3D electron microscopy of the interaction of kinesin with tubulin

Cell Structure and Function

Volumn 24, Issue 5, 1999, Pages 277-284

  Hirose, Keiko ^a   Löwe, Jan ^b   Alonso, Maria ^c   Cross, Robert A ^c   Amos, Linda A ^b  

^a NATL INST FOR ADV INTERDISC RES   (Japan)

^b MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

^c MARIE CURIE RESEARCH INSTITUTE   (United Kingdom)

Author keywords

3 D image reconstruction; Atomic structure; Cryo electron microscopy; Motility

Indexed keywords

ADENOSINE DIPHOSPHATE; ADENOSINE PHOSPHATE; ADENYLYLIMIDODIPHOSPHATE; KINESIN; TUBULIN;

ANIMAL TISSUE; CONFERENCE PAPER; CONTROLLED STUDY; ELECTRON MICROSCOPY; MICROTUBULE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; THREE DIMENSIONAL IMAGING;

ADENOSINE DIPHOSPHATE; ANIMALS; APYRASE; BINDING SITES; CRYOELECTRON MICROSCOPY; FOURIER ANALYSIS; HEXOKINASE; IMAGE PROCESSING, COMPUTER-ASSISTED; IMAGING, THREE-DIMENSIONAL; KINESIN; MICROSCOPY, ELECTRON; MICROSCOPY, ELECTRON, SCANNING; MICROTUBULE PROTEINS; MICROTUBULES; MODELS, MOLECULAR; MOLECULAR MOTOR PROTEINS; PACLITAXEL; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; SWINE; TUBULIN;

EID: 0345073110     PISSN: 03867196     EISSN: None     Source Type: Journal    
DOI: 10.1247/csf.24.277     Document Type: Conference Paper

References (36)

1. ALONSO, M.C., VANDERKERCKHOVE, J., and CROSS, R.A. 1998. Proteolytic mapping of kinesin/ncd-microtubule interface: nucleotide-dependent conformational changes in the loops L8 and L12. EMBO J., 17: 945-951.
2. AMOS, L.A. and HIROSE, K. 1997. The structure of microtubule-motor complexes. Curr. Opin. Cell Biol., 9: 4-11.
3. CASE, R.B., PIERCE, D.W., HOM-BOOHER, N., HART, C.L., and VALE, R.D. 1998. The directional preference of kinesin motors is specified by an element outside of the motor catalytic domain. Cell, 90: 959-966.
4. DESAI, A., VERMA, S., MITCHISON, T.J., and WALCZAK, C.E. 1999. Kin 1 kinesins are microtubule-destabilising enzymes. Cell, 96: 69-78.
5. ENDOW, S.A., HENIKOFF, S., and SOLER NIEDZIELA, L. 1990. Mediation of meiotic and early mitotic chromosome segregation in Drosophila by a protein related to kinesin. Nature, 345: 81-83.
6. ENDOW, S.A. and WALIGORA, K.W. 1998. Determinants of kinesin motor polarity. Science, 281: 1200-1202.
7. FAN, J., GRIFFITHS, A.D., LOCKHART, A., CROSS, R.A., and AMOS, L.A. 1996b. Microtubule minus ends can be labelled with a phage display antibody specific to alpha-tubulin. J. Mol. Biol., 259: 325-330.
8. GOLDSTEIN, L.S.B. 1993. With apologies to Scheherazade -tails of 1001 kinesin motors. Ann. Rev. Genetics, 27: 319-351.
9. HENNINGSEN, U. and SCHLIWA, M. 1997. Reversal of the direction of movement of a molecular motor. Nature, 389: 93-96.
10. HIROKAWA, N. 1998. Kinesin and dynein superfamily proteins and the mechanism of organelle transport. Science, 279: 519-526.
11. HIROSE, K., LOCKHART, A., CROSS, R.A., and AMOS, L.A. 1995. Nucleotide-dependent angular change in kinesin motor domain bound to tubulin. Nature (London), 376: 277-279.
12. HIROSE, K., LOCKHART, A., CROSS, R.A., and AMOS, L.A. 1996. Three-dimensional cryoelectron microscopy of dimeric kinesin and ncd motor domains on microtubules. Proc. Natl. Acad. Sci. USA, 93: 9539-9544.
13. HIROSE, K., AMOS, W.B., LOCKHART, A., CROSS, R.A., and AMOS, L.A. 1997. Three-dimensional cryoelectron microscopy of 16-protofilament microtubules: structure, polarity and interaction with motor protein. J. Struct. Biol., 118: 140-148.
14. HIROSE, K., CROSS, R.A., and AMOS, L.A. 1998. Nucleotide-dependent structural changes in dimeric ncd molecules complexed to microtubules. J. Mol. Biol., 278: 389-400.
15. HIROSE, K., LÖWE, J., ALONSO, M., CROSS, R.A., and AMOS, L.A. 1999. Congruent docking of dimeric kinesin and ncd into 3-D electron cryo-microscopy maps of microtubule-motor. ADP complexes. Mol. Biol. Cell, 10: 2063-2074.
16. HOENGER, A. and MILLIGAN, R.A. 1997. Motor domains of kinesin and ncd interact with microtubule protofilaments with the same binding geometry. J. Mol. Biol., 265: 553-564.
17. HOENOER, A., SACK, S., THORMÄHLEN, M., MARX, A., MÜLLER, J., GROSS, H., and MANDELKOW, E. 1998. Image reconstructions of microtubules decorated with monomeric and dimeric kinesins: comparison with x-ray structure and implications for motility. J. Cell Biol., 141: 419-430.
18. KOZIELSKI, F., SACK, S., MARX, A., THORMÄHLEN, M., SCHÖNBRUNN, E., BIOU, V., THOMPSON, A., MANDELKOW, E.M., and MANDELKOW, E. 1997. The crystal structure of dimeric kinesin and implications for microtubule-dependent motility. Cell, 91: 985-994.
19. KOZIELSKI, F. ARNAL, I., and WADE, R.H. 1998. A model of the microtubule-kinesin complex based on electron cryomicroscopy and X-ray crystallography. Current Biology, 8: 191-198.
20. KRAULIS, P.J. 1991. Molscript - a program to produce both detailed and schematic plots of protein structures. J. Appl. Crystaltogr., 24: 946-950.
21. KULL, F.J., SABLIN, E.P., LAU, R., FLETTERICK, R.J., and VALE, R.D. 1996. Crystal structure of the kinesin motor domain reveals a structural similarity to myosin. Nature (London), 380: 550-555.
22. LOCKHART, A., CREVEL, I.M.-T.C., and CROSS, R.A. 1995. Kinesin and ncd bind through a single head to microtubules and compete for a shared MT binding site. J. Mol. Biol., 249: 763-771.
23. MITCHISON, T.J. 1993. Localization of an exchangeable GTP-binding site at the plus end of microtubules. Science, 261: 1044-1047.
24. NOGALES, E., DOWNING, K.H., AMOS, L.A., and LÖWE, J. 1998. Tubulin and FtsZ form a distinct family of GTPases. Nature Structural Biology, 5: 451-458.
25. NOGALES, E., WOLF, S., and DOWNING, K.H. 1998. Structure of the αβ-tubulin dimer by electron crystallography. Nature, 391: 199-203.
26. NOGALES, E., WHITTAKER, M., MILLIGAN, R.A., and DOWNING, K.H. 1999. High resolution model of the microtubule. Cell, 96: 79-88.
27. RAY, S., MEYHÖFER, E., MILLIGAN, R.A., and HOWARD, J. 1993. Kinesin follows the microtubule's protofilament aixs. J. Cell Biol., 121: 1083-1093.
28. SABLIN, E.P., KULL, F.J., COOKE, R., VALE, R.D., and FLETTERICK, R.J. 1996. Crystal structure of the motor domain of the kinesin-related motor ncd. Nature (London), 380: 555-559.
29. SABLIN, E.P., CASE, R.B., DAI, S.C., HART, C.L., RUBY, A., FLETTERICK, R.J., and VALE, R.D. 1998. Direction determination in the minus-end-directed kinesin motor ncd. Nature, 395: 813-816.
30. SACK, S., MÜLLER, J., MARX, A., THORMÄHLEN, M., MANDELKOW, E.M., BRADY, S.T., and MANDELKOW, E. 1997a. X-ray structure of motor and neck domains from rat brain kinesin. Biochemistry, 36: 16155-16165.
31. SONG, Y.-H. and MANDELKOW, E. 1995. The anatomy of flagellar microtubules: polarity, seam, junctions, and lattice. J. Cell Biol., 128: 81-94.
32. SOSA, H., DIAS, D.P., HOENGER, A., WHITTAKER, M., WILSON-KUBALEK, E., SABLIN, E., FLETTERICK, R.J., VALE, R.D., and MILLIAGN, R.A. 1997. A model for the microtubule-ncd motor protein complex obtained by cryo-electron microscopy and image analysis. Cell, 90: 217-224.
33. TURK, D. 1992. Weiterentwicklung eines programms für molekülgraphik und elektrondichte-manipulation und seine anwendung auf verschiedene protein-strukturaufklärungen: Technische Universität, München.
34. VALE, R.D., REESE, T.S., and SHEETZ, M.P. 1985. Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility. Cell, 42: 39-50.
35. VALE, R.D. and FLETTERICK, R.J. 1997. The design plan of kinesin motors. Annu. Rev. Cell Dev. Biol., 13: 745-777.
36. WOEHLKE, G., RUBY, A.K., HART, C.L., LY, B., HOMBOOHER, N., and VALE, R.D. 1997. Microtubule interaction site of the kinesin motor. Cell, 90: 207-216.