The SARS coronavirus nucleocapsid protein induces actin reorganization and apoptosis in COS-1 cells in the absence of growth factors

Biochemical Journal

Volumn 383, Issue 1, 2004, Pages 13-18

  Surjit, Milan ^a   Liu, Boping ^b   Jameel, Shahid ^a   Chow, Vincent T K ^b   Lal, Sunil K ^a  

^a INTERNATIONAL CENTRE FOR GENETIC ENGINEERING AND BIOTECHNOLOGY ICGEB   (India)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Actin reorganization; Caspase; Cell signalling; Growth factor; Programmed cell death; SARS coronavirus nucleocapsid

Indexed keywords

CELLS; DIAGNOSIS; DISEASE CONTROL; GENES; PATIENT MONITORING; PROTEINS;

CORONAVIRUS NUCLEOCAPSID PROTEIN; MITOGENS; PNEUMONIA; SEVERE ACUTE RESPIRATORY SYNDROME (SARS);

VIRUSES;

ACTIN; CASPASE 3; CASPASE 7; FAS ANTIGEN; FIBRONECTIN; FOCAL ADHESION KINASE; GROWTH FACTOR; MITOGEN ACTIVATED PROTEIN KINASE; MITOGEN ACTIVATED PROTEIN KINASE P38; NUCLEOCAPSID PROTEIN; PROTEIN BCL 2; PROTEIN KINASE B; PROTEIN P53; STRESS ACTIVATED PROTEIN KINASE;

ACTIN POLYMERIZATION; ACTIN REORGANIZATION; ANIMAL CELL; APOPTOSIS; ARTICLE; CELL STRAIN COS1; CONTROLLED STUDY; CORONAVIRUS; CYTOSKELETON; DOWN REGULATION; ENZYME ACTIVATION; ENZYME ACTIVITY; GENE SEQUENCE; HUMAN; HUMAN CELL; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; SARS CORONAVIRUS; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SEVERE ACUTE RESPIRATORY SYNDROME; SIGNAL TRANSDUCTION; STRESS; TISSUE INJURY; UPREGULATION; VIRUS GENOME; VIRUS ISOLATION; VIRUS PNEUMONIA;

ACTINS; ANIMALS; APOPTOSIS; CASPASES; CELL LINE, TUMOR; CERCOPITHECUS AETHIOPS; COS CELLS; CULTURE MEDIA, SERUM-FREE; DOWN-REGULATION; FIBRONECTINS; FOCAL ADHESION PROTEIN-TYROSINE KINASES; GROWTH SUBSTANCES; INTEGRINS; MITOGEN-ACTIVATED PROTEIN KINASES; NUCLEOCAPSID PROTEINS; PROTEIN-SERINE-THREONINE KINASES; PROTEIN-TYROSINE KINASES; PROTO-ONCOGENE PROTEINS; PROTO-ONCOGENE PROTEINS C-AKT; SARS VIRUS; TRANSFECTION; UP-REGULATION;

ANIMALIA; CORONAVIRUS; MAMMALIA; RNA VIRUSES; SARS CORONAVIRUS;

EID: 6344233443     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20040984     Document Type: Article

References (13)

1. Drosten, C., Gunther, S., Preiser, W., van der Werf, S., Brodt, H. R., Becker, S., Rabenau, H., Panning, M., Kolesnikova, L., Fouchier, R. A. et al. (2003) Identification of a novel coronavirus in patients with severe acute respiratory syndrome. N. Engl. J. Med. 348, 967-1976
2. Ksiazek, T. G., Erdman, D., Goldsmith, C. S., Zaki, S. R., Peret, I, Emery, S., Tong, S., Urbani, C., Comer, J. A., Lim, W. et al. (2003) A novel coronavirus associated with severe acute respiratory syndrome. N. Engl. J. Med. 348, 1953-1966
3. Rota, P. A., Oberste, M. S., Monroe, S. S., Nix, W. A., Campagnoli, R., Icenogle, J. P., Penaranda, S., Bankamp, B., Maher, K., Chen, M. H. et al. (2003) Characterization of a novei coronavirus associated with severe acute respiratory syndrome. Science 300, 1394-1399
4. Marra, M. A., Jones, S. J., Astell, C. R., Holt, R. A., Brooks-Wilson, A., Butterfield, Y. S., Khattra, J., Asano, J. K., Barber, S. A., Chan, S. V. et al. (2003) The genome sequence of the SARS-associated coronavirus. Science 300, 1399-1404
5. Surjit, M., Liu, B., Kumar, P., Chow, V. T. K. and Lal, S. K. (2004) The nucleocapsid protein of the SARS coronavirus is capable of self-association through a C-terminal 209 amino acid interaction domain. Biochem. Biophys. Res. Commun. 317, 1030-1036
6. He, R., Leeson, A., Andonov, A., Li, Y., Bastien, N., Cao, J., Osiowy, C., Dobie, F., Cutts, T., Ballantine, M. and Li, X. (2003) Activation of AP-1 signal transduction pathway by SARS coronavirus nucleocapsid protein. Biochem. Biophys. Res. Commun. 311, 870-876
7. Yan, H., Xiao, G., Zhang, J., Hu, Y., Yuan, F., Cole, D. K., Zheng, C. and Gao, G. F. (2004) SARS coronavirus induces apoptosis in Vero E6 cells. J. Med. Virol. 73, 323-331
8. Aplin, A. F., Howe, A., Alahari, S. K. and Juliano, R. L. (1998) Signal transduction and signal modulation by cell adhesion receptors: the role of integrins, cadherins, immunoglobulin-cell adhesion molecules, and selectins. Pharmacol. Rev. 5, 197-263
9. Schratt, G., Philippar, U., Berger, J., Schwarz, H., Heidenreich, O. and Nordheim, A. (2002) Serum response factor is crucial for actin cytoskeletal organization and focal adhesion assembly in embryonic stem cells. J. Cell Biol. 156, 737-750
10. Datta, S. R., Brunet, A. and Greenberg, M. E. (1999) Cellular survival: a play in three Akts. Genes Dev. 13, 2905-2927
11. Lang, Z. W., Zhang, L. J., Zhang, S. J., Meng, X., Li, J. Q., Song, C. Z., Sun, L., Znou, Y. S. and Dwyer, D. E. (2003) A clinicopathological study of three cases of severe acute respiratory syndrome (SARS). Pathology 35, 526-531
12. Tse, G. M., To, K. F., Chan, P. K., Lo, A. W., Ng, K. C., Wu, A., Lee, N., Wong, H. C., Mak, S. M., Chan, K. F. et al. (2004) Pulmonary pathological features in coronavirus associated severe acute respiratory syndrome (SARS). J. Clin. Pathol. 57, 260-265
13. Leung, D. T., Tam, F. C., Ma, C. H., Chan, P. K., Cheung, J. L., Niu, H., Tam, J. S. and Lim, P. L. (2004) Antibody response of patients with severe acute respiratory syndrome (SARS) targets the viral nucleocapsid. J. Infect. Dis. 190, 379-386