Expression, refolding, and characterization of GFE Peptide-Fused Human Interferon-α2a in Escherichia coli

Applied Biochemistry and Biotechnology

Volumn 133, Issue 2, 2006, Pages 149-162

  Yan, Zhen ^a,b   Lu, Li ^c   Shi, Jihong ^a,b   Bao, Chunjie ^a,b   Han, Wei ^a,b   Wu, Yongjie ^c   Zhang, Yingqi ^a,b  

^a FOURTH MILITARY MEDICAL UNIVERSITY   (China)

^b State Key Laboratory of Cancer Biology   (China)

^c LANZHOU UNIVERSITY   (China)

Author keywords

DNA recombination; Expression; Interferon 2a; Purification; Target peptide; Tissue distribution

Indexed keywords

DNA RECOMBINATION; EXPRESSION; INTERFERON; TARGET PEPTIDE; TISSUE DISTRIBUTION;

BIOASSAY; CHROMATOGRAPHY; DIALYSIS; DNA; ELECTROPHORESIS; ENZYMES; OLIGOMERS; POLYACRYLATES; POLYPEPTIDES; PURIFICATION; TISSUE CULTURE; VIRUSES;

ESCHERICHIA COLI;

ALPHA2A INTERFERON; CYSTEINYLGLYCYLPHENYLALANYLGLUTAMYLCYSTEINYLVALYLARGINYLGLUTAMINYL CYSTEINYLPROLYLGLUTAMYLARGINYLCYSTEINE; HYBRID PROTEIN; LUNG HOMING OLIGOPEPTIDE; PEPTIDE; RNA POLYMERASE; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIVIRAL ACTIVITY; ARTICLE; CELL INCLUSION; CONTROLLED STUDY; CULTURE MEDIUM; DIALYSIS; ESCHERICHIA COLI; FEMALE; FUSION GENE; IMMUNOBLOTTING; IN VITRO STUDY; KIDNEY; LIVER; LUNG; MALE; NONHUMAN; PHAGE DISPLAY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION;

ANIMALS; ANTIVIRAL AGENTS; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; HUMANS; INTERFERON ALFA-2A; KIDNEY; LUNG; MICE; OLIGOPEPTIDES; PROTEIN BINDING; PROTEIN FOLDING; RECOMBINANT FUSION PROTEINS;

ANIMALIA; ESCHERICHIA COLI;

EID: 33646876609     PISSN: 02732289     EISSN: None     Source Type: Journal    
DOI: 10.1385/ABAB:133:2:149     Document Type: Article

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