Homogeneous immunoassays using rare earth cryptates and time resolved fluorescence : Principles and specific advantages for tumor markers

Anticancer Research

Volumn 17, Issue 4 B, 1997, Pages 3011-3014

  Mathis, Gerard ^a   Socquet, Francoise ^a   Viguier, Mireille ^a   Darbouret, Bruno ^a  

^a CIS Bio International   (France)

Author keywords

AFP; Fluorescence; Homogenous immunoassay; PSA; Tumor markers

Indexed keywords

BIPYRIDINE; LANTHANIDE; LIGAND; PROSTATE SPECIFIC ANTIGEN; TUMOR MARKER;

ANALYTIC METHOD; CONFERENCE PAPER; CONTROLLED STUDY; ENERGY TRANSFER; FLUORESCENCE ANALYSIS; HUMAN; HUMAN TISSUE; IMMUNOASSAY; PRIORITY JOURNAL;

ALPHA-FETOPROTEINS; EUROPIUM; FLUORESCENCE; HUMANS; IMMUNOASSAY; PROSTATE-SPECIFIC ANTIGEN; TUMOR MARKERS, BIOLOGICAL;

EID: 0030865745     PISSN: 02507005     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Conference Paper

References (16)

1. Ullman EF, Bellet NF, Brinkley JM, Zuk RF: Homogeneous fluorescence immunoassays. In: Nakamura RM, Dito WR Tucker ES, eds. Immunoassays: clinical laboratory techniques for the 1980s. New York: AR Liss: 13-43, 1980.
2. Ozinskas AJ, Malak H, Joshi J, Szmacinski H, Britz J, Thompson RB: Homogeneous model immunoassay of thyroxin by phase-modulation spectroscopy. Anal Biochem 213: 264-270, 1993.
3. Mathis G: Rare earth cryptates and homogeneous fluoroimmunoassays with human sera. Clin Chem 39: 1953-1959, 1993.
4. Alpha B, Lehn JM: Energy transfer luminescence of Eu (III) and Tb (III) cryptates of macrobicyclic polypyridine ligands. Angew Chem Int Ed Engl 26: 266-267, 1987.
5. Mathis G, Lehn JM: Macropolycyclic rare earth complexes and application as fluorescent tracers. Patent EP 180492, 1984.
6. Mathis G, Dumont C, Jolu EJP: Method for reducing interferences in a fluorescent assay. Patent WO 92/01224, 1990.
7. Gudgin Dickson EF, Pollack A, Diamandis EP: Time resolved detection of lanthanide luminescence for ultrasensitive bioanalytical assays. J Photochem Photobiol 27: 3-19, 1995
8. Mabile M, Mathis G, Jolu EJP, Pouyat D, Dumont C: Method of measuring luminescence in an assay by luminescence. Patent WO 92/13264, 1991.
9. Seguin P: to be published.
10. Lopez E, Chypre C, Alpha B, Mathis G: Europium (III) trisbipyridine cryptate label for time resolved fluorescence detection of polymerase chain reaction products fixed on solid support. Clin Chem 39: 196-201, 1993.
11. Mathis G, Dumont C, Aspe D, Foyentin M, Jolu EJP, Nuti D: Method for amplifying a signal emitted by a luminescent compound. Patent WO 92/01225, 1990.
12. Kronic MN. J Immun Meth 9: 1-13, 1986.
13. Sadler WA, Smith MH: A computer program for variance function estimation, with particular reference to immunoassay data. Comput-Biomed-Res 20(1): 1-11, 1987
14. Mathis G, Socquet F, Viguier M, Darbouret B, Jolu EJP: Amplified homogeneous time resolved immunofluorometric assay of prolactin. Clin Chem 39: 1251, 1993
15. Mathis G: Probing molecular interactions with homogeneous techniques based on rare earth cryptates and fluorescence energy transfer. Clin Chem 41: 1391-1397, 1995
16. Hoffman KL, Parsons GH, Allerdt LJ, Brooks JM and Miles LEM: Elimination of «Hook Effect» in two-site immunoradiometric assays by kinetic rate analysis. Clin Chem 33(9): 1499-1501, 1984.