Opening the KcsA K+ channel: Tryptophan scanning and complementation analysis lead to mutants with altered gating

Biochemistry

Volumn 41, Issue 46, 2002, Pages 13653-13662

  Irizarry, Stacey N ^a   Kutluay, Esin ^a,b   Drews, Gabriele ^a   Hart, Sarah J ^a   Heginbotham, Lise ^a  

^a YALE UNIVERSITY   (United States)

^b Weill Cornell Medical Center   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

TRANSMEMBRANES;

ESCHERICHIA COLI; MEMBRANES; MUTAGENESIS; OLIGOMERS; SCANNING;

BIOCHEMISTRY;

MEMBRANE PROTEIN; OLIGOMER; POTASSIUM CHANNEL; PROTEIN KCSA; TETRAMER; TRYPTOPHAN; UNCLASSIFIED DRUG;

ARTICLE; CHANNEL GATING; CONTROLLED STUDY; ESCHERICHIA COLI; GENETIC COMPLEMENTATION; MUTAGENESIS; NONHUMAN; OLIGOMERIZATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; POTASSIUM TRANSPORT; PRIORITY JOURNAL; PROTEIN STABILITY; WESTERN BLOTTING;

ANIMALS; ANTIBODY FORMATION; BACTERIAL PROTEINS; DIMERIZATION; ELECTRON SPIN RESONANCE SPECTROSCOPY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENETIC COMPLEMENTATION TEST; HYDROGEN-ION CONCENTRATION; ION CHANNEL GATING; IONS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; POTASSIUM; POTASSIUM CHANNELS; PROTEIN BINDING; PROTEIN CONFORMATION; RABBITS; TRYPTOPHAN;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0037137218     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi026393r     Document Type: Article

References (37)

1. Doyle, D. A., Morais Cabral, J., Pfuetzner, R. A., Kuo, A., Gulbis, J. M., Cohen, S. L., Chait, B. T., and MacKinnon, R. (1998) Science 280, 69-77.
2. Zhou, Y., Morais-Cabral, J. H., Kaufman, A., and MacKinnon, R. (2001) Nature 414, 43-48.
3. Morais-Cabral, J. H., Zhou, Y., and MacKinnon, R. (2001) Nature 414, 37-42.
4. Cuello, L. G., Romero, J. G., Cortes, D. M., and Perozo, E. (1998) Biochemistry 37, 3229-3236.
5. Heginbotham, L., LeMasurier, M., Kolmakova-Partensky, L., and Miller, C. (1999) J. Gen. Physiol. 114, 551-560.
6. Liu, Y., Holmgren, M., Jurman, M. E., and Yellen, G. (1997) Neuron 19, 175-184.
7. Heginbotham, L., Odessey, E., and Miller, C. (1997) Biochemistry 36, 10335-10342.
8. Cortes, D. M., and Perozo, E. (1997) Biochemistry 36, 10343-10352.
9. LeMasurier, M., Heginbotham, L., and Miller, C. (2001) J. Gen. Physiol. 118, 303-314.
10. Yellen, G. (1998) Q. Rev. Biophys. 31, 239-295.
11. Perozo, E., Cortes, D. M., and Cuello, L. G. (1999) Science 285, 73-78.
12. Jiang, Y., Lee, A., Chen, J., Cadene, M., Chait, B. T., and MacKinnon, R. (2002) Nature 417, 523-526.
13. Heginbotham, L., Lu, Z., Abramson, T., and MacKinnon, R. (1994) Biophys. J. 66, 1061-1067.
14. Splitt, H., Meuser, D., Borovok, I., Betzler, M., and Schrempf, H. (2000) FEBS Lett. 472, 83-87.
15. Anderson, J. A., Huprikar, S. S., Kochian, L. V., Lucas, W. J., and Gaber, R. F. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 3736-3740.
16. Sentenac, H., Bonneaud, N., Minet, M., Lacroute, F., Salmon, J. M., Gaymard, F., and Grignon, C. (1992) Science 256, 663-665.
17. Nakamura, R. L., Anderson, J. A., and Gaber, R. F. (1997) J. Biol. Chem. 272, 1011-1018.
18. Ou, X., Blount, P., Hoffman, R. J., and Kung, C. (1998) Proc. Natl. Acad. Sci. U.S.A. 95, 11471-11475.
19. Sukharev, S. I., Blount, P., Martinac, B., and Kung, C. (1997) Annu. Rev. Physiol. 59, 633-657.
20. Loukin, S. H., Vaillant, B., Zhou, X. L., Spalding, E. P., Kung, C., and Saimi, Y. (1997) EMBO J. 16, 4817-4825.
21. Minor, D. L., Jr., Masseling, S. J., Jan, Y. N., and Jan, L. Y. (1999) Cell 96, 879-891.
22. Sadja, R., Smadja, K., Alagem, N., and Reuveny, E. (2001) Neuron 29, 669-680.
23. Yi, B. A., Lin, Y. F., Jan, Y. N., and Jan, L. Y. (2001) Neuron 29, 657-667.
24. Epstein, W., Buurman, E., McLaggan, D., and Naprstek, J. (1993) Biochem. Soc. Trans. 21, 1006-1010.
25. Lu, Z., Klem, A. M., and Ramu, Y. (2001) Nature 413, 809-813.
26. Hackos, D. H., Chang, T. H., and Swartz, K. J. (2002) J. Gen. Physiol. 119, 521-532.
27. Matteson, D. R., and Swenson, R. P., Jr. (1986) J. Gen. Physiol. 87, 795-816.
28. Swenson, R. P., Jr., and Armstrong, C. M. (1981) Nature 291, 427-429.
29. Choe, H., Sackin, H., and Palmer, L. G. (1998) J. Gen. Physiol. 112, 433-446.
30. Choe, S., Stevens, C. F., and Sullivan, J. M. (1995) Proc. Natl. Acad. Sci. U.S.A. 92, 12046-12049.
31. Monks, S. A., Needleman, D. J., and Miller, C. (1999) J. Gen. Physiol. 113, 415-423.
32. Hong, K. H., and Miller, C. (2000) J. Gen. Physiol. 115, 51-58.
33. Collins, A., Chuang, H., Jan, Y. N., and Jan, L. Y. (1997) Proc. Natl. Acad. Sci. U.S.A. 94, 5456-5460.
34. Jiang, Y., Lee, A., Chen, J., Cadene, M., Chait, B. T., and MacKinnon, R. (2002) Nature 417, 515-522.
35. Holmgren, M., Shin, K. S., and Yellen, G. (1998) Neuron 21, 617-621.
36. Li-Smerin, Y., and Swartz, K. J. (2001) J. Gen. Physiol. 117, 205-218.
37. Li-Smerin, Y., Hackos, D. H., and Swartz, K. J. (2000) J. Gen. Physiol. 115, 33-50.