Subtype specificity of scorpion β-toxin Tz1 interaction with voltage-gated sodium channels is determined by the pore loop of domain 3

Molecular Pharmacology

Volumn 70, Issue 1, 2006, Pages 340-347

  Leipold, Enrico ^a   Hansel, Alfred ^a   Borges, Adolfo ^b   Heinemann, Stefan H ^a  

^a FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

^b UNIVERSIDAD CENTRAL DE VENEZUELA   (Venezuela)

Author keywords

[No Author keywords available]

Indexed keywords

EPITOPE; SCORPION VENOM; TZ1 TOXIN; UNCLASSIFIED DRUG; VOLTAGE GATED SODIUM CHANNEL;

ARTICLE; CHIMERA; CONTROLLED STUDY; DRUG SPECIFICITY; HUMAN; HUMAN CELL; NERVE CELL; NUCLEOTIDE SEQUENCE; PERIPHERAL NERVE; PHENOTYPE; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN INTERACTION; SKELETAL MUSCLE; WILD TYPE;

AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; CELL LINE; HUMANS; ION CHANNEL GATING; MEMBRANE POTENTIALS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; PATCH-CLAMP TECHNIQUES; PROTEIN ISOFORMS; SCORPION VENOMS; SEQUENCE HOMOLOGY, AMINO ACID; SODIUM CHANNELS; TRANSFECTION;

EID: 33745228127     PISSN: 0026895X     EISSN: 15210111     Source Type: Journal    
DOI: 10.1124/mol.106.024034     Document Type: Article

References (25)

1. Borges A, Alfonzo MJ, Garcia CC, Winand NJ, Leipold E, and Heinemann SH (2004) Isolation, molecular cloning and functional characterization of a novel β-toxin from the Venezuelan scorpion, Tityus zulianus. Toxicon 43:671-684.
2. Bosmans F, Martin-Eauclaire MF, and Tytgat J (2005) The depressant scorpion neurotoxin LqqIT2 selectively modulates the insect voltage-gated sodium channel. Toxicon 45:501-507.
3. Catterall WA (1992) Cellular and molecular biology of voltage-gated sodium channels. Physiol Rev 72:15-48.
4. Cestèle S, Qu Y, Rogers JC, Rochat H, Scheuer T, and Catterall WA (1998) Voltage sensor-trapping: Enhanced activation of sodium channels by β-scorpion toxin bound to the S3-S4 loop in domain II. Neuron 21:919-931.
5. Cestèle S, Scheuer T, Mantegazza M, Rochat H, and Catterall WA (2001) Neutralization of gating charges in domain II of the sodium channel α subunit enhances voltage-sensor trapping by a β-scorpion toxin. J Gen Physiol 118:291-301.
6. Chen H, Gordon D, and Heinemann SH (1999) Modulation of cloned skeletal muscle sodium channels by the scorpion toxins Lqh II, Lqh III and LqhαIT. Pflueg Arch Eur J Physiol 439:423-432.
7. + channels. J Biol Chem 279:8206-8211.
8. Gellens ME, George AL Jr, Chen LQ, Chahine M, Horn R, Barchi RL, and Kallen RG (1992) Primary structure and functional expression of the human cardiac tetrodotoxin-insensitive voltage-dependent sodium channel. Proc Natl Acad Sci USA 89:554-558.
9. Gordon D, Moskowitz H, Eitan M, Warner C, Catterall WA, and Zlotkin E (1992) Localization of receptor sites for insect-selective toxins on sodium channels by site directed antibodies. Biochemistry 31:7622-7628.
10. Gordon D, Savarin P, Gurevitz M, and Zinn-Justin S (1998) Functional anatomy of scorpion toxins affecting sodium channels. J Toxicol Toxin Rev 17:131-159.
11. Janiszewski L (1990) The action of toxins on voltage-gated sodium channels. Pol J Pharmacol Pharm 42:581-588.
12. Jurman ME, Boland LM, Liu Y, and Yellen G (1994) Visual identification of individual transfected cells for electrophysiology using antibody-coated beads. Biotechniques 17:876-881.
13. Klugbauer N, Lacinova L, Flockerzi V, and Hofmann F (1995) Structure and functional expression of a new member of the tetrodotoxin-sensitive voltage-activated sodium channel family from human neuroendocrine cells. EMBO (Eur Mol Biol Organ) J 14:1084-1090.
14. Kohrman DC, Smith MR, Goldin AL, Harris J, and Meisler MH (1996) A missense mutation in the sodium channel Scn8a is responsible for cerebellar ataxia in the mouse mutant jolting. J Neurosci 16:5993-5999.
15. + channel. Science (Wash DC) 309:897-903.
16. Mantegazza M and Cestèle S (2005) β-Scorpion toxin effects suggest electrostatic interactions in domain II of voltage-dependent sodium channels. J Physiol 568:13-30.
17. Martin-Eauclaire MF and Couraud F (1995) Scorpion neurotoxins: effects and mechanisms, in Handbook of Neurotoxicology (Chang LW and Dyer RS eds) pp 683-716, Marcel Dekker, New York.
18. Massensini AR, Suckling J, Brammer MJ, Moraes-Santos T, Gomez MV, and Romano-Silva MA (2002) Tracking sodium channels in live cells: confocal imaging using fluorescently labeled toxins. J Neurosci Methods 116:189-196.
19. Noda M, Ikeda T, Suzuki H, Takeshima H, Takahashi T, Kuno M, and Numa S (1986) Expression of functional sodium channels from cloned cDNA. Nature (Lond) 322:826-828.
20. +-channels. Eur J Biochem 264:287-300.
21. Shichor I, Zlotkin E, Ilan N, Chikashvili D, Stühmer W, Gordon D, and Lotan I (2002) Domain 2 of Drosophila para voltage-gated sodium channel confers insect properties to a rat brain channel. J Neurosci 22:4364-4371.
22. Sternberg D, Maisonobe T, Jurkat-Rott K, Nicole S, Launay E, Chauveau D, Tabti N, Lehmann-Horn F, and Hainque B (2001) Hypokalaemic periodic paralysis type 2 caused by mutations at codon 672 in the muscle sodium channel gene SCN4A. Brain 124:1091-1099.
23. Trimmer JS, Cooperman SS, Tomiko SA, Zhou JY, Crean SM, Boyle MB, Kallen RG, Sheng ZH, Barchi RL, Sigworth FJ, et al. (1989) Primary structure and functional expression of a mammalian skeletal muscle sodium channel. Neuron 3:33-49.
24. Zorn S, Leipold E, Hansel A, Bulaj G, Olivera BM, Terlau H, and Heinemann SH (2006) The μO-conotoxin MrVIA inhibits voltage-gated sodium channels by associating with domain 3. FEBS Lett 580:1360-1364.
25. Zuo XP and Ji YH (2004) Molecular mechanism of scorpion neurotoxins acting on sodium channels. Mol Neurobiol 30:265-278.