Direct transfer of membrane proteins from bacteria to planar bilayers for rapid screening by single-channel recording

Nature Chemical Biology

Volumn 2, Issue 6, 2006, Pages 314-318

  Holden, Matthew A ^a   Jayasinghe, Lakmal ^a   Daltrop, Oliver ^a   Mason, Amy ^a   Bayley, Hagan ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; BACTERIAL TOXIN; LEUKOCIDIN; MEMBRANE PROTEIN; POTASSIUM CHANNEL; PROTEIN SUBUNIT;

ALPHA HELIX; ARTICLE; BACTERIUM COLONY; BILAYER MEMBRANE; ESCHERICHIA COLI; LIPID BILAYER; MEMBRANE POTENTIAL; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN STRUCTURE; PROTEIN TRANSPORT; PROTEOMICS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 33646869929     PISSN: 15524450     EISSN: 15524469     Source Type: Journal    
DOI: 10.1038/nchembio793     Document Type: Article

References (23)

1. Miller,, C. Ion channel reconstitution (Plenum, New York, 1986).
2. Holden, M.A. & Bayley, H. Direct introduction of single protein channels and pores into lipid bilayers. J. Am. Chem. Soc. 127, 6502-6503 (2005).
3. + conduction and selectivity. Science 280, 69-77 (1998).
4. + channels: functional asymmetries and sidedness of proton activation. J. Gen. Physiol. 114, 551-560 (1999).
5. + channel. J. Gen. Physiol. 111, 741-749 (1998).
6. + channel: tryptophan scanning and complementation analysis lead to mutants with altered gating. Biochemistry 41, 13653-13662 (2002).
7. Miles, G., Cheley, S., Braha, O. & Bayley, H. The staphylococcal leukocidin bi-component toxin forms large ionic channels. Biochemistry 40, 8514-8522 (2001).
8. Bayley, H. & Cremer, P.S. Stochastic sensors inspired by biology. Nature 413, 226-230 (2001).
9. Kang, X.F, Gu, L.Q., Cheley, S. & Bayley, H. Single protein pores containing molecular adapters at high temperatures. Angew. Chem. Int. Edn Engl. 44, 1495-1499 (2005).
10. Montoya, M. & Gouaux, E. β-barrel membrane protein folding and structure viewed through the lens of α-hemolysin. Biochim. Biophys. Acta. 1609, 19-27 (2003).
11. Kaneko, J. & Kamio, Y. Bacterial two-component and hetero-heptameric pore-forming cytolytic toxins: structures, pore-forming mechanism, and organization of the genes. Biosci. Biotechnol. Biochem. 68, 981-1003 (2004).
12. Miles, G., Movileanu, L. & Bayley, H. Subunit composition of a bicomponent toxin: staphylococcal leukocidin forms an octameric transmembrane pore. Protein Sci. 11, 894-902 (2002).
13. Jayasinghe, L. & Bayley, H. The leukocidin pore: evidence for an octamer with four LukF subunits and four LukS subunits alternating around a central axis. Protein Sci. 14, 2550-2561 (2005).
14. Gu, L.-Q., Braha, O., Conlan, S., Cheley, S. & Bayley, H. Stochastic sensing of organic analytes by a pore-forming protein containing a molecular adapter. Nature 398, 686-690 (1999).
15. Gu, L.-Q., Cheley, S. & Bayley, H. Capture of a single molecule in a nanocavity. Science 291, 636-640 (2001).
16. Gu, L.-Q., Cheley, S. & Bayley, H. Prolonged residence time of a noncovalent molecular adapter, β-cyclodextrin, within the lumen of mutant α-hemolysin pores. J. Gen. Physiol. 118, 481-493 (2001).
17. Olson, R., Nariya, H., Yokota, K., Kamio, Y. & Gouaux, E. Crystal structure of staphylococcal LukF delineates conformational changes accompanying formation of a transmembrane channel. Nat. Struct. Biol. 6, 134-140 (1999).
18. Pedelacq, J.D. et al. The structure of a Staphylococcus aureus leukocidin component (LukF-PV) reveals the fold of the water-soluble species of a family of transmembrane pore-forming toxins. Structure 7, 277-287 (1999).
19. Guillet, V. et al. Crystal structure of leucotoxin S component - new insight into the staphylococcal β-barrel pore-forming toxins. J. Biol. Chem. 279, 41028-41037 (2004).
20. Cheley, S. et al. Spontaneous oligomerization of a staphylococcal α-hemolysin conformationally constrained by removal of residues that form the transmembrane β-barrel. Protein Eng. 10, 1433-1443 (1997).
21. Howorka, S. & Bayley, H. Improved protocol for high-throughput cysteine scanning mutagenesis. Biotechniques 25, 764-772 (1998).
22. Braha, O. et al. Designed protein pores as components for biosensors. Chem. Biol. 4, 497-505 (1997).
23. Prevost, G., Mourey, L., Colin, D.A. & Menestrina, G. in Pore-forming Toxins Vol. 257 (ed. van der Goot, G.) 53-83 (Springer, Berlin, 2001).