Tolbutamide reduces glioma cell proliferation by increasing connexin43, which promotes the up-regulation of p21 and p27 and subsequent changes in retinoblastoma phosphorylation

GLIA

Volumn 54, Issue 2, 2006, Pages 125-134

  Sánchez Alvarez, Rosa ^a   Paíno, Teresa ^a   Herrero González, Sandra ^a   Medina, José M ^a   Tabernero, Arantxa ^a  

^a UNIVERSITY OF SALAMANCA   (Spain)

Author keywords

Astrocytes; cAMP; Cell cycle; Gap junctions; Glia; Proliferation

Indexed keywords

CONNEXIN 43; CYCLIN D1; CYCLIN D3; KI 67 ANTIGEN; PROTEIN P21; PROTEIN P27; SMALL INTERFERING RNA; TOLBUTAMIDE; TUMOR SUPPRESSOR PROTEIN;

ANIMAL CELL; ARTICLE; CELL PROLIFERATION; GLIOMA CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; RETINOBLASTOMA;

ANIMALS; CELL PROLIFERATION; CONNEXIN 43; CYCLIN-DEPENDENT KINASE INHIBITOR P21; CYCLIN-DEPENDENT KINASE INHIBITOR P27; GLIOMA; PHOSPHORYLATION; RATS; RETINOBLASTOMA; TOLBUTAMIDE; TUMOR CELLS, CULTURED; UP-REGULATION;

EID: 33746041455     PISSN: 08941491     EISSN: 10981136     Source Type: Journal    
DOI: 10.1002/glia.20363     Document Type: Article

References (43)

1. Azarnia R, Dahl G, Loewenstein WR. 1981. Cell junction and cycle AMP. III. Promotion of junctional membrane permeability and junctional membrane particles in a junction-deficient cell type. J Membr Biol 63:133-146.
2. Bertram JS. 2000. The molecular biology of cancer. Mol Aspects Med 21:167-223.
3. Beyer EC, Paul DL, Goodenough DA. 1987. Connexin43: A protein from rat heart homologous to a gap junction protein from liver. J Cell Biol 105:2621-2629.
4. Boylan JF, Sharp DM, Leffet L, Bowers A, Pan W. 1999. Analysis of site-specific phosphorylation of the retinoblastoma protein during cell cycle progression. Exp Cell Res 248:110-114.
5. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254.
6. Burghardt RC, Barhoumi R, Sewall TC, Bowen JA. 1995. Cyclic AMP induces rapid increases in gap junction permeability and changes in the cellular distribution of connexin43. J Membr Biol 148:243-253.
7. Caskey LS, Fuller GN, Bruner JM, Yung WK, Sawaya RE, Holland EC, Zhang W. 2000. Toward a molecular classification of the gliomas: Histopathology, molecular genetics, and gene expression profiling. Histol Histopathol 15:971-981.
8. Connell-Crowley L, Harper JW, Goodrich DW. 1997. Cyclin D1/Cdk4 regulates retinoblastoma protein-mediated cell cycle arrest by site-specific phosphorylation. Mol Biol Cell 8:287-301.
9. +-induced increase in gap junctional communication between mouse spinal cord astrocytes. J Neurosci 21:6635-6643.
10. Driscoll B, T'Ang A, Hu YH, Yan CL, Fu Y, Luo Y, Wu KJ, Wen S, Shi XH, Barsky L, Weinberg K, Murphree AL, Fung YK. 1999. Discovery of a regulatory motif that controls the exposure of specific upstream cyclin-dependent kinase sites that determine both conformation and growth suppressing activity of pRb. J Biol Chem 274:9463-9471.
11. Endl E, Gerdes J. 2000. The Ki-67 protein: Fascinating forms and an unknown function. Exp Cell Res 257:231-237.
12. + concentrations. J Neurochem 62:489-495.
13. Gerdes J, Li L, Schlueter C, Duchrow M, Wohlenberg C, Gerlach C, Stahmer I, Kloth S, Brandt E, Flad HD. 1991. Immunobiochemical and molecular biologic characterization of the cell proliferation-associated nuclear antigen that is defined by monoclonal antibody Ki-67. Am J Pathol 138:867-873.
14. Giaume C, Fromaget C, El Aoumari A, Cordier J, Glowinski J, Gros D. 1991. Gap junction in cultured astrocytes: Single channel currents and characterization of channel-forming protein. Neuron 6:133-143.
15. 2+ on gap junction permeability in cultured astrocytes. FEBS Lett 427:41-45.
16. Harbour JW, Dean DC. 2000. The Rb/E2F pathway: Expanding roles and emerging paradigms. Genes Dev 14:2393-2409.
17. Huang R, Fan Y, Hossain M, Peng A, Zeng Z, Boynton A. 1998. Reversion of the neoplastic phenotype of human glioblastoma cells by connexin 43 (cx43). Cancer Res 58:5089-5096.
18. Huang R-P, Hossain M, Sehgal A, Boynton A. 1999. Reduced connexin43 expression in high-grade human brain glioma cells. J Surg Oncol 70:21-24.
19. Kamei J, Toyofuku T, Hori M. 2003. Negative regulation of p21 by β-catenin/TCF signaling: A novel mechanism by which cell adhesion molecules regulate cell proliferation. Biochem Biophys Res Commun 312:380-387.
20. Loewenstein WR, Kanno Y. 1966. Intercellular communication and the control of tissue growth: Lack of communication between cancer cells. Nature 209:1248-1249.
21. Metha PP, Hotz-Wagenblatt A, Rose B, Shalloway D, Lowenstein WR. 1991. Incorporation of the gene for a cell-cell channel protein into transformed cells leads to normalization of growth. J Membr Biol 124:207-225.
22. Naus CC. 2002. Gap junctions and tumour progression. Can J Physiol Pharmacol 80:136-141.
23. Paulson AF, Lampe PD, Meyer RA, TenBroek E, Atkinson MM, Walseth TF, Johnson RG. 2000. Cyclic AMP and LDL trigger a rapid enhancement in gap junction assembly through a stimulation of connexin trafficking. J Cell Sci 113:3037-3049.
24. Pedram A, Razandi M, Hu R, Levin E. 1998. Astrocyte progression from G1 to S phase of the cell cycle depends upon multiple protein interaction. J Biol Chem 273:13966-13972.
25. Pu P, Xia Z, Yu S, Huang Q. 2004. Altered expression of Cx43 in astrocytic tumors. Clin Neurol Neurosurg 107:49-54.
26. Robe PA, Princen F, Martin D, Malgrange B, Stevenaert A, Moonen G, Gielen J, Merville M, Bours V. 2000. Pharmacological modulation of the bystander effect in the herpes simplex virus thymidine kinase/ganciclovir gene therapy system: Effects of dibutyryl adenosine 3′, 5′-cyclic monophosphate, α-glycyrrhetinic acid, and cytosine arabinoside. Biochem Pharmacol 60:241-249.
27. Sánchez-Alvarez R, Tabernero A, Medina JM. 2005. The increase in gap junctional communication decreases the rate of glucose uptake in C6 glioma cells by releasing hexokinase from mitochondria. Brain Res 1039:189-198.
28. Sánchez-Alvarez R, Tabernero A, Sanchez-Abarca LI, Orfao A, Giaume C, Medina JM. 2001. Proliferation of C6 glioma cells is blunted by the increase in gap junction communication caused by tolbutamide. FEBS Lett 509:202-206.
29. Shao Q, Wang H, McLachlan E, Veitch GI, Laird DW. 2005. Down-regulation of Cx43 by retroviral delivery of small interfering RNA promotes an aggressive breast cancer cell phenotype. Cancer Res 65:2705-2711.
30. Sherr CJ. 1995. D-type cyclins. Trends Biochem Sci 20:187-190.
31. Shinoura N, Chen L, Wani MA, Kim YG, Larson JJ, Warnick RE, Simon M, Menon AG, Bi WL, Stambrook PJ. 1996. Protein and messenger RNA expression of connexin43 in astrocytomas: Implications in brain tumor gene therapy. J Neurosurg 84:839-845.
32. Soroceanu L, Manning T, Sontheimer H. 2001. Reduced expression of connexin-43 and functional gap junction coupling in human gliomas. Glia 33:107-117.
33. Su YA, Bittner ML, Chen Y, Tao L, Jiang Y, Zhang Y, Stephan DA, Trent JM. 2000. Identification of tumor-suppressor genes using human melanoma cell lines UACC903, UACC903(+6), and SRS3 by comparison of expression profiles. Mol Carcinog 28:119-127.
34. Tabernero A, Jimenez C, Velasco A, Giaume C, Medina JM. 2001. The enhancement of glucose uptake caused by the collapse of gap junction communication is due to an increase in astrocyte proliferation. J Neurochem 78:890-898.
35. Teixeira A, Chaverot N, Strosberg A, Cazaubon S. 2000. Differential regulation of cyclin D1 and D3 expression in the control of astrocyte proliferation induced by endothelin-1. J Neurochem 74:1034-1040.
36. 2+-independent mechanism. J Neurochem 74:1249-1256.
37. Weinberg RA. 1995. The retinoblastoma protein and cell cycle control. Cell 81:323-330.
38. Yamasaki H, Naus C. 1996. Role of connexin genes in growth control. Carcinogenesis 17:1199-1213.
39. Yamasaki H, Omori Y, Krutovskikh V, Zhu W, Mironov N, Yamakage K, Mesnil M. 1999. Connexins in tumour suppression and cancer therapy. Novartis Found Symp 219:241-254.
40. Zhang X, Zhao M, Huang AY, Fei Z, Zhang W, Wang XL. 2005. The effect of cyclin D expression on cell proliferation in human gliomas. J Clin Neurosci 12:166-168.
41. Zhang YW, Morita I, Ikeda M, Ma KW, Murota S. 2001. Connexin43 suppresses proliferation of osteosarcoma U2OS cells through posttranscriptional regulation of p27. Oncogene 20:4138-4149.
42. Zhang YW, Nakayama K, Morita I. 2003. A novel route for connexin 43 to inhibit cell proliferation: Negative regulation of S-phase kinase-associated protein (Skp 2). Cancer Res 63:1623-1630.
43. Zhu D, Caveney S, Kidder GM, Naus CCG. 1991. Transfection of C6 glioma-cells with connexin-43 cDNA: Analysis of expression, intercellular coupling, and cell-proliferation. Proc Natl Acad Sci USA 88:1883-1887.