Plasmid DNA and siRNA transfection of intestinal epithelial monolayers by electroporation

International Journal of Pharmaceutics

Volumn 315, Issue 1-2, 2006, Pages 122-133

  Ghartey Tagoe, Esi B ^a   Babbin, Brian A ^b   Nusrat, Asma ^b   Neish, Andrew S ^b   Prausnitz, Mark R ^a,c  

^a Emory University   (United States)

^b EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c Georgia Institute of Technology   (United States)

Author keywords

Electroporation; Intestinal epithelium; Lipofection; Plasmid DNA gene transfection; siRNA gene silencing

Indexed keywords

DNA; GREEN FLUORESCENT PROTEIN; LAMIN A; LAMIN C; LUCIFERASE; PLASMID DNA; SMALL INTERFERING RNA;

ARTICLE; CELL DIFFERENTIATION; CONTROLLED STUDY; DNA CONTENT; ELECTROPORATION; GENE EXPRESSION; GENE SILENCING; GENE THERAPY; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; INTESTINE EPITHELIUM; MONOLAYER CULTURE; PRIORITY JOURNAL; PROTEIN EXPRESSION; REPORTER GENE;

CELL LINE; ELECTROPORATION; EPITHELIAL CELLS; GENE SILENCING; GENES, REPORTER; HUMANS; INTESTINAL MUCOSA; PLASMIDS; PROTEINS; RNA, SMALL INTERFERING; TRANSFECTION;

EID: 33646379604     PISSN: 03785173     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ijpharm.2006.02.022     Document Type: Article

References (49)

1. Akaneya Y., Jiang B., and Tsumoto T. RNAi-induced gene silencing by local electroporation in targeting brain region. J. Neurophysiol. 93 (2005) 594-602
2. Artursson P., Palm K., and Luthman K. Caco-2 monolayers in experimental and theoretical predictions of drug transport. Adv. Drug Deliv. Rev. 46 (2001) 27-43
3. Balamurugan K., Ortiz A., and Said H.M. Biotin uptake by human intestinal and liver epithelial cells: role of the SMVT system. Am. J. Physiol. Gastrointest. Liver Physiol. 285 (2003) G73-G77
4. Bergan R., Connell Y., Fahmy B., and Neckers L. Electroporation enhances c-myc antisense oligodeoxynucleotide efficacy. Nucl. Acids Res. 21 (1993) 3567-3573
5. Bichko V.V. Cationic liposomes. In: Ravid K., and Freshney R.I. (Eds). DNA Transfer to Cultured Cells (1998), Wiley/Liss, New York 193-211
6. Buckles D.C., Forster J., and McCallum R.W. The treatment of gastroparesis in the age of the gastric pacemaker: a review. Med. Gen. Med. 5 (2003) 5
7. Bureau M.F., Gehl J., Deleuze V., Mir L.M., and Scherman D. Importance of association between permeabilization and electrophoretic forces for intramuscular DNA electrotransfer. Biochim. Biophys. Acta 1474 (2000) 353-359
8. Canatella P.J., Karr J.F., Petros J.A., and Prausnitz M.R. Quantitative study of electroporation-mediated molecular uptake and cell viability. Biophys. J. 80 (2001) 755-764
9. Canatella P.J., and Prausnitz M.R. Prediction and optimization of gene transfection and drug delivery by electroporation. Gene Ther. 8 (2001) 1464-1469
10. In: Chang D.C., Chassy B.M., Saunders J.A., and Sowers A.E. (Eds). Guide to Electroporation and Electrofusion (1992), Academic Press, New York
11. Check E. Gene regulation: RNA to the rescue?. Nature 425 (2003) 10-12
12. Crystal R.G. Transfer of genes to humans: early lessons and obstacles to success. Science 270 (1995) 404-410
13. Dharmsathaphorn K., and Madara J.L. Established intestinal cell lines as model systems for electrolyte transport studies. Meth. Enzymol. 192 (1990) 354-389
14. Dunne J., Drescher B., Riehle H., Hadwiger P., Young B.D., Krauter J., and Heidenreich O. The apparent uptake of fluorescently labeled siRNAs by electroporated cells depends on the fluorochrome. Oligonucleotides 13 (2003) 375-380
15. Elbashir S.M., Harborth J., Lendeckel W., Yalcin A., Weber K., and Tuschl T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411 (2001) 494-498
16. Elbashir S.M., Lendeckel W., and Tuschl T. RNA interference is mediated by 21- and 22-nucleotide RNAs. Genes Dev. 15 (2001) 188-200
17. Elbashir S.M., Harborth J., Weber K., and Tuschl T. Analysis of gene function in somatic mammalian cells using small interfering RNAs. Methods 26 (2002) 199-213
18. Felgner P.L., Gadek T.R., Holm M., Roman R., Chan H.W., Wenz M., Northrop J.P., Ringold G.M., and Danielsen M. Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure. Proc. Natl. Acad. Sci. U.S.A. 84 (1987) 7413-7417
19. Ghartey-Tagoe, E.B., 2004. Electroporation-mediated delivery of macromolecules to intestinal epithelial models. Ph.D. Thesis. Georgia Institute of Technology, Atlanta, GA.
20. Ghartey-Tagoe E.B., Morgan J.S., Ahmed K., Neish A.S., and Prausnitz M.R. Electroporation-mediated delivery of molecules to model intestinal epithelia. Int. J. Pharm. 270 (2004) 127-138
21. Gothelf A., Mir L.M., and Gehl J. Electrochemotherapy: results of cancer treatment using enhanced delivery of bleomycin by electroporation. Cancer Treat Rev. 29 (2003) 371-387
22. Hannon G.J. RNA interference. Nature 418 (2002) 244-251
23. Harborth J., Elbashir S.M., Bechert K., Tuschl T., and Weber K. Identification of essential genes in cultured mammalian cells using small interfering RNAs. J. Cell Sci. 114 (2001) 4557-4565
24. Jiang Z.Y., Zhou Q.L., Coleman K.A., Chouinard M., Boese Q., and Czech M.P. Insulin signaling through Akt/protein kinase B analyzed by small interfering RNA-mediated gene silencing. Proc. Natl. Acad. Sci. U.S.A. 100 (2003) 7569-7574
25. Kishida T., Asada H., Gojo S., Ohashi S., Shin-Ya M., Yasutomi K., Terauchi R., Takahashi K.A., Kubo T., Imanishi J., and Mazda O. Sequence-specific gene silencing in murine muscle induced by electroporation-mediated transfer of short interfering RNA. J. Gene Med. 6 (2004) 105-110
26. Klenchin V.A., Sukharev S.I., Serov S.M., Chernomordik L.V., and Chizmadzhev Yu A. Electrically induced DNA uptake by cells is a fast process involving DNA electrophoresis. Biophys. J. 60 (1991) 804-811
27. Lechardeur D., Sohn K.J., Haardt M., Joshi P.B., Monck M., Graham R.W., Beatty B., Squire J., O'Brodovich H., and Lukacs G.L. Metabolic instability of plasmid DNA in the cytosol: a potential barrier to gene transfer. Gene Ther. 6 (1999) 482-497
28. Li L.H., Sen A., Murphy S.P., Jahreis G.P., Fuji H., and Hui S.W. Apoptosis induced by DNA uptake limits transfection efficiency. Exp. Cell Res. 253 (1999) 541-550
29. Matsui H., Johnson L.G., Randell S.H., and Boucher R.C. Loss of binding and entry of liposome-DNA complexes decreases transfection efficiency in differentiated airway epithelial cells. J. Biol. Chem. 272 (1997) 1117-1126
30. Muller K.J., Horbaschek M., Lucas K., Zimmermann U., and Sukhorukov V.L. Electrotransfection of anchorage-dependent mammalian cells. Exp. Cell Res. 288 (2003) 344-353
31. Neumann E., Schaefer-Ridder M., Wang Y., and Hofschneider P.H. Gene transfer into mouse lyoma cells by electroporation in high electric fields. EMBO J. 1 (1982) 841-845
32. Neumann E., Sowers A.E., and Jordan C.A. Electroporation and Electrofusion in Cell Biology (1989), Plenum Press, New York
33. Oliveira D.M., and Goodell M.A. Transient RNA interference in hematopoietic progenitors with functional consequences. Genesis 36 (2003) 203-208
34. Ovcharenko D., Jarvis R., Hunicke-Smith S., Kelnar K., and Brown D. High-throughput RNAi screening in vitro: from cell lines to primary cells. RNA 11 (2005) 985-993
35. Pouton C.W. Nuclear import of polypeptides, polynucleotides and supramolecular complexes. Adv. Drug Deliv. Rev. 34 (1998) 51-64
36. Ravid K., and Freshney R.I. DNA Transfer to Cultured Cells (1998), Wiley/Liss, New York
37. Satkauskas S., Bureau M.F., Puc M., Mahfoudi A., Scherman D., Miklavcic D., and Mir L.M. Mechanisms of in vivo DNA electrotransfer: respective contributions of cell electropermeabilization and DNA electrophoresis. Mol. Ther. 5 (2002) 133-140
38. Shi Y. Mammalian RNAi for the masses. Trends Genet. 19 (2003) 9-12
39. Shimokawa T., Okumura K., and Ra C. DNA induces apoptosis in electroporated human promonocytic cell line U937. Biochem. Biophys. Res. Commun. 270 (2000) 94-99
40. Shuey D.J., McCallus D.E., and Giordano T. RNAi: gene-silencing in therapeutic intervention. Drug Discov. Today 7 (2002) 1040-1046
41. Sukharev S.I., Klenchin V.A., Serov S.M., Chernomordik L.V., and Chizmadzhev Yu A. Electroporation and electrophoretic DNA transfer into cells. The effect of DNA interaction with electropores. Biophys. J. 63 (1992) 1320-1327
42. Trezise A.E. In vivo DNA electrotransfer. DNA Cell Biol. 21 (2002) 869-877
43. Tucker T.A., Varga K., Bebok Z., Zsembery A., McCarty N.A., Collawn J.F., Schwiebert E.M., and Schwiebert L.M. Transient transfection of polarized epithelial monolayers with CFTR and reporter genes using efficacious lipids. Am. J. Physiol. Cell Physiol. 284 (2003) C791-C804
44. Uduehi A.N., Moss S.H., Nuttall J., and Pouton C.W. Cationic lipid-mediated transfection of differentiated Caco-2 cells: a filter culture model of gene delivery to a polarized epithelium. Pharm. Res. 16 (1999) 1805-1811
45. Wilson J.A., Jayasena S., Khvorova A., Sabatinos S., Rodrigue-Gervais I.G., Arya S., Sarangi F., Harris-Brandts M., Beaulieu S., and Richardson C.D. RNA interference blocks gene expression and RNA synthesis from hepatitis C replicons propagated in human liver cells. Proc. Natl. Acad. Sci. U.S.A. 100 (2003) 2783-2788
46. Xie T.D., Sun L., and Tsong T.Y. Study of mechanisms of electric field-induced DNA transfection. I. DNA entry by surface binding and diffusion through membrane pores. Biophys. J. 58 (1990) 13-19
47. Yang T.A., Heiser W.C., and Sedivy J.M. Efficient in situ electroporation of mammalian cells grown on microporous membranes. Nucl. Acids Res. 23 (1995) 2803-2810
48. Yu J.Y., DeRuiter S.L., and Turner D.L. RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells. Proc. Natl. Acad. Sci. U.S.A. 99 (2002) 6047-6052
49. Zamore P.D. Ancient pathways programmed by small RNAs. Science 296 (2002) 1265-1269