A protein farnesyl transferase involved in abscisic acid signal transduction in Arabidopsis

Science

Volumn 273, Issue 5279, 1996, Pages 1239-1241

  Cutler, Sean ^a,b   Ghassemian, Majid ^a   Bonetta, Dario ^a   Cooney, Sarah ^a   McCourt, Peter ^a  

^a UNIVERSITY OF TORONTO   (Canada)

^b GEOPHYSICAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ABSCISIC ACID; PROTEIN FARNESYLTRANSFERASE;

ARABIDOPSIS; ARTICLE; GENE INSERTION; GENE MUTATION; NONHUMAN; PLANT GROWTH; PLANT SEED; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN MODIFICATION; SIGNAL TRANSDUCTION; STRESS;

ARABIDOPSIS; ARABIDOPSIS THALIANA; EMBRYOPHYTA; MAMMALIA;

EID: 0029839761     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.273.5279.1239     Document Type: Article

References (32)

1. J. A. D. Zeevaart and R. A. Creelman, Annu. Rev. Plant Physiol. Plant Mol. Biol. 39, 439 (1988); P. M. Chandler and M. Robertson, ibid. 45, 113 (1994).
2. J. A. D. Zeevaart and R. A. Creelman, Annu. Rev. Plant Physiol. Plant Mol. Biol. 39, 439 (1988); P. M. Chandler and M. Robertson, ibid. 45, 113 (1994).
3. K. Skriver and J. Mundy, Plant Cell 2, 503 (1990).
4. M. R. Blatt, G. Thiel, D. R. Trenthan, Nature 346, 766 (1990); S. Gilroy, N. D. Read, A. J. Trewavas, ibid. 346, 769 (1990); J. I. Schroeder and S. Hagiwara, Proc. Natl. Acad. Sci. U.S.A. 87, 9305 (1990); H. R. Irving, C. A. Gehring, R. W. Parish, ibid. 89, 1790 (1992).
5. M. R. Blatt, G. Thiel, D. R. Trenthan, Nature 346, 766 (1990); S. Gilroy, N. D. Read, A. J. Trewavas, ibid. 346, 769 (1990); J. I. Schroeder and S. Hagiwara, Proc. Natl. Acad. Sci. U.S.A. 87, 9305 (1990); H. R. Irving, C. A. Gehring, R. W. Parish, ibid. 89, 1790 (1992).
6. M. R. Blatt, G. Thiel, D. R. Trenthan, Nature 346, 766 (1990); S. Gilroy, N. D. Read, A. J. Trewavas, ibid. 346, 769 (1990); J. I. Schroeder and S. Hagiwara, Proc. Natl. Acad. Sci. U.S.A. 87, 9305 (1990); H. R. Irving, C. A. Gehring, R. W. Parish, ibid. 89, 1790 (1992).
7. M. R. Blatt, G. Thiel, D. R. Trenthan, Nature 346, 766 (1990); S. Gilroy, N. D. Read, A. J. Trewavas, ibid. 346, 769 (1990); J. I. Schroeder and S. Hagiwara, Proc. Natl. Acad. Sci. U.S.A. 87, 9305 (1990); H. R. Irving, C. A. Gehring, R. W. Parish, ibid. 89, 1790 (1992).
8. M. Koornneef and C. M. Karssen, in Arabidopsis, E. Meyerowitz and C. Somerville, Eds. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1994), pp. 313-334; R. R. Finkelstein, Plant J. 5, 765 (1994).
9. M. Koornneef and C. M. Karssen, in Arabidopsis, E. Meyerowitz and C. Somerville, Eds. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1994), pp. 313-334; R. R. Finkelstein, Plant J. 5, 765 (1994).
10. J. Leung et al., Science 264, 1448 (1994); K. Meyer, M. P. Leube, E. Grill, ibid., p. 1452.
11. J. Leung et al., Science 264, 1448 (1994); K. Meyer, M. P. Leube, E. Grill, ibid., p. 1452.
12. F. Parcy et al., Plant Cell. 6, 1567 (1994).
13. Wild-type seeds show a progressive inhibition of germination with increasing concentrations of ABA, with full inhibition occurring above 1.2 μM. An ABA concentration of 0.3 μM was chosen for screening mutagenized seed populations because this concentration of hormone had no inhibitory effect on wild-type germination. From about 110,000 fast-neutron and 59,000 T-DNA-mutagenized seed pools, 1800 putative mutant lines were identified. In the next generation, 22 lines gave good germination with minimal media and clear supersensitivity to 0.3 μM ABA. Of these, five neutron-induced and two T-DNA-induced mutant lines showed recessive Mendelian inheritance with respect to ABA supersensitivity and defined the three complementation groups era 1, era2, and era3 (9).
14. J. M. Baskin and C. C. Baskin, Can. J. Bot. 50, 277 (1971).
15. S. Cutler, M. Ghassemian, D. Bonetta, S. Cooney, P. McCourt, data not shown.
16. 2 parents showed absolute linkage of the T-DNA insertion and the ABA-supersensitive phenotype. Zero recombinants indicate with 95% probability that the T-DNA insertion is within 1.3 map units of the Era 1 gene.
17. Genomic Southern blots of era1-1 DNA digested with Eco RI and probed with right-border (RB) T-DNA produced three bands (13, 7.0, and 8 kb) that could be accounted for by a contiguous double insertion of T-DNA in which one of the copies has been inverted to give two flanking RB regions attached to plant genomic DNA. Subsequent analysis with other restriction enzymes verified that the 7- and 8-kb bands contained the insertion points of T-DNA and flanking plant DNA. DNA pools enriched for these fragments were ligated to the λ-ZAPII arms according to the manufacturer's instructions (Stratagene) and five positive plaques were identified that hybridized with the RB probe. Two plasmids (pSC10 and pSC11), each containing about 1.2 kb of T-DNA attached to different flanking regions, detected a single Eco RI 1.5-kb fragment in wild-type DNA by Southern analysis. Both clones therefore contain genomic sequences that are contiguous in wild-type DNA, indicating that the RB fragments in the era1-1 mutant represent the ends of a single-site T-DNA insertion. pSC10 was used as a probe to screen an Arabidopsis CDNA library, PRL2 λ-ZipLox (ABRC, stock CD4-7); and five positive cDNAs were identified and sequenced. The longest cDNA, pZL51 (1.45 kb), was used to screen Columbia (λ-ZAPII, Stratagene) and Lansberg genomic (λ-FIX, ABRC stock CD4-8) libraries and four positive clones were identified. One clone spanning 6 kb and encompassing the entire pZL51 clone was completely sequenced. A larger genomic insert (14 kb) was used to size deletions in the fast-neutron mutants (era1-2 and era 1-3).
18. B. W. Shirley, S. Hanley, H. M. Goodman, Plant Cell 4, 333 (1992).
19. L. E. Goodman, C. M. Perou, A. Fujiyama, F. Tamanoi, Yeast 4, 271 (1988); W-J. Chen, D. A. Anders, J. L. Goldstein, D. W. Russell, M. S. Brown, Cell 66, 327 (1991); Z. Yang, C. L. Cramer, J. C. Watson, Plant Physiol. 101, 667 (1993).
20. L. E. Goodman, C. M. Perou, A. Fujiyama, F. Tamanoi, Yeast 4, 271 (1988); W-J. Chen, D. A. Anders, J. L. Goldstein, D. W. Russell, M. S. Brown, Cell 66, 327 (1991); Z. Yang, C. L. Cramer, J. C. Watson, Plant Physiol. 101, 667 (1993).
21. L. E. Goodman, C. M. Perou, A. Fujiyama, F. Tamanoi, Yeast 4, 271 (1988); W-J. Chen, D. A. Anders, J. L. Goldstein, D. W. Russell, M. S. Brown, Cell 66, 327 (1991); Z. Yang, C. L. Cramer, J. C. Watson, Plant Physiol. 101, 667 (1993).
22. W. R. Schafer and J. Rine, Annu. Rev. Genet. 30, 209 (1992).
23. 3Hance (New England Nuclear), and exposed to Kodak X-OMAT AR film at - 70°C for 4 days.
24. 3Hance (New England Nuclear), and exposed to Kodak X-OMAT AR film at - 70°C for 4 days.
25. J. Hancock, H. Paterson, C. Marshall, Cell 43, 133 (1990); S. Leevers, H. F. Paterson, C. J. Marshall, Nature 369, 411 (1994); D. Stokoe, S. G. Macdonald, K, Cadwallader, M. Symons, J. F. Hancock, Science 264, 1463 (1994); P. J. Casey, Biochem. Soc. Trans. 23, 161 (1995).
26. J. Hancock, H. Paterson, C. Marshall, Cell 43, 133 (1990); S. Leevers, H. F. Paterson, C. J. Marshall, Nature 369, 411 (1994); D. Stokoe, S. G. Macdonald, K, Cadwallader, M. Symons, J. F. Hancock, Science 264, 1463 (1994); P. J. Casey, Biochem. Soc. Trans. 23, 161 (1995).
27. J. Hancock, H. Paterson, C. Marshall, Cell 43, 133 (1990); S. Leevers, H. F. Paterson, C. J. Marshall, Nature 369, 411 (1994); D. Stokoe, S. G. Macdonald, K, Cadwallader, M. Symons, J. F. Hancock, Science 264, 1463 (1994); P. J. Casey, Biochem. Soc. Trans. 23, 161 (1995).
28. J. Hancock, H. Paterson, C. Marshall, Cell 43, 133 (1990); S. Leevers, H. F. Paterson, C. J. Marshall, Nature 369, 411 (1994); D. Stokoe, S. G. Macdonald, K, Cadwallader, M. Symons, J. F. Hancock, Science 264, 1463 (1994); P. J. Casey, Biochem. Soc. Trans. 23, 161 (1995).
29. P. C. Sternweis, Curr. Opin. Cell Biol. 6, 198 (1994).
30. J. Inglese, W. J. Koch, M. G. Caron, R. J. Lefkowitz, Nature 359, 147 (1992).
31. Abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G. Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val: W, Trp; and Y. Tyr.
32. We thank K. Keith for thoughtful discussion and extensive contributions in the writing of this paper. We thank S. Sarkar for critical reading of the manuscript and D. Skalamera for technical assistance with photography. Many of the strains and DNA libraries used were provided by the Arabidopsis Stock Center at Ohio State University, which is supported by NSF. Support was provided by a Natural Sciences and Engineering Research Council of Canada grant to P.M.