Probing gene expression in live cells, one protein molecule at a time

Science

Volumn 311, Issue 5767, 2006, Pages 1600-1603

  Yu, Ji ^a   Xiao, Jie ^a   Ren, Xiaojia ^a   Lao, Kaiqin ^b   Xie, X Sunney ^a  

^a HARVARD UNIVERSITY   (United States)

^b APPLIED BIOSYSTEMS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; ESCHERICHIA COLI; FLUORESCENCE; MOLECULES; PROTEINS; RNA;

FAST-MATURING YELLOW FLUORESCENT PROTEIN (YFP); GEOMETRIC DISTRIBUTION; MEMBRANE-TARGETING PEPTIDE;

GENES;

BACTERIAL PROTEIN; HYBRID PROTEIN; MESSENGER RNA; PEPTIDE; YELLOW FLUORESCENT PROTEIN;

PROTEIN;

ARTICLE; BACTERIAL CELL; BACTERIAL MEMBRANE; ESCHERICHIA COLI; GENE EXPRESSION; GENE PROBE; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; RNA TRANSCRIPTION;

BACTERIAL PROTEINS; BLOTTING, WESTERN; ESCHERICHIA COLI K12; ESCHERICHIA COLI PROTEINS; FLUORESCENCE; GENE EXPRESSION; GENE EXPRESSION PROFILING; LUMINESCENT PROTEINS; MEMBRANE PROTEINS; MICROSCOPY, FLUORESCENCE; MOLECULAR PROBE TECHNIQUES; POISSON DISTRIBUTION; PROMOTER REGIONS (GENETICS); RECOMBINANT FUSION PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, BACTERIAL; RNA, MESSENGER; STOCHASTIC PROCESSES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 33645033645     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1119623     Document Type: Article

References (36)

1. F. Jacob, J. Monod, Cold Spring Harbor Symp. Quant. Biol. 26, 193 (1961).
2. J. Beckwith, Science 156, 597 (1967).
3. W. Gilbert, B. Muller-Hill, Proc. Natl. Acad. Sci. U.S.A. 58, 2415 (1967).
4. M. Ptashne, A. Gann, Genes and Signals (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 2002).
5. X. S. Xie, H. P. Lu, J. Biol. Chem. 274, 15967 (1999).
6. C. Bustamante, Z. Bryant, S. Smith, Nature 421, 423 (2003).
7. A. Ishijima, T. Yanagida, Trends Biochem. Sci. 26, 438 (2001).
8. S. Weiss, Science 283, 1676 (1999).
9. M. D. Wang et al., Science 282, 902 (1998).
10. S. Blanchard, R. Gonzalez, H. Kim, S. Chu, J. Puglisi, Nat. Struct. Mol. Biol. 11, 1008 (2004).
11. O. Berg, J. Theor. Biol. 71, 587 (1978).
12. D. Rigney, J. Theor. Biol. 79, 247 (1979).
13. J. Paulsson, Nature 427, 415 (2004).
14. J. Paulsson, Phys. Life Rev. 2, 157 (2005).
15. Y. Shav-Tal et al., Science 304, 1797 (2004).
16. I. Golding, E. Cox, Proc. Natl. Acad. Sci. U.S.A. 101, 11310 (2004).
17. N. Barkai, S. Leibler, Nature 403, 267 (2000).
18. E. M. Ozbudak, M. Thattai, I. Kurtser, A. D. Grossman, A. van Oudenaarden, Nat. Genet. 31, 69 (2002).
19. M. B. Elowitz, A. J. Levine, E. D. Siggia, P. S. Swain, Science 297, 1183 (2002).
20. N. Rosenfeld, J. W. Young, U. Alon, P. S. Swain, M. B. Elowitz, Science 307, 1962 (2005).
21. S. Ghaemmaghami et al., Nature 425, 737 (2003).
22. P. Guptasarma, Bioessays 17, 987 (1995).
23. Materials and methods are available as supporting material on Science Online.
24. R. J. Bongaerts, I. Hautefort, J. M. Sidebotham, J. C. Hinton, Methods Enzymol. 358, 43 (2002).
25. R. Y. Tsien, Annu. Rev. Biochem. 67, 509 (1998).
26. M. Chalfie, Y. Tu, G. Euskirchen, W. W. Ward, D. C. Prasher, Science 263, 802 (1994).
27. T. Nagai et al., Nat. Biotechnol., 20, 87 (2002).
28. P. H. Lommerse et al., Biophys. J. 86, 609 (2004).
29. J. Deich, E. M. Judd, H. H. McAdams, W. E. Moerner, Proc. Natl. Acad. Sci. U.S.A. 101, 15921 (2004).
30. S. L. Mowbray, M. O. J. Sandgren, J. Struct. Biol. 124, 257 (1998).
31. K. Bondeson, A. Frostellkarlsson, L. Fagerstam, G. Magnusson, Anal. Biochem. 214, 245 (1993).
32. W. Hsieh, P. Whitson, K. Matthews, R. Wells, J. Biol. Chem. 262, 14583 (1987).
33. O. Yarchuk, N. Jacques, J. Guillerez, M. Dreyfus, J. Mol. Biol. 226, 581 (1992).
34. H. H. McAdams, A. Arkin, Proc. Natl. Acad. Sci. U.S.A. 94, 814 (1997).
35. L. Cai, N. Friedman, X. S. Xie, Nature 440, 358 (2006).
36. We thank P. Choi, L. Cai, N. Friedman, J. Elf, L. Xun, and R. Losick for helpful discussions and critical reading of the manuscript, J. Hearn for technical assistance, and A. Miyawaki (RIKEN) for the generous gift of the venus gene. This work was supported by NIH Director's Pioneer Award program, a NIH R21 grant, and in part by U.S. Department of Energy, Office of Science, and Applied Biosystems' Exploratory Fund. J.Y. acknowledges a Genome-Related Research Award from Merck.