Rapid production of functionalized recombinant proteins: Marrying ligation independent cloning and in vitro protein ligation

Bioconjugate Chemistry

Volumn 17, Issue 3, 2006, Pages 610-617

  Kushnir, Susanna ^a   Marsac, Yoann ^a   Breitling, Reinhard ^b   Granovsky, Igor ^c   Brok Volchanskaya, Vera ^c   Goody, Roger S ^a   Becker, Christian F W ^a   Alexandrov, Kirill ^a,b  

^a MAX PLANCK INSTITUTE OF MOLECULAR PHYSIOLOGY   (Germany)

^b Jena Bioscience GmbH   (Germany)

^c SKRYABIN INSTITUTE OF BIOCHEMISTRY AND PHYSIOLOGY OF MICROORGANISMS   (Russian Federation)

Author keywords

[No Author keywords available]

Indexed keywords

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; CLONE CELLS; CLONING; ESCHERICHIA COLI; GENES; MAMMALS; MOLECULAR BIOLOGY; PURIFICATION; RECOMBINANT PROTEINS;

ACTIVE FIELD; CODING GENES; CYSTEINE RESIDUES; FUNCTIONAL GENOMICS; FUNCTIONALIZED; IN-VITRO; N-TERMINALS; PHYSIOLOGICAL ROLES; PROTEIN LIGATION; TERMINAL CYSTEINE;

VECTORS;

BIOTIN; CYSTEINE; FLUORESCEIN; GLUTATHIONE; GUANOSINE TRIPHOSPHATASE; PROTEINASE; RAB PROTEIN; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; AMINO TERMINAL SEQUENCE; ARTICLE; COVALENT BOND; ESCHERICHIA COLI; EXPRESSION VECTOR; FUNCTIONAL GENOMICS; FUNCTIONAL PROTEOMICS; GENE SEQUENCE; MAMMAL; MOLECULAR CLONING; PROTEIN DEGRADATION; PROTEIN MODIFICATION; PROTEIN PURIFICATION; PROTEIN TARGETING; TECHNIQUE;

ESCHERICHIA COLI; MAMMALIA; TOBACCO VEIN MOTTLING VIRUS;

EID: 33646899308     PISSN: 10431802     EISSN: None     Source Type: Journal    
DOI: 10.1021/bc050320d     Document Type: Article

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