Genotypic characterization of Helicobacter pylori cagA and vacA from biopsy specimens of patients with gastroduodenal diseases

Mount Sinai Journal of Medicine

Volumn 73, Issue 3, 2006, Pages 622-626

  Chang, Yih Hsin ^a   Wang, Lina ^a   Lee, Ming Shih ^a,b   Cheng, Chun Wen ^c   Wu, Chun Ying ^d   Shiau, Ming Yuh ^d  

^a School of Medical Laboratory and Biotechnology   (Taiwan)

^b TAICHUNG VETERANS GENERAL HOSPITAL   (Taiwan)

^c CHUNG SHAN MEDICAL UNIVERSITY   (Taiwan)

^d HUNGKUANG UNIVERSITY   (Taiwan)

Author keywords

cagA; Gastroduodenal diseases; Helicobacter pylori; vacA

Indexed keywords

CAGA PROTEIN; GENOMIC DNA; RNA 16S; VACUOLATING TOXIN;

ADOLESCENT; ADULT; AGED; ARTICLE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; CONTROLLED STUDY; CORRELATION ANALYSIS; DISEASE ASSOCIATION; DUODENUM ULCER; FEMALE; GASTROENTERITIS; GASTROINTESTINAL BIOPSY; GENE SEQUENCE; GENOTYPE; HELICOBACTER INFECTION; HELICOBACTER PYLORI; HUMAN; HUMAN TISSUE; MAJOR CLINICAL STUDY; MALE; NONHUMAN; POLYMERASE CHAIN REACTION; POPULATION DISTRIBUTION; SEQUENCE ANALYSIS; STATISTICAL ANALYSIS; STATISTICAL SIGNIFICANCE; STOMACH ULCER; TAIWAN;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; ANTIGENS, BACTERIAL; BACTERIAL PROTEINS; BIOPSY; DUODENAL DISEASES; FEMALE; GENOTYPE; HELICOBACTER INFECTIONS; HELICOBACTER PYLORI; HUMANS; MALE; MIDDLE AGED; PREVALENCE; STOMACH DISEASES; TAIWAN;

EID: 33744823507     PISSN: 00272507     EISSN: 00272507     Source Type: Journal    
DOI: None     Document Type: Article

References (28)

1. Brown LM. Helicobacter pylori: epidemiology and routes of transmission. Epidemiol Rev 2000; 22:283-297.
2. Goodman KJ, Cockburn M. The role of epidemiology in understanding the health effects of Helicobacter pylori. Epidemiology 2001; 12(2):266-271.
3. Taylor DN, Blaser MJ. The epidemiology of Helicobacter pylori infection. Epidemiol Rev 1991; 13:42-59.
4. Fiedorek SC, Malaty HM, Evans DL, et al. Factors influencing epidemiology of Helicobacter pylori infection in children. Pediatrics 1991; 88:578-582.
5. Marshall BJ. Helicobacter pylori. Am J Gastroenterol 1994; 89: (8 Suppl):S116-S128.
6. Cover TL. The vacuolating cytotoxin of Helicobacter pylori. Mol Microbiol 1996; 20:214-246.
7. Rota CA, Pepeira-Lima JC, Blaya C, Nardi NB. Consensus and variable region PCR analysis of Helicobacter pylori 3′ region of cagA gene in isolates from individuals with or without peptic ulcer. J Clin Microbiol 2001; 39:606-612.
8. Maeda S, Kanai F, Ogura K, et al. High seropositivity of anti-CagA antibody in Helicobacter pylori-infected patients irrelevant to peptic ulcers and normal mucosa in Japan. Dig Dis Sci 1997; 42:1841-1847.
9. Pan ZJ, Berg DE, van der Hulst RW, et al. Prevalence of vacuolating cytotoxin production and distribution of distinct vacA alleles in Helicobacter pylori from China. J Infect Dis 1998; 178:220-226.
10. Shyu RY, Jiang ST, Lai CH, et al. High frequency of cytotoxin-associated gene A in Helicobacter pylori isolated from asymptomatic subjects and peptic ulcer patients in Taiwan. J Clin Gastroenterol 1998; 27:54-59.
11. Yamaoka Y, Kodama T, Kashima K, et al. Variants of the 3′ region of the cagA gene in Helicobacter pylori isolates from patients with different H. pylori-associated diseases. J Clin Microbiol 1998; 36(8):2258-2263.
12. Leunk RD, Johnson PT, David BC, et al. Cytotoxic activity in broth-culture filtrates of Campylobacter pylori. J Med Microbiol 1988; 26:93-99.
13. Atherton JC, Cao P, Peek RM, et al. Mosaicism in vacuolating cytotoxin alleles of Helicobacter pylori. Association of specific vacA type with cytotoxin production and peptic ulceration. J Biol Chem 1995; 270:17771-17777.
14. Cover TL, Tummuru MK, Cao P, et al. Divergence of genetic sequences for the vacuolating cytotoxin among Helicobacter pylori strains. J Biol Chem 1994; 269:10566-10573.
15. Atherton JC, Peek RM, Tham KT, et al. Clinical and pathological importance of heterogeneity in vacA, the vacuolating cytotoxin gene of Helicobacter pylori. Gastroenterology 1997; 112:92-99.
16. Basso D, Navaglia F, Brigato L, et al. Analysis of Helicobacter pylori vacA and cagA genotypes and serum antibody profile in benign and malignant gastroduodenal disease. Gut 1998; 43:182-186.
17. Warburton VJ, Everett S, Mapstone NP, et al. Clinical and histological association of cagA and vacA genotypes in Helicobacter pylori gastritis. J Clin Pathol 1998; 51:55-61.
18. van Doorn LJ, Figueiredo C, Megraud F, et al. Geographic distribution of vacA allelic types of Helicobacter pylori. Gastroenterology 1999; 116(4):823-830.
19. Kimura M, Goto S, Wada A, et al. Vacuolating cytotoxin purified from Helicobacter pylori causes mitochrondrial damage in human gastric cells. Microb Pathog 1999; 26(1):45-52.
20. Pan ZJ, van der Hulst RW, Feller M, et al. Equally high prevalence of infection with cagA positive Helicobacter pylori in Chinese patients with peptic ulcer disease and those with chronic gastritis-associated dyspepsia. J Clin Microbiol 1997; 35:1344-1347.
21. Wang HJ, Kuo CH, Yeh AA, et al. Vacuolating toxin production in clinical isolates of Helicobacter pylori with different vacA genotypes. J Infect Dis 1998; 178:207-212.
22. Yang JC, Kuo CH, Wang HJ, et al. Vacuolating toxin gene polymorphism among Helicobacter pylori clinical isolates and its association with m1, m2, or chimeric vacA middle types. Scand J Gastroenterol 1998; 33:1152-1157.
23. Zhang Y, Liu H, Zhou K. Lack of correlation of vacA genotype, cagA gene of Helicobacter pylori and their expression production with various gastroduodenal diseases. J Chin Med 2001; 114:703-706.
24. Tummuru MK, Cover TL, Blaser MJ. Cloning and expression of a high-molecular-mass major antigen of Helicobacter pylori: evidence of linkage to cytotoxin production. Infect Immun 1993; 61:1799-1809.
25. Tummuru MK, Cover TL, Blaser MJ. Mutation of the cytotoxin-associated cagA gene does not affect the vacuolating cytotoxin activity of Helicobacter pylori. Infect Immun 1994; 62:2609-2613.
26. Xiang Z, Censini S, Bayeli PF, et al. Analysis of expression of CagA and VacA virulence factors in 43 strains of Helicobacter pylori reveals that clinical isolates can be divided into two major types and that CagA is not necessary for expression of the vacuolating cytotoxin. Infect Immun 1995; 63:94-98.
27. Huang J, Doig P, Trust TJ. Stimulation of interleukin-8 production in epithelial cell lines by Helicobacter pylori. Infect Immun 1995; 63:1732-1738.
28. Sharma SA, Tummuru MK, Miller GG, Blaser MJ. Interleukin-8 response of gastric epithelial cell lines to Helicobacter pylori stimulation in vitro. Infect Immun 1995; 63:1681-1687.