Chem 16: 385-390.
82. Purro SA, Bisig CG, Contin MA, Barra HS and Arce CA (2003) Posttranslational incorporation of the antiproliferative agent azatyrosine into the C-terminus of alpha-tubulin. Biochem J 375: 121-129.
83. Raff EC, Fackenthal JD, Hutchens JA, Hoyle HD and Turner FR (1997) Microtubule architecture specified by a beta-tubulin isoform. Science 275: 70-73.
84. Raybin D and Flavin M (1977a) Enzyme which specifically adds tyrosine to the alpha chain of tubulin. Biochemistry 16: 2189-2194.
85. Raybin D and Flavin M (1977b) Modification of tubulin by tyrosylation in cells and extracts and its effect on assembly in vitro. J Cell Biol 73: 492-504.
86. Redeker V, Frankfurter A, Parker SK, Rossier J, Detrich HW and 3rd (2004) Posttranslational modification of brain tubulins from the Antarctic fish Notothenia coriiceps: reduced C-terminal glutamylation correlates with efficient microtubule assembly at low temperature. Biochemistry 43: 12265-12274.
87. Redeker V, Levilliers N, Schmitter JM, Le Caer JP, Rossier J, Adoutte A and Bre MH (1994) Polyglycylation of tubulin: a posttranslational modification in axonemal microtubules. Science 266: 1688-1691.
88. Redeker V, Levilliers N, Vinolo E, Rossier J, Jaillard D, Burnette D, Gaertig J and Bre MH (2005) Mutations of tubulin glycylation sites reveal cross-talk between the C termini of alpha- and beta-tubulin and affect the ciliary matrix in Tetrahymena. J Biol Chem 280: 596-606.
89. Rice S, Lin AW, Safer D, Hart CL, Naber N, Carragher BO, Cain SM, Pechatnikova E, Wilson-Kubalek EM, Whittaker M and others (1999) A structural change in the kinesin motor protein that drives motility. Nature 402: 778-784.
90. Rudiger M, Plessmann U, Rudiger AH and Weber K (1995) Beta tubulin of bull sperm is polyglycylated. FEBS Lett 364: 147-151.
91. Sackett DL, Bhattacharyya B and Wolff J (1985) Tubulin subunit carboxyl termini determine polymerization efficiency. J Biol Chem 260: 43-45.
92. Schafer F, Deluca D, Majdic U, Kirchner J, Schliwa M, Moroder L and Woehlke G (2003) A conserved tyrosine in the neck of a fungal kinesin regulates the catalytic motor core. EMBO J 22: 450-458.
93. Scholey JM, Heuser J, Yang JT and Goldstein LS (1989) Identification of globular mechanochemical heads of kinesin. Nature 338: 355-357.
94. Seiler S, Kirchner J, Horn C, Kallipolitou A, Woehlke G and Schliwa M (2000) Cargo binding and regulatory sites in the tail of fungal conventional kinesin. Nat Cell Biol 2: 333-338.
95. Seitz A, Kojima H, Oiwa K, Mandelkow EM, Song YH and Mandelkow E (2002) Single-molecule investigation of the interference between kinesin, tau and MAP2c. EMBO J 21: 4896-4905.
96. Shiroguchi K, Ohsugi M, Edamatsu M, Yamamoto T and Toyoshima YY (2003) The second microtubule-binding site of monomeric kid enhances the microtubule affinity. J Biol Chem 278: 22460-22465.
97. Skiniotis G, Cochran JC, Muller J, Mandelkow E, Gilbert SP and Hoenger A (2004) Modulation of kinesin binding by the C-termini of tubulin. EMBO J 23: 989-999.
98. Song YH and Mandelkow E (1993) Recombinant kinesin motor domain binds to beta-tubulin and decorates microtubules with a B surface lattice. Proc Natl Acad Sci USA 90: 1671-1675.
99. Song YH, Marx A, Muller J, Woehlke G, Schliwa M, Krebs A, Hoenger A and Mandelkow E (2001) Structure of a fast kinesin: implications for ATPase mechanism and interactions with microtubules. EMBO J 20: 6213-6225.
100. Stock MF, Chu J and Hackney DD (2003) The kinesin family member BimC contains a second microtubule binding region attached to the N terminus of the motor domain. J Biol Chem 278: 52315-52322.
101. Sullivan KF and Cleveland DW (1986) Identification of conserved isotype-defining variable region sequences for four vertebrate beta tubulin polypeptide classes. Proc Natl Acad Sci USA 83: 4327-4331.
102. Svoboda K, Schmidt CF, Schnapp BJ and Block SM (1993) Direct observation of kinesin stepping by optical trapping interferometry. Nature 365: 721-727.
103. Thazhath R, Liu C and Gaertig J (2002) Polyglycylation domain of beta-tubulin maintains axonemal architecture and affects cytokinesis in Tetrahymena. Nat Cell Biol 4: 256-259.
104. Thorn KS, Ubersax JA and Vale RD (2000) Engineering the processive run length of the kinesin motor. J Cell Biol 151: 1093-1100.
105. Tucker C and Goldstein LS (1997) Probing the kinesin-microtubule interaction. J Biol Chem 272: 9481-9488.
106. Vale RD (2003) The molecular motor toolbox for intracellular transport. Cell 112: 467-480.
107. Vale RD, Coppin CM, Malik F, Kull FJ and Milligan RA (1994) Tubulin GTP hydrolysis influences the structure, mechanical properties, and kinesin-driven transport of microtubules. J Biol Chem 269: 23769-23775.
108. Vale RD, Funatsu T, Pierce DW, Romberg L, Harada Y and Yanagida T (1996) Direct observation of single kinesin molecules moving along microtubules. Nature 380: 451-453.
109. Vale RD, Reese TS and Sheetz MP (1985) Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility. Cell 42: 39-50.
110. Verhey KJ, Lizotte DL, Abramson T, Barenboim L, Schnapp BJ and Rapoport TA (1998) Light chain-dependent regulation of Kinesin's interaction with microtubules. J Cell Biol 143: 1053-1066.
111. Verhey KJ and Rapoport TA (2001) Kinesin carries the signal. Trends Biochem Sci 26: 545-550.
112. Wang Z and Sheetz MP (2000) The C-terminus of tubulin increases cytoplasmic dynein and kinesin processivity. Biophys J 78: 1955-1964.
113. Webster DR, Wehland J, Weber K and Borisy GG (1990) Detyrosination of alpha tubulin does not stabilize microtubules in vivo. J Cell Biol 111: 113-122.
114. Wendt T, Karabay A, Krebs A, Gross H, Walker R and Hoenger A (2003) A structural analysis of the interaction between ncd tail and tubulin protofilaments. J Mol Biol 333: 541-552.
115. Westermann S and Weber K (2003) Post-translational modifications regulate microtubule function. Nat Rev Mol Cell Biol 4: 938-947.
116. Woehlke G (2001) A look into kinesin's powerhouse. FEBS Lett 508: 291-294.
117. Woehlke G, Ruby AK, Hart CL, Ly B, Hom-Booher N and Vale RD (1997) Microtubule interaction site of the kinesin motor. Cell 90: 207-216.
118. Xia L, Hai B, Gao Y, Burnette D, Thazhath R, Duan J, Bre MH, Levilliers N, Gorovsky MA and Gaertig J (2000) Polyglycylation of tubulin is essential and affects cell motility and division in Tetrahymena thermophila. J Cell Biol 149: 1097-1106.
119. Yildiz A, Tomishige M, Vale RD and Selvin PR (2004) Kinesin walks hand-over-hand. Science 303: 676-678.
120. Yoshiyama Y, Zhang B, Bruce J, Trojanowski JQ and Lee VM (2003) Reduction of detyrosinated microtubules and Golgi fragmentation are linked to tau-induced degeneration in astrocytes. J Neurosci 23: 10662-10671.