A sensitive fluorescence monitor for the detection of activated Ras: Total chemical synthesis of site-specifically labeled Ras binding domain of c-Raf1 immobilized on a surface

Chemistry and Biology

Volumn 8, Issue 3, 2001, Pages 243-252

  Becker, Christian F W ^a   Hunter, Christie L ^b   Seidel, Ralf P ^a   Kent, Stephen B H ^b   Goody, Roger S ^a   Engelhard, Martin ^a  

^a MAX PLANCK INSTITUTE OF MOLECULAR PHYSIOLOGY   (Germany)

^b Gryphon Therapeutics   (United States)

Author keywords

Chemical protein synthesis; Fluorescent biosensor; Native chemical ligation; Ras binding domain of c Raf1; Site specific labeling

Indexed keywords

BIOSENSOR; CARBOXY TERMINAL SEQUENCE; EUKARYOTIC CELL; FLUORESCENCE; GENE ACTIVATION; GENE TAGGING; GUANINE NUCLEOTIDE BINDING PROTEIN; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN PHOSPHORYLATION; PROTEIN SYNTHESIS; RAS PROTEIN; SIGNAL TRANSDUCTION;

AFFINITY LABELS; ANIMALS; BINDING SITES; BIOSENSING TECHNIQUES; FLUORESCENT DYES; GUANOSINE TRIPHOSPHATE; HUMANS; PEPTIDE FRAGMENTS; PROTO-ONCOGENE PROTEINS C-RAF; RAS PROTEINS; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, FLUORESCENCE;

EUKARYOTA;

EID: 0035071606     PISSN: 10745521     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1074-5521(01)00003-5     Document Type: Article

References (36)

1. Synthesis of proteins by native chemical ligation
2. Synthesis of native proteins by chemical ligation
3. Total chemical synthesis of enzymatically active human type II secretory phospholipase A2
4. 1-methyl-7-azaTrp]Ras-binding domain of c-Raf1
5. Total chemical synthesis of the integral membrane protein influenza A virus M2 proton channel: Role of its cytoplasmic domain for pore assembly
6. Expressed protein ligation: A general method for protein engineering
7. Chemical protein synthesis
8. Generation of a dual-labeled fluorescence biosensor for Crk-II phosphorylation using solid-phase expressed protein ligation
9. Enzyme-based biosensors for in vivo measurements
10. Biochips
11. Analysis of yeast protein kinases using protein chips
12. Printing proteins as microarrays for high-throughput function determination
13. Ras target proteins in eukaryotic cells
14. Guanosine triphosphatase stimulation of oncogenic Ras mutants
15. Ras genes
16. Mammalian Ras interacts directly with the serine/threonine kinase Raf
17. The 2.2 Å crystal structure of the Ras-binding domain of the serine/threonine kinase c-Raf1 in complex with Rap1A and a GTP analogue
18. Quantitative analysis of the complex between p21(Ras) and the Ras-binding domain of the human RAF-1 protein kinase
19. Transient kinetic studies on the interaction of Ras and the Ras-binding domain of c-Raf-1 reveal rapid equilibration of the complex
20. Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus
21. How strong is the coordinate bond between a histidine tag and Ni-nitrilotriacetate? An experiment of mechanochemistry on single molecules
22. Characterization of a 78-residue fragment of c-Raf-1 that comprises a minimal binding domain for the interaction with Ras-GTP
23. Quantitative Structure-activity analysis correlating Ras/RAF Interaction in vitro to RAF activation in vivo
24. L-(6,7-Dimethoxy-4-coumaryl) alanine: An intrinsic probe for the labelling of peptides
25. Synthesis and properties of (6,7-dimethoxy-4-coumaryl)alanine: A fluorescent peptide label
26. Probing the structure and function of the tachykinin neurokinin-2 receptor through biosynthetic incorporation of fluorescent amino acids at specific sites
27. A new tool for studying protein structure and function
28. Semi-synthetic Rab proteins as tools for studying intermolecular interactions
29. Facile liquid chromatographic enantioresolution of native amino acids and peptides using a Teicoplanin chiral stationary phase
30. High-performance liquid chromatography separation of enantiomers of unusual amino acids on a Teicoplanin chiral stationary phase
31. In situ neutralization in Boc-chemistry solid phase peptide synthesis
32. Solid phase synthesis
33. Development of a linker with an enhanced stability for the preparation of peptide thioesters and its application to the synthesis of a stable-isotope-labeled HU-type DNA-binding protein
34. Modulation of reactivity in native chemical ligation through the use of thiol additives
35. Expression of p21 proteins in Escherichia coli and stereochemistry of the nucleotide-binding site
36. Kinetics of interaction of nucleotides with nucleotide-free H-ras p21