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Neural and immunological synaptic relations
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A novel adapter protein orchestrates receptor patterning and cytoskeletal polarity in T cell contacts
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Dustin M.L., Olszowy M.W., Holdorf A.D., Li J., Bromley S., Desai N., Widder P., Rosenberger F., van der Merwe P.A., Allen P.M., et al. A novel adapter protein orchestrates receptor patterning and cytoskeletal polarity in T cell contacts. Cell 94 (1998) 667-677
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Costimulation: building an immunological synapse
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Dustin M.L., and Shaw A.S. Costimulation: building an immunological synapse. Science 283 (1999) 649-650
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Dustin, M.L.1
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The immunological synapse: a molecular machine controlling T cell activation
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Grakoui A., Bromley S.K., Sumen C., Davis M.M., Shaw A.S., Allen P.M., and Dustin M.L. The immunological synapse: a molecular machine controlling T cell activation. Science 285 (1999) 221-227
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Grakoui, A.1
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Differential clustering of CD4 and CD3ζ during T cell recognition
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Krummel M.F., Sjaastad M.D., Wulfing C., and Davis M.M. Differential clustering of CD4 and CD3ζ during T cell recognition. Science 289 (2000) 1349-1352
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T cell receptor signaling precedes immunological synapse formation
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Lee K.H., Holdorf A.D., Dustin M.L., Chan A.C., Allen P.M., and Shaw A.S. T cell receptor signaling precedes immunological synapse formation. Science 295 (2002) 1539-1542
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Lee, K.H.1
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12
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The immunological synapse balances T cell receptor signaling and degradation
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Lee K.H., Dinner A.R., Tu C., Campi G., Raychaudhuri S., Varma R., Sims T.N., Burack W.R., Wu H., Wang J., et al. The immunological synapse balances T cell receptor signaling and degradation. Science 302 (2003) 1218-1222
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Lee, K.H.1
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T cell receptor (TCR) clustering in the immunological synapse integrates TCR and costimulatory signaling in selected T cells
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Purtic B., Pitcher L.A., van Oers N.S., and Wulfing C. T cell receptor (TCR) clustering in the immunological synapse integrates TCR and costimulatory signaling in selected T cells. Proc Natl Acad Sci USA 102 (2005) 2904-2909
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0036794399
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Staging and resetting T cell activation in SMACs
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Freiberg B.A., Kupfer H., Maslanik W., Delli J., Kappler J., Zaller D.M., and Kupfer A. Staging and resetting T cell activation in SMACs. Nat Immunol 3 (2002) 911-917
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27544441784
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Actin and agonist MHC-peptide complex-dependent T cell receptor microclusters as scaffolds for signaling
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This was the first study to use total internal reflection fluorescence microscopy to detect TCR clusters in the periphery of the IS after cSMAC formation that are highly active in early TCR signaling. TCR cluster formation is actin-dependent but highly resistant to inhibition by PP2, an inhibitor of src family kinases.
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Campi G., Varma R., and Dustin M.L. Actin and agonist MHC-peptide complex-dependent T cell receptor microclusters as scaffolds for signaling. J Exp Med 202 (2005) 1031-1036. This was the first study to use total internal reflection fluorescence microscopy to detect TCR clusters in the periphery of the IS after cSMAC formation that are highly active in early TCR signaling. TCR cluster formation is actin-dependent but highly resistant to inhibition by PP2, an inhibitor of src family kinases.
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J Exp Med
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Campi, G.1
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30044441433
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Newly generated T cell receptor microclusters initiate and sustain T cell activation by recruitment of Zap70 and SLP-76
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The authors use total internal reflection fluorescence microscopy to demonstrate the continuous formation and translocation of TCR microclusters. These recruit ZAP-70 and SLP-76 in the periphery and appear to release them or become internalized in the center.
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Yokosuka T., Sakata-Sogawa K., Kobayashi W., Hiroshima M., Hashimoto-Tane A., Tokunaga M., Dustin M.L., and Saito T. Newly generated T cell receptor microclusters initiate and sustain T cell activation by recruitment of Zap70 and SLP-76. Nat Immunol 6 (2005) 1253-1262. The authors use total internal reflection fluorescence microscopy to demonstrate the continuous formation and translocation of TCR microclusters. These recruit ZAP-70 and SLP-76 in the periphery and appear to release them or become internalized in the center.
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Nat Immunol
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Varma R, Campi G, Yokosuka T, Saito T, Dustin ML: Compartmentalization of signaling and degradation in the immunological synapse. Immunity 2006. in press. This study demonstrates that disruption of TCR microcluster formation blocked signaling within the lifetime of the microclusters (two minutes). The cSMAC is found to be highly enriched in lysobisphosphatidic acid - a lipid associated with sorting of ubiquitinated proteins. CD45 is excluded from TCR microclusters, but is included in the cSMAC.
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18
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9144273746
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Role of LBPA and Alix in multivesicular liposome formation and endosome organization
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Matsuo H., Chevallier J., Mayran N., Le Blanc I., Ferguson C., Faure J., Blanc N.S., Matile S., Dubochet J., Sadoul R., et al. Role of LBPA and Alix in multivesicular liposome formation and endosome organization. Science 303 (2004) 531-534
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The dendritic cell cytoskeleton is critical for the formation of the immunological synapse
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Al-Alwan M.M., Rowden G., Lee T.D., and West K.A. The dendritic cell cytoskeleton is critical for the formation of the immunological synapse. J Immunol 166 (2001) 1452-1456
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Dendritic cell maturation controls adhesion, synapse formation, and the duration of the interactions with naïve T lymphocytes
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Benvenuti F., Lagaudriere-Gesbert C., Grandjean I., Jancic C., Hivroz C., Trautmann A., Lantz O., and Amigorena S. Dendritic cell maturation controls adhesion, synapse formation, and the duration of the interactions with naïve T lymphocytes. J Immunol 172 (2004) 292-301
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21
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20844439947
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Multifocal structure of the T cell-dendritic cell synapse
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This electron microscopy study in T cell-DC IS reveals that mature DCs can form multifocal IS, the generation of which is highly correlated with full T cell activation.
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Brossard C., Feuillet V., Schmitt A., Randriamampita C., Romao M., Raposo G., and Trautmann A. Multifocal structure of the T cell-dendritic cell synapse. Eur J Immunol 35 (2005) 1741-1753. This electron microscopy study in T cell-DC IS reveals that mature DCs can form multifocal IS, the generation of which is highly correlated with full T cell activation.
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Subcellular localization of CD80 receptors is dependent on an intact cytoplasmic tail and is required for CD28-dependent T cell costimulation
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CD80 cytoplasmic domain controls localization of CD28, CTLA-4, and protein kinase C-θ in the immunological synapse
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In this study, the authors demonstrate that CD28-CD80 interactions segregate from TCR-MHCp interactions in the IS formed between T cells and CHO cells. Segregation is dependent upon the cytoplasmic domain of CD80 and correlates with enhanced costimulatory activity.
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Tseng S.Y., Liu M., and Dustin M.L. CD80 cytoplasmic domain controls localization of CD28, CTLA-4, and protein kinase C-θ in the immunological synapse. J Immunol 175 (2005) 7829-7836. In this study, the authors demonstrate that CD28-CD80 interactions segregate from TCR-MHCp interactions in the IS formed between T cells and CHO cells. Segregation is dependent upon the cytoplasmic domain of CD80 and correlates with enhanced costimulatory activity.
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Altered TCR signaling from geometrically repatterned immunological synapses
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The authors use nano-patterned planar bilayers to demonstrate that the diffusion barriers in the APC membrane can alter the pattern of TCR clusters in the immunological synapse. When cSMAC formation was prevented, signaling was enhanced.
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Mossman K.D., Campi G., Groves J.T., and Dustin M.L. Altered TCR signaling from geometrically repatterned immunological synapses. Science 310 (2005) 1191-1193. The authors use nano-patterned planar bilayers to demonstrate that the diffusion barriers in the APC membrane can alter the pattern of TCR clusters in the immunological synapse. When cSMAC formation was prevented, signaling was enhanced.
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Structural basis for co-stimulation by the human CTLA-4/B7-2 complex
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Crystal structure of a soluble CD28-Fab complex
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Cutting edge: hierarchy of chemokine receptor and TCR signals regulating T cell migration and proliferation
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Bromley S.K., Peterson D.A., Gunn M.D., and Dustin M.L. Cutting edge: hierarchy of chemokine receptor and TCR signals regulating T cell migration and proliferation. J Immunol 165 (2000) 15-19
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