Metabolic instability of plasmid DNA in the cytosol: A potential barrier to gene transfer

Gene Therapy

Volumn 6, Issue 4, 1999, Pages 482-497

  Lechardeur, D ^a   Sohn, K J ^a   Haardt, M ^a   Joshi, P B ^b   Monck, M ^b   Graham, R W ^b   Beatty, B ^c   Squire, J ^c   O'Brodovich, H ^d   Lukacs, G L ^a,c  

^a HOSPITAL FOR SICK CHILDREN   (Canada)

^b Inex Pharmaceuticals   (Canada)

^c UNIVERSITY OF TORONTO   (Canada)

^d UNIVERSITY OF TORONTO   (Canada)

Author keywords

Degradation; DNase; Gene transfer; Microinjection; Plasmid DNA; Turnover

Indexed keywords

CELL ENZYME; DEOXYRIBONUCLEASE; DNA NUCLEOTIDYLEXOTRANSFERASE; DOUBLE STRANDED DNA; NUCLEASE; PHOSPHOLIPID; PLASMID DNA; SINGLE STRANDED DNA;

APOPTOSIS; ARTICLE; CELL DIVISION; CELL STRAIN COS1; CONTROLLED STUDY; CYTOSOL; DNA DEGRADATION; ENCAPSULATION; ENDOCYTOSIS; ENZYME ACTIVITY; FLUORESCENCE IN SITU HYBRIDIZATION; GENE TARGETING; GENE TRANSFER; HELA CELL; HUMAN; HUMAN CELL; INTERNALIZATION; MICROINJECTION; PRIORITY JOURNAL; SOUTHERN BLOTTING;

BLOTTING, SOUTHERN; CALCIUM; CYTOSOL; DEOXYRIBONUCLEASES; DIGITONIN; GENE TRANSFER TECHNIQUES; HELA CELLS; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; MICROINJECTIONS; MICROSCOPY, FLUORESCENCE; PLASMIDS; TRANSFECTION;

EID: 0032962975     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3300867     Document Type: Article

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