First isolation of Shiga toxin 1d producing Escherichia coli variant strains in shellfish from coastal areas in France

Journal of Applied Microbiology

Volumn 100, Issue 1, 2006, Pages 85-97

  Gourmelon, M ^a   Montet, M P ^b   Lozach, S ^a   Le Mennec, C ^a   Pommepuy, M ^a   Beutin, L ^c   Vernozy Rozand, C ^b  

^a IFREMER   (France)

^b VETAGRO SUP   (France)

^c FEDERAL INSTITUTE FOR RISK ASSESSMENT   (Germany)

Author keywords

Coastal environment; Escherichia coli O157; Shellfish; Shellfish farming areas; Shiga like toxin producing E. coli

Indexed keywords

COASTAL ZONES; ESCHERICHIA COLI; FARMS; GENES; MOLLUSCS; POLYMERASE CHAIN REACTION; VEHICLE TRANSMISSIONS;

COASTAL ENVIRONMENTS; E. COLI; E.COLI O157:H7; ESCHERICHIA COLI O157; SHELLFISH FARMINGS; SHELLFISH-FARMING AREA; SHIGA TOXIN-PRODUCING ESCHERICHIA COLI; SHIGA-LIKE TOXIN-PRODUCING E COLI; SHIGA-LIKE TOXINS; STX GENES;

SHELLFISH;

SHIGA TOXIN 1D; UNCLASSIFIED DRUG; VEROTOXIN 1;

BACTERIUM; COASTAL WATER; IDENTIFICATION METHOD; SHELLFISH;

ANIMAL TISSUE; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM COLONY; BACTERIUM ISOLATION; COASTAL WATERS; CONTROLLED STUDY; DNA HYBRIDIZATION; FRANCE; GENE ISOLATION; IMMUNOMAGNETIC SEPARATION; MOLECULAR PROBE; MUSSEL; NONHUMAN; NUCLEOTIDE SEQUENCE; OYSTER; POLYMERASE CHAIN REACTION; SHELLFISH; SHIGA TOXIN PRODUCING ESCHERICHIA COLI;

EURASIA; EUROPE; FRANCE; WESTERN EUROPE;

ANIMALIA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; OSTREIDAE;

EID: 33645113132     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2005.02753.x     Document Type: Article

References (69)

1. Ackman D. Marks S. Mack P. Caldwell M. Root T. Birkhead G. 1997 Swimming-associated haemorrhagic colitis due to Escherichia coli O157:H7 infection: Evidence of prolonged contamination of a fresh water lake Epidemiol Infect 119 1 8
2. Anon 1991 Council Directive of 15 July 1991 Laying Down the Health Conditions for the Production and the Placing on the Market of Live Bivalve Molluscs (91/492/EEC) Off J Eur Commun no. L268 24.9.91 1 14
3. Anon 1998 ISO 9308-3 Water Quality - Detection and Enumeration of Escherichia coli and Coliform Bacteria in Surface and Waste Water - Part 3: Miniaturized Method (Most Probable Number) by Inoculation in Liquid Medium ISO 20 pp
4. Anon 2000 NF V 08-600 Microbiologie des aliments - Dénombrement des Escherichia coli présumés dans les coquillages vivants - Technique du nombre le plus propable (Microbiologie of Foods and Foodstuffs Products - Enumeration of Presumptive Escherichia coli in Living Shellfish - MPN Technique) AFNOR 16 pp
5. Anon 2001 ISO 16654 Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Escherichia coli O157 ISO 13 pp
6. Anon 2002 NF V 08-106 Dénombrement des Escherichia coli présumés dans les coquillages vivants - Technique indirecte par impédancemétrie directe (Enumeration of Presumptive Escherichia coli in Living Shellfish - Indirect Technique Using Direct Impedance Measurement) AFNOR 16 pp
7. Bastian S.N. Carle I. Grimont F. 1998 Comparaison of 14 PCR systems for the detection and subtyping of stx genes in Shiga toxin-producing Escherichia coli Res Microbiol 149 457 472
8. Beutin L. Geier D. Steinruck H. Zimmermann S. Scheutz F. 1993 Prevalence and some properties of verotoxin (Shiga-like toxin)-producing Escherichia coli in seven different species of healthy domestic animals J Clin Microbiol 31 2483 2488
9. Beutin L. Zimmermann S. Gleier K. 1996 Rapid detection and isolation of shiga-like toxin (verocytotoxin)-producing Escherichia coli by direct testing of individual enterohemolytic colonies from washed sheep blood agar plates in the VTEC-RPLA assay J Clin Microbiol 34 2812 2814
10. Beutin L. Zimmermann S. Gleier K. 1998 Human infections with Shiga toxin-producing Escherichia coli other than serogroup O157 in Germany Emerg Infect Dis 4 635 639
11. Beutin L. Strauch E. Fischer I. 1999 Isolation of Shigella sonnei lysogenic for a bacteriophage encoding gene for production of Shiga toxin Lancet 353 1498
12. Bouvet J. Bavai C. Rossel R. Le Roux A. Montet M.P. Ray Gueniot S. Mazuy C. Arquilliere C. et al. 2001 Prevalence of verotoxin-producing Escherichia coli and E. coli O157: H7 in pig carcasses from three French slaughterhouses Int J Food Microbiol 71 249 255
13. Bouvet J. Montet M.P. Rossel R. Le Roux A. Bavai C. Ray Gueniot S. Mazuy C. Atrache V. et al. 2002 Prevalence of verotoxin-producing Escherichia coli (VTEC) and E. coli O157: H7 in French pork J Appl Microbiol 93 7 14
14. Bürk C. Dietrich R. Açar G. Moravek M. Bülte M. Märtlbauer E. 2003 Identification and characterization of a new variant of Shiga toxin 1 in Escherichia coli ONT:H19 of bovine origin J Clin Microbiol 41 2106 2112
15. Cebula T. Payne W. Feng P. 1995 Simultaneous identification of strains of Escherichia coli serotype O157:H7 and their Shiga-like toxin type by mismatch amplification mutation assay-multiplex PCR J Clin Microbiol 33 248 250
16. Chapman P.A. Siddons C.A. Gerdan Malo A.T. Harkin M. 1997 A 1-year study of Escherichia coli O157 in cattle, sheep, pigs and poultry Epidemiol Infect 119 245 250
17. Chapman P.A. Ellin M. Ashton R. Shafique W. 2001 Comparison of culture, PCR and immunoassays for detecting Escherichia coli O157 following enrichment culture and immunomagnetic separation performed on naturally contaminated raw meat products Int J Food Microbiol 68 11 20
18. Conedera G. Dalvit P. Martini M. Galiero G. Gramaglia M. Goffredo E. Loffredo G. Morabito S. et al. 2004 Verocytoxin-producing Escherichia coli O157 in minced beef and diary products in Italy Int J Food Microbiol 96 67 73
19. Duffy G. 2003 Verocytoxigenic Escherichia coli in animal faeces, manures and slurries J Appl Microbiol 94 94S 103S
20. Dupont J. Dumont F. Menanteau C. Pommepuy M. 2004 Calibration of the impedance method for rapid quantitative estimation of Escherichia coli in live marine bivalve molluscs J Appl Microbiol 96 894 902
21. Dupray E. Caprais M.P. Derrien A. Monfort P. Convenant A. Penot J. Fach P. Dilasser F. et al. 1999 Flux bactériens et qualité sanitaire des coquillages en baie de la Fresnaye In Pollutions diffuses: du bassin versant au littoral ed Merceron M. pp 169 178 Plouzané Ifremer
22. Fagan P.K. Hornitzky M.A. Bettelheim K.A. Djordjevic S.P. 1999 Detection of shiga-like toxin (stx1 and stx2), intimin (eaeA), and enterohemorrhagic Escherichia coli (EHEC) hemolysin (EHEC hlyA) genes in animal feces by multiplex PCR Appl Environ Microbiol 65 5177 5181
23. Feldhusen F. 2000 The role of seafood in bacterial foodborne diseases Microbes Infect 2 1651 1660
24. Griffin P.M. Tauxe R.V. 1991 The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic E. coli, and the associated hemolytic uremic syndrome Epidemiol Rev 13 60 98
25. Guyon R. Dorey F. Collobert J.F. Foret J. Goubert C. Mariau V. Malas J.P. 2000 Detection of Shiga toxin-producing Escherichia coli O157 in shellfish (Crassostrea gigas) Sci Aliments 20 457 465
26. Hancock D. Besser T. Le Jeune J. Davis M. Rice D. 2001 The control of VTEC in the animal reservoir Int J Food Microbiol 66 71 78
27. Hervio-Heath D. Colwell R.R. Derrien A. Robert-Pillot A. Fournier J.M. Pommepuy M. 2002 Occurrence of pathogenic vibrios in coastal areas of France J Appl Microbiol 92 1123 1135
28. Höller C. Koschinsky S. Witthuhn D. 1999 Isolation of enterohaemorrhagic Escherichia coli from municipal sewage Lancet 353 2039
29. Ibekwe A.M. Watt P.M. Grieve C.M. Sharma V.K. Lyons S.R. 2002 Multiplex fluorogenic real-time PCR for detection and quantification of Escherichia coli O157:H7 in dairy wastewater wetlands Appl Environ Microbiol 68 4853 4862
30. Johnson W.M. Pollard D.R. Lior H. Tyler S.D. Rozee K.R. 1990 Differentiation of genes coding for Escherichia coli verotoxin 2 and the verotoxin associated with porcine edema disease (VTe) by the polymerase chain reaction J Appl Microbiol 28 2351 2353
31. Karch H. Meyer T. Rüssmann H. Heesemann J. 1992 Frequent loss of Shiga-like toxin genes in clinical isolates of Escherichia coli upon subcultivation Infect Immun 60 3464 3467
32. Kumar H.S. Otta S. Karunasagar I. 2001 Detection of Shiga-toxigenic Escherichia coli (STEC) in fresh seafood and meat marketed in Mangalore, India by PCR Lett Appl Microbiol 33 334 338
33. Kumar H.S. Karunasagar I. Karunasagar I. Teizou T. Shima K. Yamasaki S. 2004 Characterisation of Shiga toxin-producing Escherichia coli (STEC) isolated from seafood and beef FEMS Microbiol Lett 233 173 178
34. Le Guyader F. Haugarreau L. Miossec L. Dubois E. Pommepuy M. 2000 Three-year study to assess human enteric viruses in shellfish Appl Environ Microbiol 66 3241 3248
35. Lee R.J. Morgan O.C. 2003 Environmental factors influencing the microbiological contamination of commercially harvested shellfish Water Sci Technol 47 65 70
36. Lipp E.K. Rose J.B. 1997 The role of seafood in foodborne diseases in the United States of America Rev Sci Tech 16 620 640
37. Machado J. Grimont F. Grimont P.A.D. 2000 Identification of Escherichia coli flagellar types by restrition of the amplified fliC gene Res Microbiol 151 535 546
38. MacRae M. Strachan N.J.C. Jacklin M. Ogden I.D. 2003 Methods to isolate and incidence of Escherichia coli O157 in UK Shellfish In 5th International Symposium on 'Shiga toxin-producing E. coli infections' VTEC 2003, Edinburgh, 8 to 11 June 2003 (poster presentation).
39. Mead P.S. Griffin P.M. 1998 Escherichia coli O157:H7 Lancet 352 1207 1212
40. Muniesa M. Jofre J. 1998 Abundance in sewage of bacteriophages that infect Escherichia coli O157:H7 and that carry the Shiga toxin 2 gene Appl Environ Microbiol 64 2443 2448
41. Muniesa M. Jofre J. 2000 Occurrence of phages infecting Escherichia coli O157:H7 carrying the Stx 2 gene in sewage from different countries FEMS Microbiol Lett 183 197 200
42. Nakao H. Kimura K. Murakami H. Maruyama T. Tadeka T. 2002 Subtyping of Shiga toxin 2 variants in human-derived Shiga toxin-producing Escherichia coli strains isolated in Japan FEMS Immunol Med Microbiol 34 289 297
43. Nielsen E.M. Andersen M.T. 2003 Detection and characterization of Verocytotoxin-producing Escherichia coli by automated 5′ nuclease PCR assay J Clin Microbiol 41 2884 2893
44. O'Brien A.D.J. Newland J.W. Miller S.F. Holmes R.K. Smith H.W. Formal S.B. 1984 Shiga-like toxin converting phages from E. coli strains that cause hemorrhagic colitis or infantile diarrhea Science 226 694 696
45. Ohmura-Hoshino M. Ho S.T. Kurazono H. Igarashi K. Yamasaki S. Tadeka Y. 2003 Genetic and immunological analysis of a novel variant of Shiga toxin 1 from bovine Escherichia coli strains and development of bead-ELISA to detect the variant toxin Microbiol Immunol 47 717 725
46. Olsen S.J. Miller G. Breuer T. Kennedy M. Higgins C. Walford J. McKee G. Fox K. et al. 2002 A waterborne outbreak of Escherichia coli O157:H7 infections and hemolytic uremic syndrome: implications for rural water systems Emerg Infect Dis 8 370 375
47. Orskov F. Orskov I. 1984 Serotyping of Escherichia coli J Microbiol Methods 14 43 112
48. Paton J.C. Paton A.W. 1997 Instability of a Shiga toxin type 2 gene in Enterobacter cloacae J Clin Microbiol 35 1917
49. Paton J.C. Paton A.W. 1998a Pathogenesis and diagnosis of Shiga toxin-producing Escherichia coli infections Clin Microbiol Rev 11 450 479
50. Paton A.W. Paton J.C. 1998b Detection and characterization of shiga toxigenic Escherichia coli by using multiplex PCR assays for stx(1), stx(2), eaeA, enterohemorrhagic E-colihlyA, rfb(O111), and rfb (O157) J Clin Microbiol 36 598 602
51. Potasman I. Paz A. Odeh M. 2002 Infectious outbreaks associated with bivalve shellfish consumption: a worldwide perspective Clin Infect Dis 35 921 928
52. Rampersad F.S. Laloo S. La Borde A. Maharaj K. Sookhai L. Teelucksingh J. Reid S. McDougall L. et al. 1999 Microbial quality of oysters sold in western Trinidad and potential health risk to consumers Epidemiol Infect 123 241 250
53. Read S.C. Clarke R.C. Martin A. De Grandis S.A. Hii J. McEwen S. Gyles C.L. 1992 Polymerase chain reaction for detection of verocytotoxigenic Escherichia coli isolated from animal and food sources Mol Cell Probes 6 153 161
54. Reid S.D. Betting D.J. Whitlam T.S. 1999 Molecular detection and identification of intimin alleles in pathogenic Escherichia coli by multiplex PCR J Clin Microbiol 37 2719 2722
55. Riley L.W. Remis R.S. Helgerson S.D. Mac Gee H.B. Wells J.G. Davis B.R. Herbert R.J. Johson L.M. et al. 1983 Hemorrhagic colitis associated with a rare Escherichia coli serotype N Engl J Med 308 681 685
56. Rodrigues J. Scaletsky I.C.A. Campos L.C. Gomes T.A.T. Whittam T.S. Trabulsi L.R. 1996 Clonal structure and virulence factors in strains of Escherichia coli of the class serogroup O55 Infect Immun 64 2680 2686
57. Rozen Y. Belkin S. 2001 Survival of enteric bacteria in seawater FEMS Microbiol Rev 25 513 529
58. Samadpour M. Ongerth J.E. Liston J. Tran N. Nguyen D. Whittam T.S. Wilson R.A. Tarr P.I. 1994 Occurence of shiga-like toxin-producing Escherichia coli in retail fresh seafood, beef, lamb, pork, and poultry from grocery stores in Seattle, Washington App Environ Microbiol 60 1038 1040
59. Schmidt H. 2001 Shiga-toxin-converting bacteriophages Res Microbiol 152 687 695
60. Schmidt H. Montag M. Bockemühl J. Heesemann J. Karch H. 1993 Shiga-like toxin II-related cytotoxins in Citrobacter freundii strains from humans and beef samples Infect Immun 61 534 543
61. Schmidt H. Scheef J. Huppertz H.I. Frosch M. Karch H. 1999 Escherichia coli O157:H7 and O157:H- strains that do not produce Shiga toxin: phenotypic and genetic characterization of isolates associated with diarrhea and haemolytic-uremic syndrome J Clin Microbiol 37 3491 3496
62. Sharma V.K. Dean-Nystrom E.A. Casey T.A. 1999 Semi-automated fluorogenic PCR assays (TaqMan) forrapid detection of Escherichia coli O157:H7 and other Shiga toxigenic E. coli Mol Cell Probes 13 291 302
63. Troussellier M. Bonnefont J.-L. Courties C. Derrien A. Dupray E. Gauthier M. Gourmelon M. Joux F. et al. 1998 Responses of enteric bacteria to environmental stresses in seawater Oceanol Acta 21 965 981
64. Vernozy-Rozand C. Feng P. Montet M.P. Ray-Gueniot S. Villard L. Bavai C. Meyrand A. Mazuy C. et al. 2000 Detection of Escherichia coli O157:H7 in heifers' faecal samples using an automated immunoconcentration system Lett Appl Microbiol 30 217 222
65. Vernozy-Rozand C. Montet M. Lequerrec F. Serillon E. Tilly B. Bavai C. Ray-Gueniot S. Bouvet J. et al. 2002 Prevalence of verotoxin-producing Escherichia coli (VTEC) in slurry, farmyard manure and sewage sludge in France J Appl Microbiol 93 473 478
66. Vernozy-Rozand C. Montet M.P. Bertin Y. Trably F. Girardeau J.P. Martin C. Livrelli V. Beutin L. 2004 Serotyping, stx2 subtyping, and characterisation of the locus of enterocyte effacement island of Shiga-toxin-producing Escherichia coli and E. coli O157:H7 strains isolated from the environment in France Appl Environ Microbiol 70 2556 2559
67. Wallace B.J. Guzewich J.J. Cambridge M. Altekruse S. Morse D.L. 1999 Seafood-associated disease outbreaks in New York, 1980-1994 Am J Prev Med 17 48 54
68. Zhang W. Bielaszewska M. Kuczius T. Karch H. 2002 Identification, characterisation, and distribution of a Shiga toxin 1 gene variant (stx1c) in Escherichia coli strains isolated from humans J Clin Microbiol 40 1441 1446
69. Zschock M. Hamann H.P. Kloppert B. Wolter W. 2000 Shiga-toxin-producing Escherichia coli in faeces of healthy dairy cows, sheep and goats: prevalence and virulence properties Lett Appl Microbiol 31 203 208