Characterization of EngF from Clostridium cellulovorans and identification of a novel cellulose binding domain

Applied and Environmental Microbiology

Volumn 64, Issue 3, 1998, Pages 1086-1090

  Ichi Ishi, Akihiko ^a,b   Sheweita, Salah ^a,c   Doi, Roy H ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b TOYO UNIVERSITY   (Japan)

^c ALEXANDRIA UNIVERSITY   (Egypt)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; CELLOBIOSE; CELLULOSE; GLUCAN SYNTHASE; GLUCOSE; MALTOSE;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; CLOSTRIDIUM; ENZYME ACTIVITY; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; THIN LAYER CHROMATOGRAPHY;

EID: 0031884188     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.64.3.1086-1090.1998     Document Type: Article

References (24)

1. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. 1989. Current protocols in molecular biology. John Wiley & Sons, Inc., New York, N.Y.
2. Doi, R. H., M. Goldstein, S. Hashida, J.-S. Park, and M. Takagi. 1994. The Clostridium cellulovorans cellulosome. Crit. Rev. Microbiol. 20:87-93.
3. Foong, F., and R. H. Doi. 1992. Characterization and comparison of Clostridium cellulovorans endoglucanases-xylanases EngB and EngD hyperexpressed in Escherichia coli. J. Bacteriol. 174:1403-1409.
4. Foong, F., T. Hamamoto, O. Shoseyov, and R. H. Doi. 1991. Nucleotide sequence and characteristics of endoglucanase gene engB from Clostridium cellulovorans. J. Gen. Microbiol. 137:1729-1736.
5. Fujino, T., E. Fujino, S. Karita, and K. Ohmiya. 1996. GenBank accession number D85526.
6. Fukumori, F., T. Kudo, and K. Horikoshi. 1987. Truncation analysis of an alkaline cellulase from an alkalophilic Bacillus species. FEMS Microbiol. Lett. 40:311-314.
7. Fukumori, F., T. Kudo, Y. Narahashi, and K. Horikoshi. 1986. Molecular cloning and nucleotide sequence of the alkaline cellulase gene from the alkalophilic Bacillus sp. strain 1139. J. Gen. Microbiol. 132:2329-2335.
8. Fukumori, F., T. Kudo, N. Sashihara, Y. Nagata, K. Ito, and K. Horikoshi. 1989. The third cellulase of alkalophilic Bacillus sp. strain N-4: evolutionary relationships within the cel gene family. Gene 76:289-298.
9. Garcia, E., D. Johnston, J. R. Whitaker, and S. P. Shoemaker. 1993. Assessment of endo-1,4-β-D-glucanase activity by a rapid colorimetric assay using disodium 2,2′-bicinchoninate. J. Food Biochem. 17:135-145.
10. Goldstein, M. A., M. Takagi, S. Hashida, O. Shoseyov, R. H. Doi, and I. H. Segel. 1993. Characterization of the cellulose binding domain of the Clostridium cellulovorans cellulose binding protein A. J. Bacteriol. 175:5762-5768.
11. Hamamoto, T., F. Foong, O. Shoseyov, and R. H. Doi. 1992. Analysis of functional domains of endoglucanases from Clostridium cellulovorans by gene cloning, nucleotide sequencing and chimeric protein construction. Mol. Gen. Genet. 231:472-479.
12. Hamamoto, T., O. Shoseyov, F. Foong, and R. H. Doi. 1990. A Clostridium cellulovorans gene, engD. codes for both endo-β-1,4-glucanase and cellobiosidase activities. FEMS Microbiol. Lett. 72:285-288.
13. Kunkel, T. A. 1985. Rapid and efficient site specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA 82:488-492.
14. Miyatake, M., and K. Imada. 1996. GenBank accession number D85236.
15. Ozaki, K., Y. Hayashi, N. Sumitomo, S. Kawai, and S. Ito. 1995. Construction, purification and properties of a truncated alkaline endoglucanase from Bacillus sp. KSM-635. Biosci. Biotechnol. Biochem. 59:1613-1618.
16. Ozaki, K., S. Shikata, S. Kawai, S. Ito, and K. Okamoto. 1990. Molecular cloning and nucleotide sequence of a gene for alkaline cellulase from Bacillus sp. KSM-635. J. Gen. Microbiol. 136:1327-1334.
17. Sakoda, M., and K. Hiromi. 1976. Determination of best-fit values of kinetic parameters of the Michaelis-Menten equation by the method of least squares with the Taylor expansion. J. Biochem. 80:547-555.
18. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
19. Sheweita, S., A. Ichi-ishi, J.-S. Park, C.-C. Liu, L. M. Malburg, Jr., and R. H. Doi. 1996. Characterization of engF, a gene for a non-cellulosomal Clostridium cellulovorans endoglucanase. Gene 182:163-167.
20. Sumitomo, N., N. Ozaki, S. Kawai, and S. Ito. 1992. Nucleotide sequence of the gene for an alkaline endoglucanase from an alkalophilic Bacillus and its expression in Escherichia coli and Bacillus subtilis. Biosci. Biotechnol. Biochem. 56:872-877.
21. Thompson, J. D., D. G. Higgins, and T. J. Gibson. 1994. Clustal w - improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
22. Tomme, P., R. A. J. Warren, R. C. J. Miller, D. G. Kilburn, and N. R. Gilkes. 1995. Cellulose binding domains: classification and properties, p. 142-161. In J. N. Saddler and M. H. Penner (ed.), Enzymatic degradation of insoluble carbohydrates. American Chemical Society, Washington, D.C.
23. Warren, R. A. J. 1996. Microbial hydrolysis of polysaccharides. Annu. Rev. Microbiol. 50:183-212.
24. Wood, T. M. 1988. Preparation of crystalline, amorphous and dyed cellulase substrates. Methods Enzymol. 160:19-25.