Effects of orally administered Lactobacillus casei DN-114001 on the composition or activities of the dominant faecal microbiota in healthy humans

British Journal of Nutrition

Volumn 95, Issue 2, 2006, Pages 421-429

  Rochet, Violaine ^a   Rigottier Gois, Lionel ^a   Sutren, Malène ^a   Krementscki, Marie Noëlle ^a   Andrieux, Claude ^a   Furet, Jean Pierre ^a   Tailliez, Patrick ^a   Levenez, Florence ^a   Mogenet, Agnès ^b   Bresson, Jean Louis ^b   Méance, Séverine ^c   Cayuela, Chantal ^c   Leplingard, Antony ^c   Doré, Joël ^a  

^a CEMAGREF   (France)

^b HÔPITAL NECKER ENFANTS MALADES   (France)

^c DANONE RESEARCH   (France)

Author keywords

Fluorescent in situ hybridisation; Human faecal microbiota; Lactobacillus casei; Probiotic; Real time PCR

Indexed keywords

BACTERIAL DNA; BACTERIAL ENZYME; GENOMIC DNA; YOGHURT; PROBIOTIC AGENT;

ADULT; ARTICLE; BACTERIAL STRAIN; COLONY FORMING UNIT; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DIET SUPPLEMENTATION; DNA EXTRACTION; ENZYME ACTIVITY; FECES ANALYSIS; FECES MICROFLORA; FEMALE; FERMENTATION; FLOW CYTOMETRY; FLUORESCENCE IN SITU HYBRIDIZATION; FOOD INTAKE; GEL ELECTROPHORESIS; GENE SEQUENCE; HUMAN; HUMAN EXPERIMENT; LACTOBACILLUS CASEI; MALE; MILK; NONHUMAN; NORMAL HUMAN; NUTRITIONAL HEALTH; REAL TIME POLYMERASE CHAIN REACTION; CHEMISTRY; DAIRY PRODUCT; ENZYMOLOGY; FECES; ISOLATION AND PURIFICATION; MICROBIOLOGY; ORAL DRUG ADMINISTRATION;

BACTERIA (MICROORGANISMS); LACTOBACILLUS CASEI;

ADMINISTRATION, ORAL; ADULT; CULTURED MILK PRODUCTS; DNA, BACTERIAL; FECES; FEMALE; HUMANS; LACTOBACILLUS CASEI; MALE; PROBIOTICS;

EID: 33644913136     PISSN: 00071145     EISSN: 14752662     Source Type: Journal    
DOI: 10.1079/BJN20051625     Document Type: Article

References (48)

1. Agence Française de Securité Sanitaive des Aliments (2003). Alimentation infantile et modification de la flore intestinale. http://www.afssa.fr/ftp/afssa/basedoc/Floreintestinale.pdf.
2. Alander M, Satokari R, Korpela R, Saxelin M, Vilpponen-Salmela T, Mattila-Sandholm T & van Wright A (1999) Persistence of colonization of human colonic mucosa by a probiotic strain, Lactobacillus rhamnosus GG, after oral consumption. Appl Environ Microbiol 65, 351-354.
3. Amann RI, Binder BJ, Olson RJ, Chisholm SW, Devereux R & Stahl DA (1990) Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl Environ Microbiol 56, 1919-1925.
4. Andrieux C, Membre JM, Cayuela C & Antoine JM (2002) Metabolic characteristics of the faecal microflora in humans from three age groups. Scand J Gastroenterol 37, 792-798.
5. Boehler N, Riottot M, Ferezou J, Souidi M, Milliat F, Serougne C, Smith JL & Lutton C (1999) Antilithiasic effect of beta-cyclodextrin in LPN hamster: comparison with cholestyramine. J Lipid Res 40, 726-734.
6. Bouhnik Y, Flourie B, Riottot M, Bisetti N, Gailing MF, Guibert A, Bornet F & Rambaud JC (1996) Effects of fructo-oligosaccharides ingestion on fecal bifidobacteria and selected metabolic indexes of colon carcinogenesis in healthy humans. Nutr Cancer 26, 21-29.
7. Collins JK, Dunne C, Murphy L, et al. (2002) A randomised controlled trial of a probiotic Lactobacillus strain in healthy adults: assessment of its delivery, transit and influence on microbial flora and enteric immunity. Microbial Ecol Health Dis 14, 81-89.
8. Drouault S & Corthier G (2001) [Health effects of lactic acid bacteria ingested in fermented milk.]. Vet Res 32, 101-117.
9. Franks AH, Harmsen HJ, Raangs GC, Jansen GJ, Schut F & Welling GW (1998) Variations of bacterial populations in human feces measured by fluorescent in situ hybridization with group-specific 16S rRNA-targeted oligonucleotide probes. Appl Environ Microbiol 64, 3336-3345.
10. Fujiwara S, Seto Y, Kimura A & Hashiba H (2001) Intestinal transit of an orally administered streptomycin-rifampicin-resistant variant of Bifidobacterium longum SBT2928: its long-term survival and effect on the intestinal microflora and metabolism. J Appl Microbiol 90, 43-52.
11. Fuller R (1989) Probiotics in man and animals. J Appl Bacteriol 66, 365-378.
12. Furet JP, Quenee P & Tailliez P (2004) Molecular quantification of lactic acid bacteria in fermented milk products using real-time quantitative PCR. Int J Food Microbiol 97, 197-207.
13. Gibson GR, Beatty ER, Wang X & Cummings JH (1995) Selective stimulation of bifidobacteria in the human colon by oligofructose and inulin. Gastroenterology 108, 975-982.
14. Gibson GR, Rastall RA & Roberfroid MB (1999) Prebiotics. In Colonic Microbiota, Nutrition and Health, pp. 101-124 [MB Roberfroid, editor]. Dordrecht: Kluwer Academic.
15. Goldin BR (1998) Health benefits of probiotics. Br J Nutr 80, S203-S207.
16. Goldin BR, Gorbach SL, Saxelin M, Barakat S, Gualtieri L & Salminen S (1992) Survival of Lactobacillus species (strain GG) in human gastrointestinal tract. Dig Dis Sci 37, 121-128.
17. Guérin-Danan C, Chabanet C, Pedone C, Popot F, Vaissade P, Bouley C, Szylit O & Andrieux C (1998) Milk fermented with yogurt cultures and Lactobacillus casei compared with yogurt and gelled milk: influence on intestinal microflora in healthy infants. Am J Clin Nutr 67, 111-117.
18. Harmsen HJ, Elfferich P, Schut F & Welling GW (1999) A 16S rRNA-targeted probe for detection of Lactobacilli and Enterococci in faecal samples by fluorescent In Situ hybridization. Microbial Ecol Health Dis 11, 3-12.
19. Harmsen HJM, Wildeboer-Veloo ACM, Grijpstra J, Knol J, Degener JE & Welling GW (2000) Development of 16S rRNA-based probes for the Coriobacterium group and the Atopobium cluster and their application for enumeration of Coriobacteriaceae in human feces from volunteers of different age groups. Appl Environ Microbiol 66, 4523-4527.
20. Langendijk PS, Schut F, Jansen GJ, Raangs GC, Kamphuis GR, Wilkinson MH & Welling GW (1995) Quantitative fluorescence in situ hybridization of Bifidobacterium spp. with genus-specific 16S rRNA-targeted probes and its application in fecal samples. Appl Environ Microbiol 61, 3069-3075.
21. Lee YK, Ho PS, Low CS, Arvilommi H & Salminen S (2004) Permanent colonization by Lactobacillus casei is hindered by the low rate of cell division in mouse gut. Appl Environ Microbiol 70, 670-674.
22. Lowry OH, Rosebrough NJ, Farr AL & Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193, 265-275.
23. Manz W, Amann R, Ludwig W, Vancanneyt M & Schleifer KH (1996) Application of a suite of 16S rRNA-specific oligonucleotide probes designed to investigate bacteria of the phylum cytophagaflavobacter-bacteroides in the natural environment. Microbiology 142, 1097-1106.
24. Marteau PR, de Vrese M, Cellier CJ & Schrezenmeir J (2001) Protection from gastrointestinal diseases with the use of probiotics. Am J Clin Nutr 73, 430S-436S.
25. Marteau P, Flourie B, Pochart P, Chastang C, Desjeux JF & Rambaud JC (1990) Effect of the microbial lactase (EC 3.2.1.23) activity in yoghurt on the intestinal absorption of lactose: an in vivo study in lactase-deficient humans. Br J Nutr 64, 71-79.
26. Matsuki T, Watanabe K, Fujimoto J, Miyamoto Y, Takada T, Matsumoto K, Oyaizu H & Tanaka R (2002) Development of 16S rRNA-gene-targeted group-specific primers for the detection and identification of predominant bacteria in human feces. Appl Environ Microbiol 68, 5445-5451.
27. Ott SJ, Musfeldt M, Wenderoth DF, Hampe J, Brant O, Folsch UR, Timmis KN & Schreiber S (2004) Reduction in diversity of the colonic mucosa associated bacterial microflora in patients with active inflammatory bowel disease. Gut 53, 685-693.
28. Panda SK, Chattoraj SC & Broitman SA (1999) Correlation of neomycin, faecal neutral and acid sterols with colon carcinogenesis in rats. Br J Cancer 80, 1132-1136.
29. Pedone CA, Arnaud CC, Postaire ER, Bouley CF & Reinert P (2000) Multicentric study of the effect of milk fermented by Lactobacillus casei on the incidence of diarrhoea. Int J Clin Pract 54, 568-571.
30. Pedone CA, Bernabeu AO, Postaire ER, Bouley CF & Reinert P (1999) The effect of supplementation with milk fermented by Lactobacillus casei (strain DN-114 001) on acute diarrhoea in children attending day care centres. Int J Clin Pract 53, 179-184.
31. Rachmilewitz D, Katakura K, Karmeli F, Hayashi T, Reinus C, Rudensky B, Akira S, Takeda K, Lee J, Takabayashi K & Raz E (2004) Toll-like receptor 9 signaling mediates the anti-inflammatory effects of probiotics in murine experimental colitis. Gastroenterology 126, 520-528.
32. Rigottier-Gois L, Le Bourhis A-G, Gramet G, Rochet V & Dore J (2003) Fluorescent hybridisation combined with flow cytometry and hybridisation of total RNA to analyse the composition of microbial communities in human faeces using 16S rRNA probes. FEMS Microbiol Ecol 43, 237-245.
33. Rochet V, Rigottier-Gois L, Beguet F & Doré J (2001) Composition of human intestinal flora analysed by fluorescent in situ hybridisation using group-specific 16S rRNA-targeted oligonucleotide probes. Genet Sel Evol 33, Suppl. 1, S339-S352.
34. Salminen S, Bouley C, Boutron-Ruault MC, Cummings JH, Franck A, Gibson GR, Isolauri E, Moreau MC, Roberfroid M & Rowland I (1998) Functional food science and gastrointestinal physiology and function. Br J Nutr 80, Suppl. 1, S147-S171.
35. Satokari RM, Vaughan EE, Akkermans AD, Saarela M & de Vos WM (2001) Bifidobacterial diversity in human feces detected by genus-specific PCR and denaturing gradient gel electrophoresis. Appl Environ Microbiol 67, 504-513.
36. Seksik P, Rigottier-Gois L, Gramet G, Sutren M, Pochart P, Marteau P, Jian R & Dore J (2003) Alterations of the dominant faecal bacterial groups in patients with Crohn's disease of the colon. Gut 52, 237-242.
37. Sghir A, Gramet G, Suau A, Rochet V, Pochart P & Dore J (2000) Quantification of bacterial groups within human fecal flora by oligonucleotide probe hybridization. Appl Environ Microbiol 66, 2263-2266.
38. Siitonen S, Vapaatalo H, Salminen S, Gordin A, Saxelin M, Wikberg R & Kirkkola AL (1990) Effect of Lactobacillus GG yoghurt in prevention of antibiotic associated diarrhoea. Ann Med 22, 57-59.
39. Suau A, Bonnet R, Sutren M, Godon JJ, Gibson GR, Collins MD & Doré J (1999) Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut. Appl Environ Microbiol 65, 4799-4807.
40. Suau A, Rochet V, Sghir A, Gramet G, Brewaeys S, Sutren M, Rigottier-Gois L & Dore J (2001) Fusobacterium prausnitzii and related species represent a dominant group within the human fecal flora. Syst Appl Microbiol 24, 139-145.
41. Tannock GW (2002) Analysis of the intestinal microflora using molecular methods. Eur J Clin Nutr 56, Suppl. 4, S44-S49.
42. Tannock GW, Munro K, Harmsen HJ, Welling GW, Smart J & Gopal PK (2000) Analysis of the fecal microflora of human subjects consuming a probiotic product containing Lactobacillus rhamnosus DR20. Appl Environ Microbiol 66, 2578-2588.
43. Vaughan EE, Mollet B & deVos WM (1999) Functionality of probiotics and intestinal lactobacilli: light in the intestinal tract tunnel. Curr Opin Biotechnol 10, 505-510.
44. Wallner G, Amann R & Beisker W (1993) Optimizing fluorescent in situ hybridization with rRNA-targeted oligonucleotide probes for flow cytometric identification of microorganisms. Cytometry 14, 136-143.
45. Walter J, Tannock GW, Tilsala-Timisjarvi A, Rodtong S, Loach DM, Munro K & Alatossava T (2000) Detection and identification of gastrointestinal Lactobacillus species by using denaturing gradient gel electrophoresis and species-specific PCR primers. Appl Environ Microbiol 66, 297-303.
46. Wang RF, Cao WW & Cerniglia CE (1996) PCR detection and quantitation of predominant anaerobic bacteria in human and animal fecal samples. Appl Environ Microbiol 62, 1242-1247.
47. Zoetendal EG, Akkermans AD, Akkermans-van Vliet WM, de Visser AGM & De Vos WM (2001) The host genotype affects the bacterial community in the human gastrointestinal tract. Microbial Ecol Health Dis 13, 129-134.
48. Zoetendal EG, Akkermans AD & De Vos WM (1998) Temperature gradient gel electrophoresis analysis of 16S rRNA from human fecal samples reveals stable and host-specific communities of active bacteria. Appl Environ Microbiol 64, 3854-3859.