Development of high-throughput spermidine synthase activity assay using homogeneous time-resolved fluorescence

Analytical Biochemistry

Volumn 351, Issue 2, 2006, Pages 229-240

  Enomoto, Koji ^a   Nagasaki, Tohru ^a   Yamauchi, Akira ^a   Onoda, Junji ^a   Sakai, Katsunori ^a   Yoshida, Tetsuya ^a   Maekawa, Kazuhiko ^a   Kinoshita, Yuko ^a   Nishino, Ikuko ^a   Kikuoka, Shino ^a   Fukunaga, Takahiro ^a   Kawamoto, Keiko ^a   Numata, Yoshito ^a   Takemoto, Hiroshi ^a   Nagata, Kiyoshi ^a  

^a SHIONOGI AND CO LTD   (Japan)

Author keywords

Competitive immunoassay; Homogeneous time resolved fluorescence; Monoclonal antibody; Polyamine; Rare earth cryptate; Spermidine synthase

Indexed keywords

AMINES; CELL PROLIFERATION; ENERGY TRANSFER; EUROPIUM; IMMUNOLOGY; MONOCLONAL ANTIBODIES; RARE EARTHS; SCREENING;

AUTOMATIC SCREENING; COMPETITIVE IMMUNOASSAY; CROSS REACTIVITY; POLYAMINES; PROPYLAMINE GROUPS; S-ADENOSYLMETHIONINE; SYNTHASES; TIME-RESOLVED FLUORESCENCE;

FLUORESCENCE;

ADENOSINE DERIVATIVE; ALLOPHYCOCYANIN; ENZYME INHIBITOR; METHYLTHIOADENOSINE; MONOCLONAL ANTIBODY; POLYAMINE DERIVATIVE; S ADENOSYLMETHIONINE; SPERMIDINE SYNTHASE; UNCLASSIFIED DRUG;

AMINO ACID ANALYSIS; ANTIBODY SPECIFICITY; ARTICLE; CROSS REACTION; DRUG SYNTHESIS; ENERGY TRANSFER; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME MECHANISM; FLUORESCENCE; HIGH THROUGHPUT SCREENING; IMMUNOASSAY; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SCREENING;

EID: 33645296033     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2006.01.012     Document Type: Article

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