Sample sonication after trichloroacetic acid precipitation increases protein recovery from cultured hippocampal neurons, and improves resolution and reproducibility in two-dimensional gel electrophoresis

Electrophoresis

Volumn 27, Issue 9, 2006, Pages 1825-1831

  Manadas, Bruno J ^a   Vougas, Konstantinos ^b   Fountoulakis, Michael ^b   Duarte, Carlos B ^a  

^a UNIVERSITY OF COIMBRA   (Portugal)

^b Academy of Athens Biomedical Research Foundation   (Greece)

Author keywords

2 D SDS PAGE; Hippocampus; Solubilization; Sonication

Indexed keywords

ACETONE; ELECTROPHORESIS; PROTEINS; SONICATION;

2-D SDS-PAGE; ACID PRECIPITATION; HIPPOCAMPAL NEURONS; HIPPOCAMPUS; PROTEIN PRECIPITATION; PROTEIN RECOVERY; REPRODUCIBILITIES; SDS-PAGE; SOLUBILISATION; TWO-DIMENSIONAL GEL ELECTROPHORESIS;

GELS;

BUFFER; TRICHLOROACETIC ACID;

ACID PRECIPITATION; ANIMAL CELL; ARTICLE; COMPUTER ANALYSIS; COMPUTER PROGRAM; CONTROLLED STUDY; ELUTION; HIPPOCAMPUS; NERVE CELL CULTURE; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN ANALYSIS; PROTEIN DEGRADATION; RAT; REPRODUCIBILITY; SAMPLE SIZE; TWO DIMENSIONAL GEL ELECTROPHORESIS; ULTRASOUND;

EID: 33646590654     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/elps.200500757     Document Type: Article

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