Direct production of proteins with N-terminal cysteine for site-specific conjugation

Bioconjugate Chemistry

Volumn 15, Issue 3, 2004, Pages 658-663

  Gentle, Ian E ^a   De Souza, David P ^a   Baca, Manuel ^a,b  

^a WALTER AND ELIZA HALL INSTITUTE OF MEDICAL RESEARCH   (Australia)

^b Zenyth Therapeutics Limited Australia   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CHEMICAL MODIFICATION; ESCHERICHIA COLI;

DIRECT PRODUCTION; FUSION PROTEINS; LABELINGS; N-TERMINALS; NATIVE CHEMICAL LIGATION; SEMISYNTHESIS; SITE-SPECIFIC; SPECIFIC CLEAVAGES; SPECIFIC CONJUGATIONS; TERMINAL CYSTEINE;

RECOMBINANT PROTEINS;

CYSTEINE; FLUORESCEIN; HYBRID PROTEIN; METHIONINE; METHIONYL AMINOPEPTIDASE; RECOMBINANT PROTEIN; THIOESTER;

AMINO ACID COMPOSITION; AMINO TERMINAL SEQUENCE; ARTICLE; CONJUGATION; ESCHERICHIA COLI; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN MODIFICATION; PROTEIN SYNTHESIS; TECHNIQUE; TECHNOLOGY;

ESCHERICHIA COLI;

EID: 2442688260     PISSN: 10431802     EISSN: None     Source Type: Journal    
DOI: 10.1021/bc049965o     Document Type: Article

References (19)

1. Gaertner, H. F., Rose, K., Cotton, R., Timms, D., Camble, R., and Offord, R. E. (1992) Construction of protein analogues by site-specific condensation of unprotected fragments. Bioconjugate Chem. 3, 262-268.
2. Dawson, P. E., Muir, T. W., Clark-Lewis, I., and Kent, S. B. H. (1994) Synthesis of proteins by native chemical ligation. Science 266, 776-779.
3. Liu, C. F., and Tam, J. P. (1994) Peptide segment ligation strategy without use of protecting groups. Proc. Natl. Acad. Sci. U.S.A. 91, 6584-6588.
4. Erlanson, D. A., Chytil, M., and Verdine, G. L. (1996) The leucine zipper domain controls the orientation of AP-1 in the NFAT. AP-1.DNA complex. Chem. Biol. 3, 981-991.
5. Kapanidis, A. N., and Weiss, S. (2002) Fluorescent probes and bioconjugation chemistries for single-molecule fluorescence analysis of biomolecules J. Chem. Phys. 117, 10953-10964.
6. Pal, G., Santamaria, F., Kossiakoff, A. A., and Lu, W. Y. (2003) The first semi-synthetic serine protease made by native chemical ligation. Protein Expr. Purif. 29, 185-192.
7. Xu, R., Ayers, B., Cowburn, D., and Muir, T. W. (1999) Chemical ligation of folded recombinant proteins: segmental isotopic labeling of domains for NMR studies. Proc. Natl. Acad. Sci. U.S.A. 96, 388-393.
8. Dawson, P. E., and Kent, S. B. H. (2000) Synthesis of native proteins by chemical ligation. Annu. Rev. Biochem. 69, 923-960.
9. Tolbert, T. J., and Wong, C. H. (2002) New methods for proteomic research: preparation of proteins with N-terminal cysteines for labeling and conjugation. Angew. Chem., Int. Ed. 41, 2171-2174.
10. Sherman, F., Stewart, J. W., and Tsunasawa, S. (1985) Methionine or not methionine at the beginning of a protein. Bioessays 3, 27-31.
11. Wilson, D. B., and Dintzis, H. M. (1970) Protein chain initiation in rabbit reticulocytes. Proc. Natl. Acad. Sci. U.S.A. 66, 1282-1289.
12. Yoshida, A., Watanabe, S., and Morris, J. (1970) Initiation of rabbit hemoglobin synthesis: methionine and formylmethionine at the N-terminal. Proc. Natl. Acad. Sci. U.S.A. 67, 1600-1607.
13. Jackson, R., and Hunter, T. (1970) Role of methionine in the initiation of haemoglobin synthesis. Nature 227, 672-675.
14. Boissel, J. P., T. J., K., and Bunn, H. F. (1988) Cotranslational amino-terminal processing of cytosolic proteins. Cell-free expression of site-directed mutants of human hemoglobin. J. Biol. Chem. 263, 8443-8449.
15. Hirel, P. H., Schmitter, M. J., Dessen, P., Fayat, G., and Blanquet, S. (1989) Extent of N-terminal methionine excision from Escherichia coli proteins is governed by the side-chain length of the penultimate amino acid. Proc. Natl. Acad. Sci. U.S.A. 86, 8247-8251.
16. Huang, S., Elliott R. C., Liu P. S., Koduri R. K., Weickmann J. L., Lee J. H., Blair L. C., Ghosh-Dastidar P., Bradshaw R. A., Bryan K. M., Einarson, B., Kendall, R. L., Kolacs, K. H., and Saito, K. (1987) Specificity of cotranslational amino-terminal processing of proteins in yeast. Biochemistry 26, 8242-8246.
17. Iwai, H., and Pluckthun, A. (1999) Circular beta-lactamase: stability enhancement by cyclizing the backbone. FEBS Lett. 459, 166-172.
18. De Souza, D., Fabri, L. J., Nash, A., Hilton, D. J., Nicola, N. A., and Baca, M. (2002) SH2 domains from suppressor of cytokine signaling-3 and protein tyrosine phosphatase SHP-2 have similar binding specificities. Biochemistry 41, 9229-9236.
19. Villain, M., Vizzavona, J., and Rose, K. (2001) Covalent capture: a new tool for the purification of synthetic and recombinant polypeptides. Chem. Biol. 8, 673-679.