PDGF regulates the actin cytoskeleton through hnRNP-K-mediated activation of the ubiquitin E3-ligase MIR

EMBO Journal

Volumn 25, Issue 9, 2006, Pages 1871-1882

  Nagano, Kohji ^a,e   Bornhauser, Beat C ^b,f   Warnasuriya, Gayathri ^a   Entwistle, Alan ^a   Cramer, Rainer ^a,c,g   Lindholm, Dan ^b,d   Naaby Hansen, Soren ^a,c  

^a ROYAL FREE AND UNIVERSITY COLLEGE MEDICAL SCHOOL   (United Kingdom)

^b UPPSALA UNIVERSITY   (Sweden)

^c UNIVERSITY COLLEGE LONDON   (United Kingdom)

^d MINERVA FOUNDATION INSTITUTE FOR MEDICAL RESEARCH   (Finland)

^e UNIVERSITY OF TOKYO   (Japan)

^f UNIVERSITY OF ZURICH   (Switzerland)

^g UNIVERSITY OF READING   (United Kingdom)

Author keywords

Focal adhesions; hnRNP K; MIR; PDGF; Ubiquitin

Indexed keywords

ACTIN; MESSENGER RNA; MITOGENIC AGENT; MYOSIN; MYOSIN LIGHT CHAIN; PLATELET DERIVED GROWTH FACTOR; PROTEASOME; PROTEIN MIR; PROTEIN TYROSINE KINASE; RIBONUCLEOPROTEIN; UBIQUITIN PROTEIN LIGASE E3; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL MIGRATION; CELL MOTILITY; CELL ORGANELLE; CHEMOTAXIS; CONTROLLED STUDY; CYTOSKELETON; ENZYME ACTIVATION; ENZYME INHIBITION; FIBROBLAST; FOCAL ADHESION; GENETIC TRANSCRIPTION; MOUSE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; RNA TRANSLATION; STRESS FIBER; UBIQUITINATION; WOUND HEALING;

ACTINS; ANIMALS; CELL MOVEMENT; CYTOSKELETON; DOWN-REGULATION; ENZYME ACTIVATION; EPIDERMAL GROWTH FACTOR; FIBROBLASTS; HETEROGENEOUS-NUCLEAR RIBONUCLEOPROTEIN K; INSULIN-LIKE GROWTH FACTOR I; MICE; MICROFILAMENTS; MYOSIN LIGHT CHAINS; PLATELET-DERIVED GROWTH FACTOR; PROTEASOME ENDOPEPTIDASE COMPLEX; RNA, SMALL INTERFERING; SRC-FAMILY KINASES; SWISS 3T3 CELLS; UBIQUITIN-PROTEIN LIGASES;

ANIMALIA;

EID: 33646582354     PISSN: 02614189     EISSN: 14602075     Source Type: Journal    
DOI: 10.1038/sj.emboj.7601059     Document Type: Article

References (30)

1. Adams J, Palombella VJ, Sausville EA, Johnson J, Destree A, Lazarus DD, Maas J, Pien CS, Prakash S, Elliott PJ (1999) Proteasome inhibitors: a novel class of potent and effective antitumor agents. Cancer Res 59: 2615-2622
2. Bengur AR, Robinson EA, Apella E, Sellers JR (1987) Sequence of the sites phosphorylated by PKC in the smooth muscle myosin light chain. J Biol Chem 262: 7613-7617
3. Bockus BJ, Stiles CD (1984) Regulation of cytoskeletal architecture by platelet-derived growth factor, insulin and epidermal growth factor. Exp Cell Res 153: 186-197
4. Bomsztyk K, Denisenko O, Ostrowski J (2004) hnRNP K: one protein multiple processes. Bioessays 26: 629-638
5. Bornhauser BC, Olsson P-A, Lindholm D (2003) MSAP is a novel MIR-interacting protein that enhances neurite outgrowth and increases myosin regulatory light chain. J Biol Chem 278: 35412-35420
6. de Hoog CL, Foster LJ, Mann M (2004) RNA and RNA binding proteins participate in early stages of cell spreading through spreading initiation centers. Cell 117: 649-662
7. Entwistle A, Noble M (1994) Optimising the performance of confocal laser scanning microscopes over the full field of view. J Microsc 175: 238-251
8. Fukata Y, Amano M, Kaibuchi K (2001) Rho-Rho-kinase pathway in smooth muscle contraction and cytoskeletal reorganization of non-muscle cells. Trends Pharmacol Sci 22: 32-39
9. Hall A (1998) Rho GTPases and the actin cytoskeleton. Science 279: 509-514
10. Ikebe M, Hartshorne DJ, Elzinga M (1987) Phosphorylation of the 20,000-Dalton light chain of smooth muscle myosin by the calcium-activated, phospholipid-dependent protein kinase. Phosphorylation sites and effects of phosphorylation. J Biol Chem 262: 9569-9573
11. Ikebe M, Koretz J, Hartshorne DJ (1988) Effects of phosphorylation of light chain residues threonine 18 and serine 19 on the properties and conformation of smooth muscle myosin. J Biol Chem 263: 6432-6437
12. Jimenez C, Portela RA, Mellado M, Rodriguez-Frade JM, Collard J, Serrano A, Martinez-A C, Avila J, Carrera AC (2000) Role of the PI3 K regulatory subunit in the control of actin organization and cell migration. J Cell Biol 151: 249-262
13. Kimura K, Ito M, Amano M, Chihara K, Fukata Y, Nakafuku M, Yamamori B, Feng J, Nakano T, Okawa K, Iwamatsu A, Kaibuchi K (1996) Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase). Science 273: 245-248
14. Klimek-Tomczak K, Wyrwicz LS, Jain S, Bomsztyk K, Ostrowski J (2004) Characterization of hnRNP K protein-RNA interactions. J Mol Biol 342: 1131-1141
15. Komatsu S, Ikebe M (2004) ZIP kinase is responsible for the phosphorylation of myosin II and necessary for cell motility in mammalian fibroblasts. J Cell Biol 165: 243-254
16. Kureishi Y, Kobayashi S, Amano M, Kimura K, Kanaide H, Nakano T, Kaibuchi K, Ito M (1997) Rho-associated kinase directly induces smooth muscle contraction through myosin light chain phosphorylation. J Biol Chem 272: 12257-12260
17. Nagano K, Masters JR, Akpan A, Yang A, Corless S, Wood C, Hastie C, Zvelebil M, Cramer R, Naaby-Hansen S (2004) Differential protein synthesis and expression levels in normal and neoplastic human prostate cells and their regulation by type I and II interferons. Oncogene 23: 1693-1703
18. Olsson P-A, Korhonen L, Mercer EA, Lindholm D (1999) MIR is a novel ERM-like protein that interacts with myosin regulatory light chain and inhibits neurite outgrowth. J Biol Chem 274: 36288-36292
19. Ostareck-Lederer A, Ostareck DH, Cans C, Neubauer G, Bomsztyk K, Superti-Furga G, Hentze MW (2002) c-Src-mediated phosphorylation of hnRNP K drives translational activation of specifically silenced mRNAs. Mol Cell Biol 22: 4535-4543
20. Paziewska A, Wyrwicz LS, Bujnicki JM, Bomsztyk K, Ostrowski J (2004) Cooperative binding of the hnRNP K three KH domains to mRNA targets. FEBS Lett 577: 134-140
21. Rankin S, Rozengurt E (1994) Platelet-derived growth factor modulation of focal adhesion kinase (p125FAK) and paxillin tyrosine phosphorylation in Swiss 3T3 cells. Bell-shaped dose response and cross-talk with bombesin. J Biol Chem 269: 704-710
22. Ridley AJ, Hall A (1994) Signal transduction pathways regulating Rho-mediated stress fibre formation: requirement for a tyrosine kinase. EMBO J 13: 2600-2610
23. Satterwhite LL, Lohka MJ, Wilson KL, Scherson TY, Cisek LJ, Corden JL, Pollard TD (1992) Phosphorylation of myosin-II regulatory light chain by cyclin-p34cdc2: a mechanism for the timing of cytokinesis. J Cell Biol 118: 595-605
24. Sellers JR, Pato MD, Adelstein RS (1981) Reversible phosphorylation of smooth muscle myosin, heavy meromyosin, and platelet myosin. J Biol Chem 256: 13137-13142
25. Seufferlein T, Rozengurt E (1994) Lysophosphatidic acid stimulates tyrosine phosphorylation of focal adhesion kinase, paxillin, and p130. Signaling pathways and cross-talk with platelet-derived growth factor. J Biol Chem 269: 9345-9351
26. Thisted T, Lyakhov DL, Liebhaber SA (2001) Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest distinct modes of RNA recognition. J Biol Chem 276: 17484-17496
27. Totsukawa G, Yamakita Y, Yamashiro S, Hartshorne DJ, Sasaki Y, Matsumura F (2000) Distinct roles of ROCK (Rho-kinase) and MRLCK in spatial regulation of MRLC phosphorylation for assembly of stress fibers and focal adhesions in 3T3 fibroblasts. J Cell Biol 150: 797-806
28. Wang HR, Zhang Y, Ozdamar B, Ogunjimi AA, Alexandrova E, Thomsen GH, Wrana JL (2003) Regulation of cell polarity and protrusion formation by targeting RhoA for degradation. Science 302: 1775-1779
29. Wennstrom S, Hawkins P, Cooke F, Hara K, Yonezawa K, Kasuga M, Jackson T, Claesson-Welsh L, Stephens L (1994) Activation of phosphoinositide 3-kinase is required for PDGF-stimulated membrane ruffling. Curr Biol 4: 385-393
30. Wojciak-Stothard B, Entwistle A, Garg R, Ridley AJ (1998) Regulation of TNF-alpha-induced reorganization of the actin cytoskeleton and cell-cell junctions by Rho, Rac, and Cdc42 in human endothelial cells. J Cell Physiol 176: 150-165