In vivo cytogenetic damage revealed by FISH analysis of micronuclei in uncultured human T lymphocytes

Cytogenetics and Cell Genetics

Volumn 75, Issue 2-3, 1996, Pages 151-154

  Surralles J ^a,b   Falck, G ^a   Norppa, H ^a  

^a FINNISH INSTITUTE OF OCCUPATIONAL HEALTH   (Finland)

^b LEIDEN UNIVERSITY   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHALASIN B;

ADULT; AGED; ARTICLE; CHROMOSOME DAMAGE; CYTOGENETICS; FEMALE; FLUORESCENCE IN SITU HYBRIDIZATION; HUMAN; HUMAN CELL; LYMPHOCYTE CULTURE; MICRONUCLEUS; NORMAL HUMAN; PRIORITY JOURNAL; T LYMPHOCYTE; X CHROMOSOME;

ADULT; AGED; CELL DIVISION; CELLS, CULTURED; CHROMOSOME ABERRATIONS; CYTOCHALASIN B; FEMALE; HUMANS; MICRONUCLEI, CHROMOSOME-DEFECTIVE; MIDDLE AGED; T-LYMPHOCYTES; X CHROMOSOME;

EID: 0030462549     PISSN: 03010171     EISSN: 1424859X     Source Type: Journal    
DOI: 10.1159/000134467     Document Type: Article

References (13)

1. Becker P, Scherthan H, Zankl H: Use of centromere-specific DNA probe (p82H) in nonisotopic in situ hybridization for classification of micronuclei, Genes Chrom Cancer 2:59-62 (1990).
2. Catalán J, Autio K, Wessman M, Lindholm C, Knuutila S, Sorsa M, Norppa H: Age-associated micronuclei containing centromeres and the X chromosome in lymphocytes of women, Cytogenet Cell Genet 68:11-16 (1995).
3. Falck G, Catalán J, Norppa H: Influence of culture time on the frequency and contents of human lymphocyte micronuclei with and without cytochalasin B, Mutat Res (1996, in press).
4. Fenech M, Morley AA: Measurement of micronuclei in lymphocytes, Mutat Res 147:29-36 (1985).
5. Guttenbach M, Schakowski R, Schmid M: Ancuploidy and ageing: sex chromosome exclusion into micronuclei, Hum Genet 94:295-298 (1994).
6. Hando JC, Nath J, Tucker JD: Sex chromosomes, micronuclei and aging in women, Chromosoma 103:186-192(1994).
7. Meyne J, Littlefield LG, Moyzis RK: labeling of human centromeres using an alphoid consensus sequence: application to the scoring of chromosome aberrations, Mutat Res 226:75-79 (1989).
8. Miltenyi S, Muller W, Weichel W, Radbruch A: High gradient magnetic cell separation with MACS, Cytometry 11:231-238(1990).
9. Norppa H, Renzi L, Lindholm C: Detection of whole chromosomes in micronuclci of eytokinesis-blocked human lymphocytes by antikinetochore staining and in situ hybridization, Mutagenesis 6:519-525(1993).
10. Pinkel D, Straume T, Gray JW: Cytogenetic analysis using quantitative, high sensitivity, fluorescence hybridization, Proc natl Acad Sci, USA 83:2934-2938(1986).
11. Richard F, Muleris M, Dutrillaux B: The frequency of micronuclei with X chromosomes increases with age in human females, Mutat Res 316:1-7(1994).
12. Surrallés J, Catalan J, Crcus A, Norppa H, Xamena N, Mareos R: Micronuelei induced by alachlor, mito-myein-C and vinblastine in human lymphocytes: presence of centromeres and kinetochores and influence of staining technique, Mutagenesis 10:417-423(1995).
13. Tucker JD, Nath J, Hando JC: Activation status of the X chromosome in human micronucleated lymphocytes, Hunt Genet 97:471-475 (1996).