Organic solvent binding to crystalline subtilisin in mostly aqueous media and in the neat solvents

Biochemical and Biophysical Research Communications

Volumn 248, Issue 2, 1998, Pages 273-277

  Schmitke, Jennifer L ^a   Stern, Lawrence J ^a   Klibanov, Alexander M ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACETONITRILE; DIOXANE; ORGANIC SOLVENT; SUBTILISIN;

ARTICLE; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; PRIORITY JOURNAL;

EID: 0032551728     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.1998.8937     Document Type: Article

References (24)

1. Fitzpatrick P. A., Steinmetz A. C. U., Ringe D., Klibanov A. M. Proc. Natl. Acad. Sci. U.S.A. 90:1993;8653-8657.
2. Peisach E., Casebier D., Gallion S. L., Furth P., Petsko G. A., Hogan J. C. Jr., Ringe D. Science. 296:1995;66-69.
3. Allen K. N., Bellamacina C. R., Ding X., Jeffery C. J., Mattos C., Petsko G. A., Ringe D. J. Phys. Chem. 100:1996;2605-2611.
4. Mattos C., Ringe D. Nature Biotechnol. 14:1996;595-599.
5. Miranker A., Karplus M. Proteins: Struct., Funct., Genet. 11:1991;29-34.
6. Caflish A., Miranker A., Karplus M. J. Med. Chem. 36:1993;2142-2167.
7. Fitzptrick P. A., Ringe D., Klibanov A. M. Biochem. Biophys. Res. Commun. 198:1994;675-681.
8. Schmitke J. L., Stern L. J., Klibanov A. M. Proc. Natl. Acad. Sci. U.S.A. 94:1997;4250-4255.
9. Neidhart D. J., Petsko G. A. Protein Eng. 2:1988;271-276.
10. Otwinowski Z., Minor W. Meth. Enzymol. 276:1997;307-326.
11. Acta Cryst. D50:1994;760-763.
12. Brünger A. T., Kuriyan J., Karplus M. Science. 35:1987;458-460.
13. Jones T. A., Zou J. Y., Cowan S. W., Kjeldgaard M. Acta Cryst. A. 47:1991;110-119.
14. Brünger A. T. Nature. 355:1992;472-475.
15. Kleywegt G. J. ESF/CCP4 Newsletter. 32:1995.
16. Laskowski R. A., MacArthur M. W., Moss D. S., Thornton J. M. J. Appl. Cryst. 26:1993;283-291.
17. Luzzati V. Acta Cryst. 5:1952;802-810.
18. Kleywegt G. L., Jones T. A. Structure. 2:1994;1241-1258.
19. Kleywegt G. L., Brünger A. T. Structure. 4:1996;897-904.
20. Read R. J. Acta Cryst. A. 42:1986;140-149.
21. Joseph-McCarthy D., Federov A. A., Almo S. L. Protein Eng. 9:1996;773-780.
22. Liepinsh E., Otting G. Nature Biotechnol. 15:1997;264-268.
23. Schmitke, J. L. Stern, L. J. Klibanov, A. M. J. Am. Chem. Soc.
24. Connolly M. L. Science. 221:1983;709-712.