Stability of protease in organic solvent: Structural identification by solid-state NMR of lyophilized papain before and after 1-propanol treatment and the corresponding enzymatic activities

Biotechnology and Bioengineering

Volumn 93, Issue 5, 2006, Pages 928-933

  Matsubara, Teruhiko ^a   Fujita, Risa ^b   Sugiyama, Shigeru ^b   Kawashiro, Katsuhiro ^b  

^a KEIO UNIVERSITY   (Japan)

^b UNIVERSITY OF TOKUSHIMA   (Japan)

Author keywords

CPMAS solid state NMR; Lyophilization; Organic solvent; Papain; Structural stability

Indexed keywords

ALCOHOLS; ENZYME KINETICS; ENZYMES; ESTERIFICATION; MOLECULAR STRUCTURE; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY;

CPMAS SOLID-STATE NMR; LYOPHILIZATION; PAPAIN; STRUCTURAL STABILITY;

ORGANIC SOLVENTS;

CARBON 13; ORGANIC SOLVENT; PAPAIN; PROPANOL; PROTEINASE;

ARTICLE; CIRCULAR DICHROISM; ENZYME ACTIVITY; FREEZE DRYING; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; SOLID STATE;

1-PROPANOL; AMIDES; CIRCULAR DICHROISM; DIPEPTIDES; ENZYME STABILITY; ESTERIFICATION; FREEZE DRYING; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; ORGANIC CHEMICALS; PAPAIN; PROTEIN DENATURATION; PROTEIN STRUCTURE, SECONDARY; SOLVENTS; TEMPERATURE;

EID: 33646041627     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.20791     Document Type: Article

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