Identification of an N-terminal glycogen synthase kinase 3 phosphorylation site which regulates the functional localization of polycystin-2 in vivo and in vitro

Human Molecular Genetics

Volumn 15, Issue 9, 2006, Pages 1465-1473

  Streets, Andrew J ^a   Moon, David J ^a   Kane, Michelle E ^b   Obara, Tomoko ^b   Ong, Albert C M ^a  

^a UNIVERSITY OF SHEFFIELD   (United Kingdom)

^b METROHEALTH MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GLYCOGEN SYNTHASE KINASE 3; GLYCOGEN SYNTHASE KINASE 3 INHIBITOR; MESSENGER RNA; OLIGONUCLEOTIDE; POLYCYSTIN 2; SERINE;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL COMPARTMENTALIZATION; CELL MEMBRANE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; GENETIC CONSERVATION; GLOMERULUS; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; IN VITRO STUDY; IN VIVO STUDY; KIDNEY POLYCYSTIC DISEASE; KIDNEY TUBULE; MORPHOLOGY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; REGULATORY MECHANISM; SITE DIRECTED MUTAGENESIS; WILD TYPE; ZEBRA FISH;

ANIMALS; CELL LINE; DOGS; GLYCOGEN SYNTHASE KINASE 3; HUMANS; PEPTIDE FRAGMENTS; PHOSPHORYLATION; POLYCYSTIC KIDNEY, AUTOSOMAL DOMINANT; PROTEIN STRUCTURE, TERTIARY; TRPP CATION CHANNELS; ZEBRAFISH;

ANIMALIA; DANIO RERIO; VERTEBRATA;

EID: 33646155954     PISSN: 09646906     EISSN: 14602083     Source Type: Journal    
DOI: 10.1093/hmg/ddl070     Document Type: Article

References (31)

1. Harris, P.C. (2002) Molecular basis of polycystic kidney disease: PKD1, PKD2 and PKHD1. Curr. Opin. Nephrol. Hypertens., 11, 309-314.
2. Calvet, J.P. and Grantham, J.J. (2001) The genetics and physiology of polycystic kidney disease. Semin. Nephrol., 21, 107-123.
3. Ong, A.C. and Harris, P.C. (2005) Molecular pathogenesis of ADPKD: The polycystin complex gets complex. Kidney Int., 67, 1234-1247.
4. Streets, A.J., Newby, L.J., O'Hare, M.J., Bukanov, N.O., Ibraghimov-Beskrovnaya, O. and Ong, A.C. (2003) Functional analysis of PKD1 transgenic lines reveals a direct role for polycystin-1 in mediating cell-cell adhesion. J. Am. Soc. Nephrol., 14, 1804-1815.
5. Qian, F., Wei, W., Germino, G. and Oberhauser, A. (2005) The nanomechanics of polycystin-1 extracellular region. J. Biol. Chem., 280, 40723-40730.
6. Montell, C., Birnbaumer, L., Flockerzi, V., Bindels, R.J., Bruford, E.A., Caterina, M.J., Clapham, D.E., Harteneck, C., Heller, S., Julius, D. et al. (2002) A unified nomenclature for the superfamily of TRP cation channels. Mol. Cell, 9, 229-231.
7. Tsiokas, L., Arnould, T., Zhu, C., Kim, E., Walz, G. and Sukhatme, V.P. (1999) Specific association of the gene product of PKD2 with the TRPC1 channel. Proc. Natl. Acad. Sci. USA, 96, 3934-3939.
8. Newby, L.J., Streets, A.J., Zhao, Y., Harris, P.C., Ward, C.J. and Ong, A.C. (2002) Identification, characterization, and localization of a novel kidney polycystin-1-polycystin-2 complex. J. Biol. Chem., 277, 20763-20773.
9. McGrath, J., Somlo, S., Makova, S., Tian, X. and Brueckner, M. (2003) Two populations of node monocilia initiate left-right asymmetry in the mouse. Cell, 114, 61-73.
10. 2+-permeable nonselective cation channel. Proc. Natl. Acad. Sci. USA, 98, 1182-1187.
11. Nauli, S.M., Alenghat, F.J., Luo, Y., Williams, E., Vassilev, P., Li, X., Elia, A.E., Lu, W., Brown, E.M., Quinn, S.J. et al. (2003) Polycystins 1 and 2 mediate mechanosensation in the primary cilium of kidney cells. Nat. Genet., 33, 129-137.
12. Luo, Y., Vassilev, P.M., Li, X., Kawanabe, Y. and Zhou, J. (2003) Native polycystin 2 functions as a plasma membrane ca(2+)-permeable cation channel in renal epithelia. Mol. Cell. Biol., 23, 2600-2607.
13. Ma, R., Li, W.P., Rundle, D., Kong, J., Akbarali, H.I. and Tsiokas, L. (2005) PKD2 functions as an epidermal growth factor-activated plasma membrane channel. Mol. Cell. Biol., 25, 8285-8298.
14. Koulen, P., Cai, Y., Geng, L., Maeda, Y., Nishimura, S., Witzgall, R., Ehrlich, B.E. and Somlo, S. (2002) Polycystin-2 is an intracellular calcium release channel. Nat. Cell. Biol., 4, 191-197.
15. Cohen, P. (2000) The regulation of protein function by multisite phosphorylation-a 25 year update. Trends Biochem. Sci., 25, 596-601.
16. Cai, Y., Anyatonwu, G., Okuhara, D., Lee, K.B., Yu, Z., Onoe, T., Mei, C.L., Qian, Q., Geng, L., Wiztgall, R. et al. (2004) Calcium dependence of polycystin-2 channel activity is modulated by phosphorylation at Ser812. J. Biol. Chem., 279, 19987-19995.
17. Kottgen, M., Benzing, T., Simmen, T., Tauber, R., Buchholz, B., Feliciangeli, S., Huber, T.B., Schermer, B., Kramer-Zucker, A., Hopker, K. et al. (2005) Trafficking of TRPP2 by PACS proteins represents a novel mechanism of ion channel regulation. EMBO J., 24, 705-716.
18. Mochizuki, T., Wu, G., Hayashi, T., Xenophontos, S.L., Veldhuisen, B., Saris, J.J., Reynolds, D.M., Cai, Y., Gabow, P.A., Pierides, A. et al. (1996) PKD2, a gene for polycystic kidney disease that encodes an integral membrane protein. Science, 272, 1339-1342.
19. Li, Q., Montalbetti, N., Shen, P.Y., Dai, X.Q., Cheeseman, C.I., Karpinski, E., Wu, G., Cantiello, H.F. and Chen, X.Z. (2005) Alpha-actinin associates with polycystin-2 and regulates its channel activity. Hum. Mol. Genet., 14, 1587-1603.
20. Sun, Z., Amsterdam, A., Pazour, G.J., Cole, D.G., Miller, M.S. and Hopkins, N. (2004) A genetic screen in zebrafish identifies cilia genes as a principal cause of cystic kidney. Development, 131, 4085-4093.
21. Scheffers, M.S., Le, H., van der Bent, P., Leonhard, W., Prins, F., Spruit, L., Breuning, M.H., de Heer, E. and Peters, D.J. (2002) Distinct subcellular expression of endogenous polycystin-2 in the plasma membrane and Golgi apparatus of MDCK cells. Hum. Mol. Genet., 11, 59-67.
22. Vassilev, P.M., Guo, L., Chen, X.Z., Segal, Y., Peng, J.B., Basora, N., Babakhanlou, H., Cruger, G., Kanazirska, M., Ye, C. et al. (2001) Polycystin-2 is a novel cation channel implicated in defective intracellular Ca(2+) homeostasis in polycystic kidney disease. Biochem. Biophys. Res. Commun., 282, 341-350.
23. Valentijn, J.A., Fyfe, G.K. and Canessa, C.M. (1998) Biosynthesis and processing of epithelial sodium channels in Xenopus oocytes. J. Biol. Chem., 273, 30344-30351.
24. Bezzerides, V.J., Ramsey, I.S., Kotecha, S., Greka, A. and Clapham, D.E. (2004) Rapid vesicular translocation and insertion of TRP channels. Nat. Cell. Biol., 6, 709-720.
25. Montell, C. (2004) Exciting trips for TRPs. Nat. Cell. Biol., 6, 690-692.
26. Ong, A.C., Ward, C.J., Butler, R.J., Biddolph, S., Bowker, C., Torra, R., Pei, Y. and Harris, P.C. (1999) Coordinate expression of the autosomal dominant polycystic kidney disease proteins, polycystin-2 and polycystin-1, in normal and cystic tissue. Am. J. Pathol., 154, 1721-1729.
27. Li, Q., Dai, Y., Guo, L., Liu, Y., Hao, C., Wu, G., Basora, N., Michalak, M. and Chen, X.Z. (2003) Polycystin-2 associates with tropomyosin-1, an actin microfilament component. J. Mol. Biol., 325, 949-962.
28. Westerfield, M. (1995) The zebrafish book. A guide for the laboratory use of zebrafish (Brachydanio). University of Oregon Press, Eugene, OR.
29. Kimmel, C.B., Ballard, W.W., Kimmel, S.R., Ullmann, B. and Schilling, T.F. (1995) Stages of embryonic development of the zebrafish. Dev. Dyn., 203, 253-310.
30. Simons, M., Gloy, J., Ganner, A., Bullerkotte, A., Bashkurov, M., Kronig, C., Schermer, B., Benzing, T., Cabello, O.A., Jenny, A. et al. (2005) Inversin, the gene product mutated in nephronophthisis type II, functions as a molecular switch between Wnt signaling pathways. Nat. Genet., 37, 537-543.
31. Drummond, I.A., Majumdar, A., Hentschel, H., Elger, M., Solnica-Krezel, L., Schier, A.F., Neuhauss, S.C., Stemple, D.L., Zwartkruis, F., Rangini, Z. et al. (1998) Early development of the zebrafish pronephros and analysis of mutations affecting pronephric function. Development, 125, 4655-4667.