Engineering drought tolerance in plants: discovering and tailoring genes to unlock the future

Current Opinion in Biotechnology

Volumn 17, Issue 2, 2006, Pages 113-122

  Umezawa, Taishi ^a,b   Fujita, Miki ^b,c   Fujita, Yasunari ^d   Yamaguchi Shinozaki, Kazuko ^c,d,e   Shinozaki, Kazuo ^a,b,c  

^a RIKEN Center for Sustainable Resource Science   (Japan)

^b RIKEN TSUKUBA INSTITUTE   (Japan)

^c JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^d JAPAN INTERNATIONAL RESEARCH CENTER FOR AGRICULTURAL SCIENCES   (Japan)

^e UNIVERSITY OF TOKYO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AGRICULTURE; DROUGHT; GENETIC ENGINEERING;

DROUGHT TOLERANCE; GENE DISCOVERY; TRANSCRIPTIONAL REGULATION;

PLANTS (BOTANY);

TRANSCRIPTION FACTOR;

AGRICULTURAL PROCEDURES; DROUGHT STRESS; DROUGHT TOLERANCE; GENE EXPRESSION; GENETIC ANALYSIS; GENETIC ENGINEERING; GENOME; GENOMICS; MOLECULAR GENETICS; PLANT BIOTECHNOLOGY; PLANT GENETICS; PRIORITY JOURNAL; REGULATOR GENE; REVIEW; SIGNAL TRANSDUCTION; TRANSCRIPTION REGULATION; TRANSGENIC PLANT;

BIOTECHNOLOGY; DEHYDRATION; FORECASTING; GENETIC ENGINEERING; NATURAL DISASTERS; PLANTS, GENETICALLY MODIFIED;

AGRICULTURE;

EID: 33645924685     PISSN: 09581669     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.copbio.2006.02.002     Document Type: Review

References (84)

1. Boyer J.S. Plant productivity and environment. Science 218 (1982) 443-448
2. Ramachandra-Reddy A., Chaitanya K.V., and Vivekanandan M. Drought-induced responses of photosynthesis and antioxidant metabolism in higher plants. J Plant Physiol 161 (2004) 1189-1202
3. Flowers T.J. Improving crop salt tolerance. J Exp Bot 55 (2004) 307-319
4. Vinocur B., and Altman A. Recent advances in engineering plant tolerance to abiotic stress: achievements and limitations. Curr Opin Biotechnol 16 (2005) 123-132
5. Chaves M.M., and Oliveira M.M. Mechanisms underlying plant resistance to water deficits: prospects for water-saving agriculture. J Exp Bot 55 (2004) 2365-2384
6. Chen T.H., and Murata N. Enhancement of tolerance of abiotic stress by metabolic engineering of betaines and other compatible solutes. Curr Opin Plant Biol 5 (2002) 250-257
7. Nyyssola A., Kerovuo J., Kaukinen P., von Weymarn N., and Reinikainen T. Extreme halophiles synthesize betaine from glycine by methylation. J Biol Chem 275 (2000) 22196-22201
8. Waditee R., Tanaka Y., Aoki K., Hibino T., Jikuya H., Takano J., Takabe T., and Takabe T. Isolation and functional characterization of N-methyltransferases that catalyze betaine synthesis from glycine in a halotolerant photosynthetic organism Aphanothece halophytica. J Biol Chem 278 (2003) 4932-4942
9. Waditee R., Bhuiyan M.N., Rai V., Aoki K., Tanaka Y., Hibino T., Suzuki S., Takano J., Jagendorf A.T., Takabe T., et al. Genes for direct methylation of glycine provide high levels of glycinebetaine and abiotic-stress tolerance in Synechococcus and Arabidopsis. Proc Natl Acad Sci USA 102 (2005) 1318-1323. The authors show that the coexpression of N-methyltransferase genes, which catalyze the biosynthetic pathway of betaine from glycine, resulted in accumulation of a significant amount of betaine and conferred salt tolerance to Arabidopsis plants.
10. Bartels D., and Sunkar R. Drought and salt tolerance in plants. Crit Rev Plant Sci 24 (2005) 23-58. This recent comprehensive review summarizes the molecular mechanism of drought and salt tolerance in plants, and provides an excellent perspective describing the components in the stress-responsive signaling pathway.
11. Iuchi S., Kobayashi M., Taji T., Naramoto M., Seki M., Kato T., Tabata S., Kakubari Y., Yamaguchi-Shinozaki K., and Shinozaki K. Regulation of drought tolerance by gene manipulation of 9-cis-epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in Arabidopsis. Plant J 27 (2001) 325-333
12. Saito S., Hirai N., Matsumoto C., Ohigashi H., Ohta D., Sakata K., and Mizutani M. Arabidopsis CYP707As encode (+)-abscisic acid 8′-hydroxylase, a key enzyme in the oxidative catabolism of abscisic acid. Plant Physiol 134 (2004) 1439-1449. The authors identify and characterize a small P450 family that encodes ABA 8′-hydroxylases, which play a key role in ABA catabolism.
13. Kushiro T., Okamoto M., Nakabayashi K., Yamagishi K., Kitamura S., Asami T., Hirai N., Koshiba T., Kamiya Y., and Nambara E. The Arabidopsis cytochrome P450 CYP707A encodes ABA 8′-hydroxylases: key enzymes in ABA catabolism. EMBO J 23 (2004) 1647-1656. The authors use genomic approaches to identify ABA 8′ hydroxylase genes (CYP707A1-4) in Arabidopsis. Expression analysis of the four genes reveals that CYP707A2 is responsible for the rapid decrease in ABA content in imbibed seeds. Knockout mutants of CYP707A2 have higher dormancy and higher ABA content than wild-type seeds.
14. Umezawa T, Okamoto M, Kushiro T, Nambara E, Oono Y, Seki M, Kobayashi M, Koshiba T, Kamiya Y, Shinozaki K: CYP707A3, a major ABA 8′-hydroxylase involved in dehydration and rehydration response in Arabidopsis thaliana. Plant J 2006: in press.
15. Goyal K., Walton L.J., and Tunnacliffe A. LEA proteins prevent protein aggregation due to water stress. Biochem J 388 (2005) 151-157
16. Wise M.J. LEAping to conclusions: a computational reanalysis of late embryogenesis abundant proteins and their possible roles. BMC Bioinformatics 4 (2003) 52
17. Wise M.J., and Tunnacliffe A. POPP the question: what do LEA proteins do?. Trends Plant Sci 9 (2004) 13-17
18. Oksman-Caldentey K.M., and Saito K. Integrating genomics and metabolomics for engineering plant metabolic pathways. Curr Opin Biotechnol 16 (2005) 174-179
19. Seki M., Narusaka M., Abe H., Kasuga M., Yamaguchi-Shinozaki K., Carninci P., Hayashizaki Y., and Shinozaki K. Monitoring the expression pattern of 1300 Arabidopsis genes under drought and cold stresses by using a full-length cDNA microarray. Plant Cell 13 (2001) 61-72
20. Fowler S., and Thomashow M.F. Arabidopsis transcriptome profiling indicates that multiple regulatory pathways are activated during cold acclimation in addition to the CBF cold response pathway. Plant Cell 14 (2002) 1675-1690
21. Maruyama K., Sakuma Y., Kasuga M., Ito Y., Seki M., Goda H., Shimada Y., Yoshida S., Shinozaki K., and Yamaguchi-Shinozaki K. Identification of cold-inducible downstream genes of the Arabidopsis DREB1A/CBF3 transcriptional factor using two microarray systems. Plant J 38 (2004) 982-993
22. Kasuga M., Liu Q., Miura S., Yamaguchi S., and Shinozaki K. Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducible transcription factor. Nat Biotechnol 17 (1999) 287-291
23. Aharoni A., Dixit S., Jetter R., Thoenes E., van Arkel G., and Pereira A. The SHINE clade of AP2 domain transcription factors activates wax biosynthesis, alters cuticle properties, and confers drought tolerance when overexpressed in Arabidopsis. Plant Cell 16 (2004) 2463-2480
24. Zhang J.Y., Broeckling C.D., Blancaflor E.B., Sledge M.K., Sumner L.W., and Wang Z.Y. Overexpression of WXP1, a putative Medicago truncatula AP2 domain-containing transcription factor gene, increases cuticular wax accumulation and enhances drought tolerance in transgenic alfalfa (Medicago sativa). Plant J 42 (2005) 689-707
25. Zhang J.Z., Creelman R.A., and Zhu J.K. From laboratory to field. Using information from Arabidopsis to engineer salt, cold, and drought tolerance in crops. Plant Physiol 135 (2004) 615-621
26. Liu Q., Kasuga M., Sakuma Y., Abe H., Miura S., Yamaguchi-Shinozaki K., and Shinozaki K. Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell 10 (1998) 1391-1406
27. Fujita Y, Fujita M, Satoh R, Maruyama K, Parvez MM, Seki M, Hiratsu K, Ohme-Takagi M, Shinozaki K, Yamaguchi-Shinozaki K: AREB1 is a transcription activator of novel ABRE-dependent ABA-signaling that enhances drought stress tolerance in Arabidopsis, Plant Cell 2005, in press. The authors demonstrate two powerful tools - a constitutively active form and a dominant loss-of-function mutant with a repression domain - that are useful for dissecting important TFs that seem to be required for post-transcriptional modification. This approach is also useful in cases when phenotypic masking is expected owing to potential functional redundancy of TFs.
28. Devaux F., Marc P., Bouchoux C., Delaveau T., Hikkel I., Potier M.C., and Jacq C. An artificial transcription activator mimics the genome-wide properties of the yeast Pdr1 transcription factor. EMBO Rep 2 (2001) 493-498
29. Kagaya Y., Hobo T., Murata M., Ban A., and Hattori T. Abscisic acid-induced transcription is mediated by phosphorylation of an abscisic acid response element binding factor, TRAB1. Plant Cell 14 (2002) 3177-3189
30. Furihata T., Maruyama K., Fujita Y., Umezawa T., Yoshida R., Shinozaki K., and Yamaguchi-Shinozaki K. Abscisic acid-dependent multisite phosphorylation regulates the activity of a transcription activator AREB1. Proc Natl Acad Sci USA 103 (2006) 1988-1993
31. Zhang J.Z. Overexpression analysis of plant transcription factors. Curr Opin Plant Biol 6 (2003) 430-440. A recent excellent review focusing on the overexpression of plant TFs. This manuscript provides many valuable insights into the creation of useful TF overexpressors.
32. Cominelli E., Galbiati M., Vavasseur A., Conti L., Sala T., Vuylsteke M., Leonhardt N., Dellaporta S.L., and Tonelli C. A guard-cell-specific MYB transcription factor regulates stomatal movements and plant drought tolerance. Curr Biol 15 (2005) 1196-1200
33. Kasuga M., Miura S., Shinozaki K., and Yamaguchi-Shinozaki K. A combination of the Arabidopsis DREB1A gene and stress-inducible rd29A promoter improved drought- and low-temperature stress tolerance in tobacco by gene transfer. Plant Cell Physiol 45 (2004) 346-350
34. Schroeder J.I., Kwak J.M., and Allen G.J. Guard cell abscisic acid signalling and engineering drought hardiness in plants. Nature 410 (2001) 327-330
35. Hiratsu K., Matsui K., Koyama T., and Ohme-Takagi M. Dominant repression of target genes by chimeric repressors that include the EAR motif, a repression domain, in Arabidopsis. Plant J 34 (2003) 733-739. The authors established an interesting dominant negative strategy with a repression domain, converting a transcriptional activator to a repressor, as a means to minimize the effects of phenotypic masking owing to functional redundancy.
36. Fujita M., Fujita Y., Maruyama K., Seki M., Hiratsu K., Ohme-Takagi M., Tran L.S., Yamaguchi-Shinozaki K., and Shinozaki K. A dehydration-induced NAC protein, RD26, is involved in a novel ABA-dependent stress-signaling pathway. Plant J 39 (2004) 863-876. The first study to reveal an unknown function of a TF using a dominant negative strategy with a repression domain. The authors demonstrated the involvement of a NAC TF in a novel ABA-dependent stress signaling pathway.
37. Kubo M., Udagawa M., Nishikubo N., Horiguchi G., Yamaguchi M., Ito J., Mimura T., Fukuda H., and Demura T. Transcription switches for protoxylem and metaxylem vessel formation. Genes Dev 19 (2005) 1855-1860
38. Sakamoto H., Maruyama K., Sakuma Y., Meshi T., Iwabuchi M., Shinozaki K., and Yamaguchi-Shinozaki K. Arabidopsis Cys2/His2-type zinc-finger proteins function as transcription repressors under drought, cold, and high-salinity stress conditions. Plant Physiol 136 (2004) 2734-2746
39. Kim S.H., Hong J.K., Lee S.C., Sohn K.H., Jung H.W., and Hwang B.K. CAZFP1, Cys2/His2-type zinc-finger transcription factor gene functions as a pathogen-induced early-defense gene in Capsicum annuum. Plant Mol Biol 55 (2004) 883-904
40. Boudsocq M., and Lauriere C. Osmotic signaling in plants: multiple pathways mediated by emerging kinase families. Plant Physiol 138 (2005) 1185-1194. This review summarizes the relationship between protein phosphorylation and dephosphorylation events and osmotic signaling in plants. Protein kinases, including MAPKs, CDPKs, SnRK2s and SnRK3s, and protein phosphatases are well documented in this article.
41. Shinozaki K., Yamaguchi-Shinozaki K., and Seki M. Regulatory network of gene expression in the drought and cold stress responses. Curr Opin Plant Biol 6 (2003) 410-417
42. Chinnusamy V., Schumaker K., and Zhu J.K. Molecular genetic perspectives on cross-talk and specificity in abiotic stress signalling in plants. J Exp Bot 55 (2004) 225-236
43. Kovtun Y., Chiu W.L., Tena G., and Sheen J. Functional analysis of oxidative stress-activated mitogen-activated protein kinase cascade in plants. Proc Natl Acad Sci USA 97 (2000) 2940-2945
44. Shou H., Bordallo P., and Wang K. Expression of the Nicotiana protein kinase (NPK1) enhanced drought tolerance in transgenic maize. J Exp Bot 55 (2004) 1013-1019
45. Cutler S., Ghassemian M., Bonetta D., Cooney S., and McCourt P. A protein farnesyl transferase involved in abscisic acid signal transduction in Arabidopsis. Science 273 (1996) 1239-1241
46. Pei Z.M., Ghassemian M., Kwak C.M., McCourt P., and Schroeder J.I. Role of farnesyltransferase in ABA regulation of guard cell anion channels and plant water loss. Science 282 (1998) 287-290
47. Wang Y., Ying J., Kuzma M., Chalifoux M., Sample A., McArthur C., Uchacz T., Sarvas C., Wan J., Dennis D.T., et al. Molecular tailoring of farnesylation for plant drought tolerance and yield protection. Plant J 43 (2005) 413-424. This work demonstrates that conditional antisense downregulation of the α or β subunits of protein farnesyl transferase, which is involved in the ABA signaling pathway, enhances the drought tolerance of Arabidopsis or canola plants. The authors confirmed the tolerance in field tests, which demonstrated that seed yield, oil content and composition were maintained.
48. Davies J.P., Yildiz F.H., and Grossman A.R. Sac3, an Snf1-like serine/threonine kinase that positively and negatively regulates the responses of Chlamydomonas to sulfur limitation. Plant Cell 11 (1999) 1179-1190
49. Mikolajczyk M., Awotunde O.S., Muszynska G., Klessig D.F., and Dobrowolska G. Osmotic stress induces rapid activation of a salicylic acid-induced protein kinase and a homolog of protein kinase ASK1 in tobacco cells. Plant Cell 12 (2000) 165-178
50. Li J., Wang X.Q., Watson M.B., and Assmann S.M. Regulation of abscisic acid-induced stomatal closure and anion channels by guard cell AAPK kinase. Science 287 (2000) 300-303
51. Monks D.E., Aghoram K., Courtney P.D., DeWald D.B., and Dewey R.E. Hyperosmotic stress induces the rapid phosphorylation of a soybean phosphatidylinositol transfer protein homolog through activation of the protein kinases SPK1 and SPK2. Plant Cell 13 (2001) 1205-1219
52. Kobayashi Y., Yamamoto S., Minami H., Kagaya Y., and Hattori T. Differential activation of the rice sucrose nonfermenting 1-related protein kinase 2 family by hyperosmotic stress and abscisic acid. Plant Cell 16 (2004) 1163-1177
53. Boudsocq M., Barbier-Brygoo H., and Lauriere C. Identification of nine SNF1-related protein kinases 2 activated by hyperosmotic and saline stresses in Arabidopsis thaliana. J Biol Chem 279 (2004) 41758-41766
54. Mustilli A.C., Merlot S., Vavasseur A., Fenzi F., and Giraudat J. Arabidopsis OST1 protein kinase mediates the regulation of stomatal aperture by abscisic acid and acts upstream of reactive oxygen species production. Plant Cell 14 (2002) 3089-3099
55. Yoshida R., Hobo T., Ichimura K., Mizoguchi T., Takahashi F., Aronso J., Ecker J.R., and Shinozaki K. ABA-activated SnRK2 protein kinase is required for dehydration stress signaling in Arabidopsis. Plant Cell Physiol 43 (2002) 1473-1483
56. Yoshida R, Umezawa T, Mizoguchi T, Takahashi F, Takahashi S, Shinozaki K: The regulatory domain of SRK2E/OST1/SnRK2.6 interacts with ABI1 and integrates ABA and osmotic stress signals controlling stomatal closure in Arabidopsis. J Biol Chem 2006: in press.
57. Umezawa T., Yoshida R., Maruyama K., Yamaguchi-Shinozaki K., and Shinozaki K. SRK2C, a SNF1-related protein kinase 2, improves drought tolerance by controlling stress-responsive gene expression in Arabidopsis thaliana. Proc Natl Acad Sci USA 101 (2004) 17306-17311. The authors identified an osmotic-stress-activated protein kinase, SRK2C, and analyzed its functions in planta. Overexpression of SRK2C resulted in higher drought tolerance, which coincided with the upregulation of stress-responsive genes. This is the first functional analysis of osmotic-stress-activated SnRK2.
58. Albrecht V., Weinl S., Blazevic D., D'Angelo C., Batistic O., Kolukisaoglu U., Bock R., Schulz B., Harter K., and Kudla J. The calcium sensor CBL1 integrates plant responses to abiotic stresses. Plant J 36 (2003) 457-470
59. Cheong Y.H., Kim K.N., Pandey G.K., Gupta R., Grant J.J., and Luan S. CBL1, a calcium sensor that differentially regulates salt, drought, and cold responses in Arabidopsis. Plant Cell 15 (2003) 1833-1845
60. Gong D., Guo Y., Schumaker K.S., and Zhu J.K. The SOS3 family of calcium sensors and SOS2 family of protein kinases in Arabidopsis. Plant Physiol 134 (2004) 919-926. This review summarizes the SOS3 (CBL) family and SOS2 (CIPK) family in plants. Each function of these proteins is well described within the article.
61. Batistic O., and Kudla J. Integration and channeling of calcium signaling through the CBL calcium sensor/CIPK protein kinase network. Planta 219 (2004) 915-924. A review of the CBL (SOS3) and CIPK (SOS2) families in plants. The authors emphasize the importance of the CBL-CIPK combination for signal output.
62. Kolukisaoglu U., Weinl S., Blazevic D., Batistic O., and Kudla J. Calcium sensors and their interacting protein kinases: genomics of the Arabidopsis and rice CBL-CIPK signaling networks. Plant Physiol 134 (2004) 43-58
63. Hrabak E.M., Chan C.W., Gribskov M., Harper J.F., Choi J.H., Halford N., Kudla J., Luan S., Nimmo H.G., Sussman M.R., et al. The Arabidopsis CDPK-SnRK superfamily of protein kinases. Plant Physiol 132 (2003) 666-680
64. Gong D., Zhang C., Chen X., Gong Z., and Zhu J.K. Constitutive activation and transgenic evaluation of the function of an Arabidopsis PKS protein kinase. J Biol Chem 277 (2002) 42088-42096
65. Posas F., and Saito H. Osmotic activation of the HOG MAPK pathway via Ste11p MAPKKK: scaffold role of Pbs2p MAPKK. Science 276 (1997) 1702-1705
66. Park S.H., Zarrinpar A., and Lim W.A. Rewiring MAP kinase pathways using alternative scaffold assembly mechanisms. Science 299 (2003) 1061-1064
67. Nakagami H., Pitzschke A., and Hirt H. Emerging MAP kinase pathways in plant stress signaling. Trends Plant Sci 10 (2005) 339-346
68. Yamaguchi-Shinozaki K., and Shinozaki K. Organization of cis-acting regulatory elements in osmotic- and cold-stress-responsive promoters. Trends Plant Sci 10 (2005) 88-94. An excellent review article describing the regulatory networks in plants exposed to osmotic or cold stress, focusing on cis-acting elements and transcription factors. Several cis-acting elements and TFs are described and their functions illustrated.
69. Capell T., Bassie L., and Christou P. Modulation of the polyamine biosynthetic pathway in transgenic rice confers tolerance to drought stress. Proc Natl Acad Sci USA 101 (2004) 9909-9914
70. Kasukabe Y., He L., Nada K., Misawa S., Ihara I., and Tachibana S. Overexpression of spermidine synthase enhances tolerance to multiple environmental stresses and up-regulates the expression of various stress-regulated genes in transgenic Arabidopsis thaliana. Plant Cell Physiol 45 (2004) 712-722
71. Yamada M., Morishita H., Urano K., Shiozaki N., Yamaguchi-Shinozaki K., Shinozaki K., and Yoshiba Y. Effects of free proline accumulation in petunias under drought stress. J Exp Bot 56 (2005) 1975-1981
72. Cortina C., and Culiáñez-Maciá F. Tomato abiotic stress enhanced tolerance by trehalose biosynthesis. Plant Sci 169 (2005) 75-82
73. Chandra Babu R., Jingxian Z., Blumc A., David Hod T.-H., Wue R., and Nguyenf H.T. HVA1, a LEA gene from barley confers dehydration tolerance in transgenic rice (Oryza sativa L.) via cell membrane protection. Plant Sci 166 (2004) 855-862
74. Park B.-J., Liu Z., Kanno A., and Kameya T. Genetic improvement of chinese cabbage for salt and drought tolerance by constitutive expression of a B. napus LEA gene. Plant Sci 169 (2005) 553-558
75. De Block M., Verduyn C., De Brouwer D., and Cornelissen M. Poly(ADP-ribose) polymerase in plants affects energy homeostasis, cell death and stress tolerance. Plant J 41 (2005) 95-106
76. Laporte M.M., Shen B., and Tarczynski M.C. Engineering for drought avoidance: expression of maize NADP-malic enzyme in tobacco results in altered stomatal function. J Exp Bot 53 (2002) 699-705
77. Wang W., Vinocur B., and Altman A. Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta 218 (2003) 1-14
78. Qin F., Sakuma Y., Li J., Liu Q., Li Y.Q., Shinozaki K., and Yamaguchi-Shinozaki K. Cloning and functional analysis of a novel DREB1/CBF transcription factor involved in cold-responsive gene expression in Zea mays L. Plant Cell Physiol 45 (2004) 1042-1052
79. Novillo F., Alonso J.M., Ecker J.R., and Salinas J. CBF2/DREB1C is a negative regulator of CBF1/DREB1B and CBF3/DREB1A expression and plays a central role in stress tolerance in Arabidopsis. Proc Natl Acad Sci USA 101 (2004) 3985-3990
80. Pellegrineschi A., Reynolds M., Pacheco M., Brito R.M., Almeraya R., Yamaguchi-Shinozaki K., and Hoisington D. Stress-induced expression in wheat of the Arabidopsis thaliana DREB1A gene delays water stress symptoms under greenhouse conditions. Genome 47 (2004) 493-500
81. Oh S.J., Song S.I., Kim Y.S., Jang H.J., Kim S.Y., Kim M., Kim Y.K., Nahm B.H., and Kim J.K. Arabidopsis CBF3/DREB1A and ABF3 in transgenic rice increased tolerance to abiotic stress without stunting growth. Plant Physiol 138 (2005) 341-351
82. Kim S., Kang J.Y., Cho D.I., Park J.H., and Kim S.Y. ABF2, an ABRE-binding bZIP factor, is an essential component of glucose signaling and its overexpression affects multiple stress tolerance. Plant J 40 (2004) 75-87
83. Tran L.S., Nakashima K., Sakuma Y., Simpson S.D., Fujita Y., Maruyama K., Fujita M., Seki M., Shinozaki K., and Yamaguchi-Shinozaki K. Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought-responsive cis-element in the early responsive to dehydration stress 1 promoter. Plant Cell 16 (2004) 2481-2498
84. Yan J., He C., Wang J., Mao Z., Holaday S.A., Allen R.D., and Zhang H. Overexpression of the Arabidopsis 14-3-3 protein GF14λ in cotton leads to a 'stay-green' phenotype and improves stress tolerance under moderate drought conditions. Plant Cell Physiol 45 (2004) 1007-1014