Prolonged residence time of a noncovalent molecular adapter, β-Cyclodextrin, within the lumen of mutant α-Hemolysin pores

Journal of General Physiology

Volumn 118, Issue 5, 2001, Pages 481-493

  Gu, L Q ^a   Cheley, S ^a   Bayley, H ^a  

^a TEXAS A AND M UNIVERSITY SYSTEM   (United States)

Author keywords

toxin; Molecular adapter; Mutagenesis; Pore; Stochastic sensing

Indexed keywords

BETA CYCLODEXTRIN; HEMOLYSIN;

ARTICLE; BINDING SITE; CHANNEL GATING; ELECTROOSMOSIS; MEMBRANE POTENTIAL; NONHUMAN; SENSOR; SITE DIRECTED MUTAGENESIS; STOCHASTIC MODEL;

BACTERIAL TOXINS; BASE SEQUENCE; BETA-CYCLODEXTRINS; BINDING SITES; CARCINOGENS; CYCLODEXTRINS; HEMOLYSIN PROTEINS; KINETICS; MEMBRANE POTENTIALS; MEMBRANE TRANSPORT PROTEINS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; STAPHYLOCOCCUS AUREUS;

EID: 0035169385     PISSN: 00221295     EISSN: None     Source Type: Journal    
DOI: 10.1085/jgp.118.5.481     Document Type: Article

References (35)

1. Imaging molecular transport in porous membranes. Observation and analysis of electroosmotic flow in individual pores using the scanning electrochemical microscope
2. Electrically facilitated molecular transport. Analysis of the relative contributions of diffusion, migration, and electroosmosis to solute transport in an ion-exchange membrane
3. Pore-forming proteins with built-in triggers and switches
4. Building a door into cells
5. Designed membrane channels and pores
6. Stochastic sensing with protein pores
7. Stochastic sensors inspired by biology
8. Probing sugar translocation through maltoporin at the single channel level
9. Staphylococcal α-toxin: Oligomerization of hydrophilic monomers to form amphiphilic hexamers induced through contact with deoxycholate micelles
10. Designed protein pores as components for biosensors
11. Simultaneous stochastic sensing of divalent metal ions
12. Spontaneous oligomerization of a staphylococcal a-hemolysin conformationally constrained by removal of residues that form the transmembrane b barrel
13. A functional protein pore with a "retro" transmembrane domain
14. Cyclodextrins
15. Intracellular trehalose improves the survival of cryopreserved mammalian cells
16. On the mechanism of membrane damage by Staphylococcus aureus α-toxin
17. Interaction of the non-covalent molecular adapter, β-cyclodextrin, with the staphylococcal α-hemolysin pore
18. Stochastic sensing of organic analytes by a pore-forming protein containing a molecular adapter
19. Reversal of charge selectivity in transmembrane protein pores by using non-covalent molecular adapters
20. Capture of a single molecule in a nanocavity
21. Sequence-specific detection of individual DNA strands using engineered nanopores
22. Kinetics of duplex formation for individual DNA strands within a single protein nanopore
23. Characterization of individual polynucleotide molecules using a membrane channel
24. Influence of Cys-130 S. aureus alpha-toxin on planar lipid bilayer and erythrocyte membranes
25. Formation of bimolecular membranes from lipid monolayers and study of their electrical properties
26. Detecting protein analytes that modulate transmembrane movement of a polymer chain within a single protein pore
27. Phosphorylation of photolyzed rhodopsin is calcium-insensitive in retina permeabilized by α-toxin
28. Tumor protease-activated, pore-forming toxins from a combinatorial library
29. Reversible permeabilization of plasma membranes with an engineered switchable pore
30. Cyclic peptides as molecular adapters for a pore-forming protein
31. Structure of staphylococcal α-hemolysin, a heptameric transmembrane pore
32. A pore-forming protein with a protease-activated trigger
33. Functional expression of the α-hemolysin of Staphylococcus aureus in intact Escherichia coli and in cell lysates