False-positive results in a recombinant severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid-based western blot assay were rectified by the use of two subunits (S1 and S2) of spike for detection of antibody to SARS-CoV

Clinical and Vaccine Immunology

Volumn 13, Issue 3, 2006, Pages 409-414

  Maache, Mimoun ^a   Komurian Pradel, Florence ^a   Rajoharison, Alain ^a   Perret, Magali ^a   Berland, Jean Luc ^a   Pouzol, Stéphane ^a   Bagnaud, Audrey ^a   Duverger, Blandine ^a   Xu, Jianguo ^b   Osuna, Antonio ^c   Paranhos Baccalà, Glaucia ^a  

^a BIOMÉRIEUX   (France)

^b China CDC   (China)

^c UNIVERSITY OF GRANADA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

NUCLEOCAPSID PROTEIN; NUCLEOCAPSID PROTEIN N; PROTEIN S 1; PROTEIN S II; UNCLASSIFIED DRUG; VIRUS ANTIBODY; VIRUS ANTIGEN;

ANTIBODY DETECTION; ARTICLE; CONTROLLED STUDY; CROSS REACTION; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; ESCHERICHIA COLI; ESCHERICHIA COLI BL21; HUMAN; HUMAN CELL; PRIORITY JOURNAL; SARS CORONAVIRUS; SERODIAGNOSIS; SEVERE ACUTE RESPIRATORY SYNDROME; VALIDATION PROCESS; VIRUS NUCLEOCAPSID; VIRUS SPIKE; WESTERN BLOTTING;

ANTIBODIES, VIRAL; BLOTTING, WESTERN; FALSE POSITIVE REACTIONS; HUMANS; MEMBRANE GLYCOPROTEINS; NUCLEOCAPSID PROTEINS; PROTEIN SUBUNITS; RECOMBINANT PROTEINS; SARS VIRUS; SEVERE ACUTE RESPIRATORY SYNDROME; VIRAL ENVELOPE PROTEINS;

EID: 33645547094     PISSN: 15566811     EISSN: None     Source Type: Journal    
DOI: 10.1128/CVI.13.3.409-414.2006     Document Type: Article

References (37)

1. Ai, X. R., H. X. You, Y. Y. Kong, G. Z. Yang, Y. Z. Zhang, Y. Wang, and X. F. Wu. 2004. Expression, purification and sublocalization of SARS-CoV nucleocapsid protein in insect cells. Acta Biochim. Biophys. Sin. 36:754-758.
2. Bi, S., E. Qin, Q. Zhu, M. Yu, B. Fan, G. Chang, B. Si, Z. Zhenpeng, Z. Haiqing, Z. Yilin, Z. Hui, Z. Jun, Z. Xiuqing, L. Bin, Z. Changqing, L. Songgang, T. Xuehai, L. Siqi, D. Wei, W. Jun, K. W. Gane, Y. Jun, W. Jian, Z. Qingyu, and H. Yang. 2003. Complete genome sequences of the SARS-CoV: the BJ Group (Isolates BJ01-BJ04). Genomics Proteomics Bioinformatics 1:180-192.
3. Cavanagh, D. 1995. The coronavirus surface glycoprotein. In S. G. Siddell, Jr. (ed.), The Coronaviridae. Plenum Press, New York, N.Y.
4. Che, X. Y., W. Hao, L. W. Qiu, Y. X. Pan, Z. Y. Liao, H. Xu., J. J. Chen, J. L. Hou, P. C. Woo, and S. K. Lau. 2003. Antibody response of patients with severe acute respiratory syndrome (SARS) to nucleocapsid antigen of SARS-associated coronavirus. Di Yi Jun Yi Da Xue Xue Bao 23:640-642.
5. Drosten, C., S. Gunther, W. Preiser, S. V. Werf, H. R. Brodt, S. Becker, H. Rabenau, M. Panning, L. Kesnikova, R. A. Fouchier, A. Berger, A. M. Burguière, J. Cinatl, M. Eickmann, N. Escriou, K. Grywna, S. Kramme, J. C. Manuguerra, S. Müller, V. Rickerts, M. Stürmer, S. Vieth, H. D. Klenk, and H. W. Doerr. 2003. Identification of a novel coronavirus in patients with severe acute respiratory syndrome. N. Engl. J. Med. 348:1967-1976.
6. Fiscus, S. A., and Y. A. Teramoto. 1987. Antigenic comparison of feline coronavirus isolates: evidence for markedly different peplomer glycoproteins J. Virol. 61:2607-2613.
7. Gallagher, T. M., and M. J. Buchmeier. 2001. Coronavirus spike proteins in viral entry and pathogenesis. Virology 279:371-374.
8. Guan, M., H. Y. Chen, P. H. Tan, S. Shen, P. Goh, Y. Tan, P. H. Pang, Y. Lu, P. Y. Fong, and D. Chin. 2004. Use of viral lysate antigen combined with recombinant protein in Western immunoblot assay as confirmatory test for serodiagnosis of severe acute respiratory syndrome. Clin. Diagn. Lab. Immunol. 11:1148-1153.
9. Han, D. P., H. G. Kim, Y. B. Kim, L. L. Poon, and M. W. Cho. 2004. Development of a safe neutralization assay for SARS-CoV and characterization of S-glycoprotein. Virology 326:140-149.
10. He, Y., Y. Zhou, H. Wu, B. Luo, J. Chen, W. Li, and S. Jiang. 2004. Identification of immunodominant sites on the spike protein of severe acute respiratory syndrome (SARS) coronavirus: implication for developing SARS diagnostics and vaccines. J. Immunol. 173:4050-4057.
11. Ho, T. Y., S. L. Wu, S. E. Cheng, Y. C. Wei, S. P. Huang, and C. Y. Hsiang. 2004. Antigenicity and receptor-binding ability of recombinant SARS coronavirus spike protein. Biochem. Biophys. Res. Commun. 313:938-947.
12. Hofmann, H., K. Hattermann, A. Marzi, T. Gramberg, M. Geier, M. Krumbiegel, S. Kuate, K. Uberla, M. Niedrig, and S. Pohlmann. 2004. S protein of severe acute respiratory syndrome-associated coronavirus mediates entry into hepatoma cell lines and is targeted by neutralizing antibodies in infected patients. J. Virol. 78:6134-6142.
13. Holmes, K. V. 2003. SARS coronavirus: a new challenge for prevention and therapy. J. Clin. Investig. 111:1605-1609.
14. Holmes, K. V. 2003. SARS-associatcd coronavirus. N. Engl. J. Med. 348:1948-1951.
15. Holmes, K. V., and L. Enjuanes. 2003. Virology: the SARS coronavirus: a postgenomic era. Science 300:1377-1378.
16. Huang, L. R., C. M. Chiu, S. H. Yeh, W. H. Huang, P. R. Hsueh, W. Z. Yang, J. Y. Yang, I. J. Su, S. C. Chang, and P. J. Chen. 2004. Evaluation of antibody responses against SARS coronaviral nucleocapsid or spike proteins by immunoblotting or ELISA. 73:338-346.
17. Ksiazek, T. G., D. Erdman, C. S. Goldsmith, S. R. Zaki, T. Peret, S. Emery, S. Tong, C. Urbani, J. A. Comer, W. Lim, P. E. Rollin, S. F. Dowell, A. E. Ling, C. D. Humphrey, W. J. Shieh, J. Guarner, C. D. Paddock, P. Rota, B. Fields, J. DeRisi, J. Y. Yang, N. Cox, J. M. Hughes, J. W. LeDuc, W. J. Bellini, and L. J. Anderson. 2003. A novel coronavirus associated with severe acute respiratory syndrome. N. Engl. J. Med. 348:1953-1966.
18. Lu, L., I. Manopo, B. P. Leung, H. H. Chng, A. E. Ling, L. L. Chee, E. E. Ooi, S. W. Chan, and J. Kwang. 2004. Immunological characterization of the spike protein of the severe acute respiratory syndrome coronavirus. J. Clin. Microbiol. 42:1570-1576.
19. Marra, M. A., S. J. Jones, C. R. Astell, R. A. Holt, W. A. Brooks, Y. S. N. Butterfield, J. Khattra, J. K. Asano, S. A. Barber, S. Y. Chan, A. Cloutier, S. M. Coughlin, D. Freeman, N. Girn, O. L. Griffith, S. R. Leach, M. Mayo, H. McDonald, S. B. Montgomery, P. K. Pandoh, A. S. Petrescu, A. G. Robertson, J. E. Schein, A. Siddiqui, D. E. Smailus, J. M. Stott, and R. L. Roper. 2003. The genome sequence of the SARS-associatcd coronavirus. Science 300:1399-1404.
20. Peiris, J. S., S. T. Lai, L. L. Poon, Y. Guan, L. Y. Yam, W. Lim, J. Nicholls, W. Yee, W. W. Yan, M. T. Cheung, V. C. Cheng, K. H. Chan, D. N. Tsang, R. W. Yung, K. Y. Yuen, and members of the SARS study group. 2003. Coronavirus as a possible cause of severe acute respiratory syndrome. Lancet 361:1319-1325.
21. Poon, L. L., O. K. Wong, K. H. Chan, W. Luk, K. Y. Yuen, and J. S. Peiris. 2003. Rapid diagnosis of a coronavirus associated with severe acute respiratory syndrome (SARS). Clin. Chem. 49:953-955.
22. Ren, Y., Z. Zhou, J. Liu, L. Lin, S. Li, H. Wang, J. Xia, Z. Zhao, J. Wen, C. Zhou, J. Wang, J. Yin, N. Xu, and S. Liu. 2003. A strategy for searching antigenic regions in the SARS-CoV spike protein. Genomics Proteomics Bioinformatics 1:207-215.
23. Rota, P. A., M. S. Oberste, S. S. Monroe, W. A. Nix, R. Campagnoli, J. P. Icenogle, et al. 2003. Characterization of a novel coronavirus associated with severe acute respiratory syndrome. Science 300:1394-1399.
24. Saif, L. J. 1993. Coronavirus immunogens. Vet. Microbiol. 37:285-297.
25. Shi, Y., Y. Yi, P. Li, T. Kuang, L. Li, M. Dong, M. Qingjun, and C. Cao. 2003. Diagnosis of severe acute respiratory syndrome (SARS) by detection of SARS coronavirus nucleocapsid antibodies in an antigen-capturing enzyme-linked immunosorbent assay. J. Clin. Microbiol. 41:5781-5782.
26. Surjit, M., L. Boping, J. Shahid, T. K. Vincent, and S. K. Lal. 2004. The SARS coronavirus nucleocapsid protein induces actin reorganization and apoptosis in COS-1 cells in the absence of growth factors. Biochem. J. 383:13-18.
27. Tan, Y. J., P. Y. Goh, B. C. Fielding, S. Shen, C. F. Chou, J. L. Fu, H. N. Leong, Y. S. Leo, E. E. Ooi, A. E. Ling, S. G. Lim, and W. J. Hong. 2004. Profile of antibody responses against SARS-coronavirus recombinant proteins and their potential use as diagnostic markers. Clin. Diagn. Lab. Immunol. 11:362-371.
28. Wege, H., A. Schliephake, H. Korner, E. Flory, and H. Wege. 1993. An immunodominant CD4+ T cell site on the nucleocapsid protein of murine coronavirus contributes to protection against encephalomyclitis. J. Gen. Virol. 74(Pt 7):1287-1294.
29. Wei, L., X. D. Wu, M. Shi, R. F. Yang, Y. Y. He, C. Bian, T. L. Shi, S. Yang, X. Zhu, W. Jiang, Y. X. Li, L. Yan, Y. Y. Ji, Y. Lin, G. Lin, L. Tian, J. Wang, H. X. Wang, Y. H. Xie, G. Pei, J. R. Wu, and B. Sun. 2005. Synthetic peptides derived from SARS coronavirus S protein with diagnostic and therapeutic potential. FEBS Lett. 579:2130-2136.
30. Woo, P. C., S. K. Lau, B. H. Wong, H. Tsoi, A. M. Fung, K. H. Chan, W. T. Hui, G. S. Kwan, K. P. Victoria, J. S. Peiris, R. B. Couch, and K. Yuen. 2004. False-positive results in a recombinant severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid enzyme-linked immunosorbent assay due to HCoV-OC43 and HCoV-229E rectified by Western blotting with recombinant SARS-CoV spike polypeptide. J. Clin. Microbiol. 42:5885-5888.
31. Woo, P. C., S. K. Lau, B. H. Wong, H. Tsoi, A. M. Fung, K. Chan, K. P. Victoria, J. S. Peiris, and K. Yuen. 2004. Detection of specific antibodies to severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein for serodiagnosis of SARS coronavirus pneumonia. J. Clin. Microbiol. 42:2306-2309.
32. Wu, X. D., B. Shang, R. F. Yang, H. Yu, Z. H. Ma, X. Shen, Y. Y. Ji, Y. Lin, Y. D. Wu, G. M. Lin, L. Tian, X. Q. Gan, S. Yang, W. H. Jiang, E. H. Dai, X. Y. Wang, H. L. Jiang, Y. H. Xie, X. L. Zhu, G. Pei, L. Li, J. R. Wu, and B. Sun. 2004. The spike protein of severe acute respiratory syndrome (SARS) is cleaved in virus infected Vero-E6 cells. Cell Res. 14:400-406.
33. Yu, H., Y. Yang, W. Zhang, Y. H. Xie, J. Qin, Y. Wang, H. B. Zheng, G. P. Zhao, S. Yang, and W. H. Jiang. 2003. Expression and purification of recombinant SARS coronavirus spike protein. Acta Biochim. Biophys. Sin. 35:774-778.
34. Yuxian, H., H. Lu, S. Pamela, Z. Yusen, and J. Shibo. 2005. Receptor-binding domain of severe acute respiratory syndrome coronavirus spike protein contains multiple conformation-dependent epitopes that induce highly potent neutralizing antibodies. J. Immunol. 174:4908-4915.
35. Zhang, H., G. Wang, J. Li, Y. Nie, X. Shi, G. Lian, W. Wang, X. Yin, Y. Zhao, X. Qu, M. Ding, and H. Deng. 2004. Identification of an antigenic determinant on the S2 domain of the severe acute respiratory syndrome coronavirus spike protein neutralizing antibodies J. Virol. 78:6938-6945.
36. Zhao, P., J. Cao, L. J. Zhao, Z. L. Qin, J. S. Ke, W. Pan, H. Ren, J. G. Yu, and Z. T. Qi. 2005. Immune responses against SARS-coronavirus nucleocapsid protein induced by DNA vaccine. Virology 331:128-135.
37. Zhu, M. S., Y. Pan, H. Q. Chen, Y. Shen, X. C. Wang, Y. J. Sun. 2004. Induction of SARS-nucleoprotein-spccific immune response by use of DNA vaccine. Immunol. Lett. 92:237-243.