Stabilization of recombinant Drosophila acetylcholinesterase

Protein Expression and Purification

Volumn 12, Issue 2, 1998, Pages 166-172

  Estrada Mondaca, Sandino ^a   Fournier, Didier ^a  

^a UNIVERSITÉ DE TOULOUSE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYLCHOLINESTERASE; AFFINITY CHROMATOGRAPHY; DROSOPHILA MELANOGASTER; ENZYME STABILITY; ION EXCHANGE CHROMATOGRAPHY; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; SDS POLYACRYLAMIDE GEL ELECTROPHORESIS;

BOVINAE; DROSOPHILA MELANOGASTER; MELANOGASTER;

EID: 0032030173     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1997.0831     Document Type: Article

References (25)

1. Melanson S. W., Yun C.-H., Pezzementi M., Pezzementi L. Characterization of acetylcholinesterase activity from Drosophila melanogaster. Comp. Biochem. Physiol. 81C:1985;87-96.
2. Gnagey A. L., Forte M., Rosenberry T. L. Isolation and characterization of acetylcholinesterase from Drosophila. J. Biol. Chem. 262:1987;13290-13298.
3. Fournier D., Cuany A., Bride J. M., Bergé J. B. Molecular polymorphism of head acetylcholinesterase from adult houseflies (Musca domestica. J. Neurochem. 49:1987;1455-1461.
4. Toutant J. P., Arpagaus M., Fournier D. Native molecular forms of head acetylcholinesterase from adultDrosophila melanogaster. J. Neurochem. 50:1988;209-218.
5. Mutero A., Fournier D. Post-translational modifications ofDrosophilaIn vitroXenopus. J. Biol. Chem. 267:1992;1695-1700.
6. Fournier D., Bride J. M., Karch F., Berge J. B. Acetylcholinesterase fromDrosophila melanogaster. FEBS Lett. 238:1988a;333-337.
7. Haas R., Marshall T. L., Rosenberry T. L. Drosophila. Biochemistry. 27:1988;6453-6457.
8. Fournier D., Bergé J. B., Cardoso de Almeida M. L., Bordier C. Acetylcholinesterases fromMusca domesticaDrosophila melanogaster. J. Neurochem. 50:1988b;1158-1163.
9. Belzunces L. P., Toutant J.-P., Bounias M. Acetylcholinesterase fromApis mellifera. Biochem. J. 255:1988;463-470.
10. Chaabihi H., Fournier D., Fedon Y., Bossy J. P., Ravallec M., Devauchelle G., Cerruti M. Biochemical characterization ofDrosophila melanogaster. Biochem. Biophys. Res. Commun. 203:1994;734-742.
11. Steele R. W., Smallman B. N. Acetylcholinesterase of the house-fly head affinity purification and subunit composition. Biochem. Biophys. Acta. 445:1976;147-157.
12. Xu G., Bull D. L. Acetylcholinesterase from the horn fly (Diptera: Muscidae): Distribution and purification. J. Econ. Entomol. 87:1994;20-26.
13. Michaelson S., Small D. H. A protease is recovered with a dimeric form of acetylcholinesterase in fetal bovine serum. Brain Res. 611:1993;75-80.
14. Small D. H., Moir R. D., Fuller S. J., Michaelson S., Bush A. I., Li Q. X., Milward E., Hilbich C., Weidemann A., Beyreuther K., Masters C. L. A protease activity associated with acetylcholinesterase releases the membrane-bound form of the amyloid protein precursor of Alzheimer's disease. Biochemistry. 30:1991;10795-10799.
15. Checler F., Vincent J. P. Peptidasic activities associated with acetylcholinesterase are due to contaminating enzymes. J. Neurochem. 53:1989;924-928.
16. Checler F., Grassi J., Masson P., Vincent J. P. Monoclonal antibodies allow precipitation of esterasic but not peptidasic activities associated with butyrylcholinesterase. J. Neurochem. 55:1990;750-755.
17. Kunkel T. A. Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA. 82:1985;488-492.
18. Fournier D., Mutero A., Rungger D. DrosophilaXenopus. Eur. J. Biochem. 203:1992;513-519.
19. O'Reilly D., Miller L. K., Luckow V. A. Baculovirus Expression Vectors: A Laboratory Manual. 1992;Freeman, New York.
20. Ellman G. L., Courtney K. D., Andres V., Featherstone R. M. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 7:1961;88-95.
21. Levy D., Ashani Y. Synthesis andin vitro. Biochem. Pharmacol. 35:1986;1079-1085.
22. Slack J. M., Kuzio J., Faulkner P. Characterization of v-cath, a cathepsin L-like proteinase expressed by the baculovirusAutographa californica. J. Gen. Virol. 76:1995;1091-1098.
23. Ohkawa T., Majima K., Maeda S. A cysteine protease encoded by the baculovirusBombyx mori. J. Virol. 68:1994;6619-6625.
24. Dwyer J. L. Process purificationin. Characterization and Stabilization" 1993;Humana Press, Clifton. p. 533-572.
25. Franks F. Storage stabilization of proteins. Protein Biotechnology, Isolation, Characterization and Stabilization. 1993;Humana Press, Clifton. p. 489-531.