Development of a CD28/CD86 (B7-2) binding assay for high throughput screening by homogeneous time-resolved fluorescence

Journal of Biomolecular Screening

Volumn 3, Issue 2, 1998, Pages 91-99

  Mellor, Geoffrey W ^a   Neil Burden, M ^a   Preaudat, Marc ^b   Joseph, Yvonne ^a   Cooksley, Susan B ^a   Ellis, Jonathan H ^a   Banks, Martyn N ^a  

^a GLAXOSMITHKLINE   (United Kingdom)

^b CIS Bio International   (France)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 18844418158     PISSN: 10870571     EISSN: None     Source Type: Journal    
DOI: 10.1177/108705719800300205     Document Type: Article

References (12)

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2. June, C.H., Bluestone, J.A., Nadler, L.M., Thompson, C.B. (1994). The B7 and CD28 receptor families. Immunol. Today 15:321.
3. Linsley, P.S. (1995). Distinct roles for CD28 and cytotoxic T lymphocyte-associated molecule-4 receptors during T cell activation? J. Exp. Med. 182:289.
4. Lenschow, D.J., Walunas, T.L., Bluestone, J.A. (1996). CD28/B7 system of T cell costimulation. Ann. Rev. Immunol. 14:233.
5. Mathis, G. (1995). Probing molecular interactions with homogeneous techniques based on rare earth cryptates and fluorescence energy transfer. Clin. Chem. 41:1391.
6. Kolb, J.M., Yamanaka, G., Manly, S.P. (1996). Use of a novel homogeneous fluorescent technology in high throughput screening. J. Biomolecular Screening 1:203.
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8. van der Merwe, P.A., Bodian, D.L., Daenke, S., Linsley, P., Davis, S.J. (1997). CD80 (B7-1) binds both CD28 and CTLA-4 with a low affinity and very fast kinetics. J. Exp. Med. 185:393.
9. Bebbington, C.R., Renner, G., Thomson, S., King, D., Abrams, D., Yarranton, D.G. (1992). High level expression of a recombinant antibody from myeloma cells using a glutamine synthetase gene as an amplifiable selectable marker. Biotechnology 10:169.
10. Fogarty, S.J., Mellor, G.W., Banks, M.N., Mills, K.M., O'Brien, M.S., Jefferies, B., Houston, J.G. (1996). Automation of scintillation proximity assay (SPA) high throughput screening on the Robolab 9600. Proximity News Issue 29.
11. Mellor, G.W., Fogarty, S.J., O'Brien, M.S., Congrevee, M., Banks, M.N., Mills, K.M., Jefferies, B., Houston, J.G. (1997). Searching for chemokine receptor binding antagonists by high throughput screening. J. Biomolecular Screening 2:153.
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