Molecular cloning of the Leishmania major UDP-glucose pyrophosphorylase, functional characterization, and ligand binding analyses using NMR spectroscopy

Journal of Biological Chemistry

Volumn 281, Issue 24, 2006, Pages 16314-16322

  Lamerz, Anne Christin ^a   Haselhorst, Thomas ^b   Bergfeld, Anne K ^a   Von Itzstein, Mark ^b   Gerardy Schahn, Rita ^a  

^a HANNOVER MEDICAL SCHOOL   (Germany)

^b GRIFFITH UNIVERSITY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

BIOSYNTHESIS; CLONING; ENZYME KINETICS; GENES; GLUCOSE; MICROORGANISMS; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; OLIGOMERS; POLYMERIZATION; PROTOZOA;

GLUCOSE MOIETY; MOLECULAR CLONING; SATURATION TRANSFER DIFFERENCE (STD); UDP-GLUCOSE PYROPHOSPHORYLASE (UGP);

ENZYMES;

GALACTOSE 1 PHOSPHATE; GLUCOSE 1 PHOSPHATE; LIGAND; MONOMER; NUCLEOTIDE; RECOMBINANT ENZYME; UNCLASSIFIED DRUG; URIDINE DIPHOSPHATE GALACTOSE; URIDINE DIPHOSPHATE GLUCOSE; URIDINE TRIPHOSPHATE; URIDINE TRIPHOSPHATE GLUCOSE 1 PHOSPHATE URIDYLYLTRANSFERASE;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DONOR; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME CONFORMATION; ENZYME MECHANISM; ENZYME SPECIFICITY; EPIMERIZATION; EUKARYOTE; GEL PERMEATION CHROMATOGRAPHY; GENETIC COMPLEMENTATION; GENETIC ORGANIZATION; GEOMETRY; GLUCOSE METABOLISM; HAMSTER; LEISHMANIA MAJOR; LIGAND BINDING; MICHAELIS CONSTANT; MIGRATION; MOLECULAR CLONING; MOLECULAR MECHANICS; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN PURIFICATION; REGULATORY MECHANISM;

ANIMALS; CHO CELLS; CLONING, MOLECULAR; CRICETINAE; CYTOSOL; GENETIC COMPLEMENTATION TEST; GLUCOSE; HUMANS; KINETICS; LEISHMANIA MAJOR; MODELS, BIOLOGICAL; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; UTP-GLUCOSE-1-PHOSPHATE URIDYLYLTRANSFERASE;

EUKARYOTA; LEISHMANIA MAJOR; MAMMALIA; PROTOZOA;

EID: 33745216038     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M600076200     Document Type: Article

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