Sequence differences between human muscle and liver cDNAs for UDPglucose pyrophosphorylase and kinetic properties of the recombinant enzymes expressed in Escherichia coli

European Journal of Biochemistry

Volumn 235, Issue 1-2, 1996, Pages 173-179

  Duggleby, Ronald G ^a   Chao, Yu Chyi ^b   Huang, Joyce G ^b   Peng, Hwei Ling ^b   Chang, Hwan You ^b  

^a UNIVERSITY OF QUEENSLAND   (Australia)

^b CHANG GUNG UNIVERSITY   (Taiwan)

Author keywords

cDNA sequence; enzyme kinetics; galU; human isoenzymes; UDPglucose pyrophosphorylase

Indexed keywords

ISOENZYME; URIDINE TRIPHOSPHATE GLUCOSE 1 PHOSPHATE URIDYLYLTRANSFERASE;

ARTICLE; DNA LIBRARY; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE EXPRESSION; HUMAN; HUMAN TISSUE; LIVER; MUSCLE; PRIORITY JOURNAL; SEQUENCE ANALYSIS;

ESCHERICHIA COLI;

EID: 0030042361     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1996.00173.x     Document Type: Article

References (14)

1. Aksamit, R. R. & Ebner, K. E. (1972) Purification, properties and kinetic analysis of UDP-glucose pyrophosphorylase from bovine mammary tissue, Biochim. Biophys. Acta 268, 102-112.
2. Cullmann, G., Hubscher, U. & Berchtold, M. W. (1993) A reliable protocol for dsDNA and PCR product sequencing, BioTechniques 14, 578-579.
3. Duggleby, R. G. (1984) Regression analysis of nonlinear Arrhenius plots: an empirical model and a computer program, Comput. Biol. Med. 14, 447-455.
4. Duggleby, R. G. (1990) Pooling and comparing estimates from several experiments of a Michaelis constant for an enzyme, Anal. Biochem. 189, 84-87.
5. Knop, J. K. & Hansen, R. G. (1970) Uridine diphosphate glucose pyrophosphorylase. IV. crystallization and properties of the enzyme from human liver, J. Biol. Chem. 245, 2499-2504.
6. Levine, S., Gillett, T. A., Hageman, E. & Hansen, R. G. (1969) Uridine diphosphate glucose pyrophosphorylase. II. Polymeric and subunit structure, J. Biol. Chem. 244, 5729-5734.
7. Peng, H.-L. & Chang, H.-Y. (1993) Cloning of a human liver UDP-glucose pyrophosphorylase cDNA by complementation of the bacterial galU mutation, FEBS Lett. 329, 153-158.
8. Putt, W., Ives, J. H., Hollyoake, M., Hopkinson, D. A., Whitehouse, D. B. & Edwards, Y. H. (1993) Phosphoglucomutase 1: a gene with two promotors and a duplicated first exon, Biochem. J. 296, 417-422.
9. 2) to chromosome 2 in man, Cytogenet. Cell Genet. 22, 215-218.
10. Steelman, V. S. & Ebner, K. E. (1966) The enzymes of lactose biosynthesis. I. Purification and properties of UDPG pyrophosphorylase from bovine mammary tissue, Biochim. Biophys. Acta 128, 92-99.
11. Tsuboi, K. K., Fukunaga, K. & Petricciani, J. C. (1969) Purification and specific kinetic properties of erythrocyte uridine diphosphate glucose pyrophosphorylase, J. Biol. Chem. 244, 1008-1015.
12. Turnquist, R. L. & Hansen, R. G. (1973) Uridine diphosphoryl glucose pyrophosphorylase, in The enzymes (Boyer, P. D., ed.) vol. 8, pp. 51-71. Academic Press, New York.
13. van Someren, H., Beijersbergen van Henegouwen, H., Westerveld, A. & Bootsma, D. (1974) Synteny of the human loci for fumarate hydratase and UDPG pyrophosphorylase with chromosome 1 markers in somatic cell hybrids, Cytogenet. Cell Genet. 13, 551-557.
14. Villar-Palasi, C. & Lamer, J. (1960) Uridinediphosphate glucose pyrophosphorylase from skeletal muscle, Arch. Biochem. Biophys. 86, 61-66.