Optimization of the citric acid production by Aspergillus niger through a metabolic flux balance model

Electronic Journal of Biotechnology

Volumn 4, Issue 1, 2001, Pages 1-17

  Guebel, Daniel V ^a   Torres Darias, Néstor V ^b  

^a Biotechnology Counseling Services   (Argentina)

^b UNIVERSIDAD DE LA LAGUNA   (Spain)

Author keywords

Bioenergetics; Metabolic engineering; Metabolic pathway; Stoichiometry

Indexed keywords

EID: 33646227276     PISSN: 07173458     EISSN: 07173458     Source Type: Journal    
DOI: None     Document Type: Article

References (51)

1. Alberts, B.; Bray, D.; Lewis, J.; Raff, M.; Roberts, K. and Watson, J.D. (1994). Molecular biology of the cell. Garland Publishing, Inc. New York. 805 p.
2. Alvarez-Vazquez, F.; González-Alcón, C. and Torres, N.V. (2000). Metabolism of citric acid production by Aspergillus niger: model definition, steady state analysis and constrained optimization of the citric acid production rate. Biotechnology and Bioengineering 70:82-108.
3. Andrews, G.F. (1993). The yield in the modeling and control of bioprocesses. Biotechnology and Bioengineering 42:549-556.
4. Bonarius, H.P.J.; Hatzimanikatis, V.; Meesters, K.P.; de Gooijer, C.D.; Schmidt, G. and Tramper, J. (1995). Metabolic flux analysis of hybridoma cells in different culture media using mass balances. Biotechnology and Bioengineering 50:299-318.
5. Delgado, J. and Liao, J.C. (1997). Inverse flux analysis for reduction of acetate excretion in Escherichia coli. Biotechnology Progress 13:361-367.
6. Erickson, L.E.; Minkevich, I.G. and Eroshin, V.K. (1978). Application of mass and energy regularities in fermentation. Biotechnology and Bioengineering XX:1595-1621.
7. Habison, A.C.; Kubicek, C.P. and Röhr, M. (1979). Phosphofructokinase as a regulatory enzyme in citric acid producing A. niger. FEMS Microbiology Letters 5:39-42.
8. 31P NMR in Aspergillus niger. Journal of Biotechnology 77:5-15.
9. Jernejc, K. and Cimerman, A. (1992). Composition of Aspergillus niger mycelium during growth on productive and unproductive substrates. Journal of Biotechnology 25:341-348.
10. Jorgensen, H.; Nielsen, J.; Villadsen, J. and Mollgaard, H. (1995). Metabolic flux distribution in Penicillium chrysogenum during fed batch cultivation. Biotechnology and Bioengineering 46:117-131.
11. Kirimura, K.; Hirowatari, Y. and Usami, S. (1987). Alterations of respiratory systems in Aspergillus niger under the conditions of citric acid fermentation. Agriculture Biological Chemistry 51:1299-1303.
12. Kisser, M.; Kubicek, C.P. and Röhr, M. (1980). Influence of manganese in morphology and cell wall composition of Aspergillus niger during citric acid fermentation. Archives of Microbiology 128:26-33.
13. 2 fixation by Klebsiella pneumoniae. Federation European Biological Societies 187:237-239.
14. Kristiansen, B.; Mattey, M. and Linden, J. (1998). Citric acid biotechnology. Kristiansen, B.; Mattey, M. and Linden, J. eds. Taylor and Francis, London. UK. 250 p.
15. Kristiansen, B. and Sinclair, G. (1978). Production of citric acid in batch culture. Biotechnology and Bioengineering 20:1711-1722.
16. Kubicek, C.P.; Witteveen, C.F.B. and Visser, J. (1994). Regulation of organic acid production by Aspergilli. In: Powell, K.A. ed. The Genus Aspergillus. Plenum Press. New York. pp. 35-145.
17. Kubicek, C.P. and Röhr, M. (1986). Citric acid fermentation. CRC Critical Reviews in Biotechnology 3:331-373.
18. Kubicek, C.P.; Hampel, W. and Röhr, M. (1979). Manganese deficiency leads to elevated amino acid pools in citric acid accumulating Aspergillus niger. Archives of Microbiology 123:73-79.
19. Kumar, S.; Punekar, N.S.; SatyaNarayan, V. and Venkatesh, K.V. (2000). Metabolic fate of glutamate and evaluation of flux trough the 4-aminobutyrate (GABA) shunt in Aspergillus niger. Biotechnology and Bioengineering 67:575-584.
20. Kumar, S. and Punekar, N.S. (1997). The metabolism of 4-aminobutirate (GABA) in fungi. Mycology Research 101:403-409.
21. Legisa, M. and Mattey, M. (1988). Glycerol synthesis by Aspergillus niger under citric acid accumulating conditions. Enzyme Microbiology and Technology 8:607-609.
22. Ma, H.; Kubicek, C.P. and Röhr, M. (1985). Metabolic effects of manganese deficiency in Aspergillus niger: evidence for increased protein degradation. Archives of Microbiology 141:266-268.
23. Netik, A.; Torres, N.V.; Riol, J.M. and Kubicek, C.P. (1997). Uptake and export of citric acid by Aspergillus niger is reciprocally regulated by manganese ions. Biochimica et Biophysica Acta 1326:287-294.
24. 31P NMR studies on the role of the vacuole in Phosphate metabolism in yeast. Archives of Microbiology 134:270-275.
25. Niranjan, S.C. and San, K.Y. (1988). Analysis of a framework using material balances in metabolic pathways to elucidate cellular metabolism. Biotechnology and Bioengineering 34:496-501.
26. Noorman, H.J.; Heijnen, J.J. and Luyben, K.C.M. (1991). Linear relation in microbial reaction systems: a general overview of their origin, form and use. Biotechnology and Bioengineering 38:603-618.
27. Omar, S.H.; Honecker, S. and Rehm, H.J. (1992). A comparative study on the formation of citric acid and polyols and on morphological changes of three strains of free and immobilized Aspergillus niger. Applied Microbiology and Biotechnology 36:518-524.
28. Pirt, S. J. (1975). Energy and carbon source requirements. In: Principles of microbe and cell cultivation. Blackwell Scientific Publications, Oxford, UK. pp. 63-81.
29. Prömper, C.; Schneider, R. and Weiss, H. (1993). The role of the proton-pumping and alternative respiratory chain NADH: ubiquinone oxidoreductases in overflow catabolism of Aspergillus niger. European Journal of Biochemistry 216:223-230.
30. Roels, J.A. (1983). Energetic and kinetics in biotechnology. Elsevier. Amsterdam. 330 p.
31. Röhr, M. (1998). A century of citric acid fermentation and research. Food Technology and Biotechnology 36:163-171.
32. Röhr, M.; Kubicek, C.P. and Kominek, J. (1996). Citric acid. In: Röhr, M. ed. Biotechnology (Vol. 6). VCH. Wenheim. New York. pp. 307-366.
33. Röhr, M.; Kubicek, C.P.; Zehentgruber, O. and Orthofer, R. (1987). Accumulation and partial reconsumption of polyols and partial reconsumption of polyols during citric acid fermentation by Aspergillus niger. Applied Microbiology and Biotechnology 27:235-239.
34. Ruijter, G.J.; Panneman, H., Xu; D. and Visser, J. (2000). Properties of Aspergillus niger citrate synthase and effects of citA overexpression on citric acid production. Federation European Microbiology Societies Microbiology Letters 184:35-40.
35. Ruijter, G.J.; Panneman, H. and Visser, J. (1997). Overexpression of phosphofructokinase and pyruvate kinase in a citric acid-producing Aspergillus niger. Biochimica et Biophysica Acta 1334:317-326.
36. 13C tracer experiments and metabolite balancing for metabolic flux analysis: comparing two approaches. Biotechnology and Bioengineering 58:254-257.
37. Schmidt, M.; Wallrath, J.; Dorner, A. and Weiss, H. (1992). Disturbed assembly of the respiratory chain NADH: ubiquinone reductase (complex I) in citric acid accumulating Aspergillus niger strain B60. Applied Microbiology and Biotechnology 36:667-672.
38. Torres, N.V.; Voit, E.O.; González-Alcón, C. and Rodríguez, F. (1998). A novel approach for design an overexpression strategy for metabolic engineering. Application to the carbohydrate metabolism in the citric acid producing mould Aspergillus niger. Food Technology and Biotechnology 36:177-1184.
39. Torres, N.V.; Riol-Cimas, J.M.; Wollschek, M. and Kubicek, C.P. (1996). Glucose transport by Aspergillus niger: the low-affinity carrier is only formed during growth on high glucose concentrations. Applied Microbiology and Biotechnology 44:790-794.
40. Torres, N.V.; Voit, E.O. and González-Alcón, C. (1995). Optimization of nonlinear biotechnological process with linear programming: application to citric acid production by Aspergillus niger. Biotechnology and Bioengineering 49:247-258.
41. Torres, N.V. (1994 a). Modeling approach to control of carbohydrate metabolism during citric acid accumulation by Aspergillus niger: I. Model definition and stability of the steady state. Biotechnology and Bioengineering 44:104-111.
42. Torres, N.V. (1994 b). Modeling a pproach to control of carbohydrate metabolism during citric acid accumulation by Aspergillus niger: II. Sensitivity Analysis. Biotechnology and Bioengineering 44:112-118.
43. Vallino, J.J. and Stephanopoulos, G. (1993). Metabolic flux distributions in Corynebacterium glutamicum during growth and lysine overproduction. Biotechnology and Bioengineering 41:633-646.
44. Vanrollegehem, P.A.; de Jong-Gubbels, P.; van Gulik, W.M.; Pronk, J.T.; van Dijken, J.P. and Heijnen, S. (1996). Validation of a Metabolic Network for Saccharomyces cerevisiae using mixed substrates studies. Biotechnology Progress 12:434-448.
45. Verdoes, J.C.; Punt, P.J.; Van der Berg, P.; Debets, F.; Stouthamer, A.H. and Van den Hondel, C.A. (1994). Characterization of an efficient gene cloning strategy for Aspergillus niger based on an autonomously replicating plasmid: cloning of the nicB gene of Aspergillus niger. Gene 146:159-65.
46. Verhoff, F.H. and Spradlin, J.E. (1976). Mass and energy balance analysis of metabolic pathways applied to citric acid production by Aspergillus niger. Biotechnology and Bioengineering XVIII:425-432.
47. Wallrath, J.; Schmidt, M. and Weiss, H. (1991). Concomitant loss of respiratory chain NADH: ubiquinone reductase (complex I) and citric acid accumulation in Aspergillus niger. Applied Microbiology and Biotechnology 36:76-81.
48. Warnacke, J. and Slayman, C. (1980). Metabolic modulation of stoichiometry in a proton pump. Biochimica et Biophysica Acta 591:224-233.
49. Wayman, F.M. and Mattey, M. (2000). Simple diffusion is the primary mechanism for glucose uptake during the production phase of the Aspergillus niger citric acid process. Biotechnology and Bioengineering 67:451-456.
50. Wu, P.; Ray, N.G. and Shuler, M.L. (1993). A computer model for intracellular pH regulation in Chinese hamster ovary cells. Biotechnology Progress 9:374-384.
51. Yu, J. Chang; P. Bhatnagar, D. and Cleveland, T.E. (2000). Cloning of a sugar utilization gene cluster in Aspergillus parasiticus. Biochimica et Biophysica Acta 1493:211-214