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Volumn 282, Issue 5387, 1998, Pages 287-290

Role of farnesyltransferase in ABA regulation of guard cell anion channels and plant water loss

Author keywords

[No Author keywords available]

Indexed keywords

ABSCISIC ACID; ANION CHANNEL; FARNESYL TRANS TRANSFERASE;

EID: 0032500887     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.282.5387.287     Document Type: Article
Times cited : (324)

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    • -1. Subsequently, the indicated concentrations of ABA, or 2 μM HFPA or 5 μM manumycin, or ABA and 2 μM HFPA or 5 μM manumycin were added to the solutions to assay for stomatal closing. After treatments for 2 hours, stomatal apertures were observed with a digital video camera attached to an inverted microscope. Stomatal density was not affected in era1-2.
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    • In addition, ABA activation of anion-channel currents was also analyzed at 1 and 50 μM ABA (n = 23 and 28 cells for WT and era1-2, respectively). Activation of anion currents was also potentiated in era1-2 compared with WT at 1 μM ABA, whereas at 50 μM ABA both WT and era1-2 responses were similar.
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    • 2 seeds were screened for ABA insensitivity to select for abi1 or abi2. In the next generation seeds were screened for ABA supersensitivity (era1/era1). Supersensitive seeds were advanced to the next generation. Homozygous double mutants were identified by PCR amplification with primers of 5′-GATATCTCCGCCGGAGAT-3′ and 5′-CCATTCCACTGAATCACTTT-3′ for abi1-1, and 5′-CATCATCTGCTATGGCAGG-3′ and 5′-CCGGAGCATGAGCCACAG-3′ for abi2-1, as described (19). The era1-2 deletion was verified by Southern (DNA) blot with ERA1 cDNA as a probe. The era1/abi1 mutant was further verified by back crosses to both parents to confirm genetically that it was a double mutant.
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    • C. M. Kwak, data not shown. ABA inhibition of seed germination was analyzed as described (17). Germination of seeds was defined as positive when a radical tip had fully penetrated the seed coat (n = 50 per condition). Each experiment (n = 25) included conditions comparing the indicated lines at multiple ABA concentrations.
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    • note
    • We thank T. A. Theobald, V. M. Baizabal-Aguirre, K. Kuchitsu, and X.-F. Cheng for assistance, and E. J. Kim, G. J. Allen, W. R. Schafer, N. M. Crawford, M. F. Yanofsky, and R. Y. Hampton for discussions and reading of the manuscript. Supported by National Science Foundation (MCB-9506191) and Department of Energy grants (94-ER20148) to J.I.S. and by a Natural Science and Engineering Research Council of Canada grant to P.M.


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