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Volumn 43, Issue 2, 2003, Pages 237-245

Fluorescent hybridisation combined with flow cytometry and hybridisation of total RNA to analyse the composition of microbial communities in human faeces using 16S rRNA probes

Author keywords

Dot blot hybridisation; Flow cytometry; Fluorescent in situ hybridisation; Human fecal microbiota

Indexed keywords

RIBOSOME RNA; RNA;

EID: 0037445321     PISSN: 01686496     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-6496(02)00416-6     Document Type: Article
Times cited : (131)

References (30)
  • 1
    • 0031768678 scopus 로고    scopus 로고
    • Creating and maintaining the gastrointestinal ecosystem: What we know and need to know from gnotobiology
    • Falk P.G., Hooper L.V., Midtvedt T., Gordon J.I. Creating and maintaining the gastrointestinal ecosystem: what we know and need to know from gnotobiology. Microbiol. Mol. Biol. Rev. 62:1998;1157-1170.
    • (1998) Microbiol. Mol. Biol. Rev. , vol.62 , pp. 1157-1170
    • Falk, P.G.1    Hooper, L.V.2    Midtvedt, T.3    Gordon, J.I.4
  • 4
    • 0016167941 scopus 로고
    • Human fecal flora: The normal flora of 20 Japanese-Hawaiians
    • Moore W.E., Holdeman L.V. Human fecal flora: the normal flora of 20 Japanese-Hawaiians. Appl. Microbiol. 27:1974;961-979.
    • (1974) Appl. Microbiol. , vol.27 , pp. 961-979
    • Moore, W.E.1    Holdeman, L.V.2
  • 5
    • 0032755259 scopus 로고    scopus 로고
    • Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut
    • Suau A., Bonnet R., Sutren M., Godon J.J., Gibson G.R., Collins M.D., Doré J. Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut. Appl. Environ. Microbiol. 65:1999;4799-4807.
    • (1999) Appl. Environ. Microbiol. , vol.65 , pp. 4799-4807
    • Suau, A.1    Bonnet, R.2    Sutren, M.3    Godon, J.J.4    Gibson, G.R.5    Collins, M.D.6    Doré, J.7
  • 6
    • 0029900986 scopus 로고    scopus 로고
    • Human colonic biota studied by ribosomal DNA sequence analysis
    • Wilson K.H., Blitchington R.B. Human colonic biota studied by ribosomal DNA sequence analysis. Appl. Environ. Microbiol. 62:1996;2273-2278.
    • (1996) Appl. Environ. Microbiol. , vol.62 , pp. 2273-2278
    • Wilson, K.H.1    Blitchington, R.B.2
  • 7
    • 0028946084 scopus 로고
    • Phylogenetic identification and in situ detection of individual microbial cells without cultivation
    • Amann R.I., Ludwig W., Schleifer K.H. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 59:1995;143-169.
    • (1995) Microbiol. Rev. , vol.59 , pp. 143-169
    • Amann, R.I.1    Ludwig, W.2    Schleifer, K.H.3
  • 8
    • 0031692488 scopus 로고    scopus 로고
    • Variations of bacterial populations in human feces measured by fluorescent in situ hybridization with group-specific 16S rRNA-targeted oligonucleotide probes
    • Franks A.H., Harmsen H.J., Raangs G.C., Jansen G.J., Schut F., Welling G.W. Variations of bacterial populations in human feces measured by fluorescent in situ hybridization with group-specific 16S rRNA-targeted oligonucleotide probes. Appl. Environ. Microbiol. 64:1998;3336-3345.
    • (1998) Appl. Environ. Microbiol. , vol.64 , pp. 3336-3345
    • Franks, A.H.1    Harmsen, H.J.2    Raangs, G.C.3    Jansen, G.J.4    Schut, F.5    Welling, G.W.6
  • 9
    • 0034017583 scopus 로고    scopus 로고
    • Quantification of bacterial groups within human fecal flora by oligonucleotide probe hybridization
    • Sghir A., Gramet G., Suau A., Rochet V., Pochart P., Doré J. Quantification of bacterial groups within human fecal flora by oligonucleotide probe hybridization. Appl. Environ. Microbiol. 66:2000;2263-2266.
    • (2000) Appl. Environ. Microbiol. , vol.66 , pp. 2263-2266
    • Sghir, A.1    Gramet, G.2    Suau, A.3    Rochet, V.4    Pochart, P.5    Doré, J.6
  • 11
    • 0033781871 scopus 로고    scopus 로고
    • Development of 16S rRNA-based probes for the Coriobacterium group and the Atopobium cluster and their application for enumeration of Coriobacteriaceae in human feces from volunteers of different age groups
    • Harmsen H.J.M., Wildeboer-Veloo A.C.M., Grijpstra J., Knol J., Degener J.E., Welling G.W. Development of 16S rRNA-based probes for the Coriobacterium group and the Atopobium cluster and their application for enumeration of Coriobacteriaceae in human feces from volunteers of different age groups. Appl. Environ. Microbiol. 66:2000;4523-4527.
    • (2000) Appl. Environ. Microbiol. , vol.66 , pp. 4523-4527
    • Harmsen, H.J.M.1    Wildeboer-Veloo, A.C.M.2    Grijpstra, J.3    Knol, J.4    Degener, J.E.5    Welling, G.W.6
  • 13
    • 0031892290 scopus 로고    scopus 로고
    • Design and evaluation of a 16S rRNA-targeted oligonucleotide probe for specific detection and quantitation of human fecal Bacteroides populations
    • Doré J., Sghir A., Hannequart-Gramet G., Corthier G., Pochart P. Design and evaluation of a 16S rRNA-targeted oligonucleotide probe for specific detection and quantitation of human fecal Bacteroides populations. Syst. Appl. Microbiol. 21:1998;65-71.
    • (1998) Syst. Appl. Microbiol. , vol.21 , pp. 65-71
    • Doré, J.1    Sghir, A.2    Hannequart-Gramet, G.3    Corthier, G.4    Pochart, P.5
  • 14
    • 0035652810 scopus 로고    scopus 로고
    • Composition of human intestinal flora analysed by fluorescent in situ hybridisation using group-specific 16S rRNA-targeted oligonucleotide probes
    • Rochet V., Rigottier-Gois L., Beguet F., Doré J. Composition of human intestinal flora analysed by fluorescent in situ hybridisation using group-specific 16S rRNA-targeted oligonucleotide probes. Genet. Sel. Evol. 33:2001;S339-S352.
    • (2001) Genet. Sel. Evol. , vol.33 , pp. 339-S352
    • Rochet, V.1    Rigottier-Gois, L.2    Beguet, F.3    Doré, J.4
  • 15
    • 0024005650 scopus 로고
    • Use of phylogenetically-based hybridization probes for studies of ruminal microbial ecology
    • Stahl D.A., Flesher B., Mansfield H.R., Montgomery L. Use of phylogenetically-based hybridization probes for studies of ruminal microbial ecology. Appl. Environ. Microbiol. 54:1988;1079-1084.
    • (1988) Appl. Environ. Microbiol. , vol.54 , pp. 1079-1084
    • Stahl, D.A.1    Flesher, B.2    Mansfield, H.R.3    Montgomery, L.4
  • 16
    • 0025294528 scopus 로고
    • Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations
    • Amann R.I., Binder B.J., Olson R.J., Chisholm S.W., Devereux R., Stahl D.A. Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl. Environ. Microbiol. 56:1990;1919-1925.
    • (1990) Appl. Environ. Microbiol. , vol.56 , pp. 1919-1925
    • Amann, R.I.1    Binder, B.J.2    Olson, R.J.3    Chisholm, S.W.4    Devereux, R.5    Stahl, D.A.6
  • 17
    • 0027339723 scopus 로고
    • Optimizing fluorescent in situ hybridization with rRNA-targeted oligonucleotide probes for flow cytometric identification of microorganisms
    • Wallner G., Amann R., Beisker W. Optimizing fluorescent in situ hybridization with rRNA-targeted oligonucleotide probes for flow cytometric identification of microorganisms. Cytometry. 14:1993;136-143.
    • (1993) Cytometry , vol.14 , pp. 136-143
    • Wallner, G.1    Amann, R.2    Beisker, W.3
  • 19
    • 0034129592 scopus 로고    scopus 로고
    • Analysis of the fecal microflora of human subjects consuming a probiotic product containing Lactobacillus rhamnosus DR20
    • Tannock G.W., Munro K., Harmsen H.J., Welling G.W., Smart J., Gopal P.K. Analysis of the fecal microflora of human subjects consuming a probiotic product containing Lactobacillus rhamnosus DR20. Appl. Environ. Microbiol. 66:2000;2578-2588.
    • (2000) Appl. Environ. Microbiol. , vol.66 , pp. 2578-2588
    • Tannock, G.W.1    Munro, K.2    Harmsen, H.J.3    Welling, G.W.4    Smart, J.5    Gopal, P.K.6
  • 20
    • 0000191489 scopus 로고
    • (N.R. Krieg, a.J.G.H., Ed.), Williams and Wilkins, Baltimore, MD
    • Scardovi, V. (1984) In: Bergey's Manual of Systematic Bacteriology, Vol. 1, pp. 1418-1434 (N.R. Krieg, a.J.G.H., Ed.), Williams and Wilkins, Baltimore, MD.
    • (1984) Bergey's Manual of Systematic Bacteriology , vol.1 , pp. 1418-1434
    • Scardovi, V.1
  • 21
    • 0030891419 scopus 로고    scopus 로고
    • Administration of transgalacto-oligosaccharides increases fecal bifidobacteria and modifies colonic fermentation metabolism in healthy humans
    • Bouhnik Y., Flourie B., D'Agay-Abensour L., Pochart P., Gramet G., Durand M., Rambaud J.C. Administration of transgalacto-oligosaccharides increases fecal bifidobacteria and modifies colonic fermentation metabolism in healthy humans. J. Nutr. 127:1997;444-448.
    • (1997) J. Nutr. , vol.127 , pp. 444-448
    • Bouhnik, Y.1    Flourie, B.2    D'Agay-Abensour, L.3    Pochart, P.4    Gramet, G.5    Durand, M.6    Rambaud, J.C.7
  • 22
    • 0029817024 scopus 로고    scopus 로고
    • Effects of fructo-oligosaccharides ingestion on fecal bifidobacteria and selected metabolic indexes of colon carcinogenesis in healthy humans
    • Bouhnik Y., Flourie B., Riottot M., Bisetti N., Gailing M.F., Guibert A., Bornet F., Rambaud J.C. Effects of fructo-oligosaccharides ingestion on fecal bifidobacteria and selected metabolic indexes of colon carcinogenesis in healthy humans. Nutr. Cancer. 26:1996;21-29.
    • (1996) Nutr. Cancer , vol.26 , pp. 21-29
    • Bouhnik, Y.1    Flourie, B.2    Riottot, M.3    Bisetti, N.4    Gailing, M.F.5    Guibert, A.6    Bornet, F.7    Rambaud, J.C.8
  • 23
    • 0031789907 scopus 로고    scopus 로고
    • Flow cytometric analysis of the in situ accessibility of Escherichia coli 16S rRNA for fluorescently labeled oligonucleotide probes
    • Fuchs B.M., Wallner G., Beisker W., Schwippl I., Ludwig W., Amann R. Flow cytometric analysis of the in situ accessibility of Escherichia coli 16S rRNA for fluorescently labeled oligonucleotide probes. Appl. Environ. Microbiol. 64:1998;4973-4982.
    • (1998) Appl. Environ. Microbiol. , vol.64 , pp. 4973-4982
    • Fuchs, B.M.1    Wallner, G.2    Beisker, W.3    Schwippl, I.4    Ludwig, W.5    Amann, R.6
  • 24
    • 0035145060 scopus 로고    scopus 로고
    • In situ accessibility of Escherichia coli 23S rRNA to fluorescently labelled oligonucleotide probes
    • Fuchs B.M., Syutsubo K., Ludwig W., Amann R. In situ accessibility of Escherichia coli 23S rRNA to fluorescently labelled oligonucleotide probes. Appl. Environ. Microbiol. 67:2001;961-968.
    • (2001) Appl. Environ. Microbiol. , vol.67 , pp. 961-968
    • Fuchs, B.M.1    Syutsubo, K.2    Ludwig, W.3    Amann, R.4
  • 25
  • 26
    • 0017381987 scopus 로고
    • Fermentation of mucin and plant polysaccharides by strains of Bacteroides from human colon
    • Salyers A.A., Vercellotti J.R., West S.E.H., Wilkins T.D. Fermentation of mucin and plant polysaccharides by strains of Bacteroides from human colon. Appl. Environ. Microbiol. 33:1977;319-322.
    • (1977) Appl. Environ. Microbiol. , vol.33 , pp. 319-322
    • Salyers, A.A.1    Vercellotti, J.R.2    West, S.E.H.3    Wilkins, T.D.4
  • 27
    • 9344247522 scopus 로고    scopus 로고
    • Application of a suite of 16S rRNA-specific oligonucleotide probes designed to investigate bacteria of the phylum cytophaga-flavobacter-bacteroides in the natural environment
    • Manz W., Amann R., Ludwig W., Vancanneyt M., Schleifer K.H. Application of a suite of 16S rRNA-specific oligonucleotide probes designed to investigate bacteria of the phylum cytophaga-flavobacter-bacteroides in the natural environment. Microbiology. 142:1996;1097-1106.
    • (1996) Microbiology , vol.142 , pp. 1097-1106
    • Manz, W.1    Amann, R.2    Ludwig, W.3    Vancanneyt, M.4    Schleifer, K.H.5
  • 29
    • 0029162158 scopus 로고
    • Quantitative fluorescence in situ hybridization of Bifidobacterium spp. with genus-specific 16S rRNA-targeted probes and its application in fecal samples
    • Langendijk P.S., Schut F., Jansen G.J., Raangs G.C., Kamphuis G.R., Wilkinson M.H., Welling G.W. Quantitative fluorescence in situ hybridization of Bifidobacterium spp. with genus-specific 16S rRNA-targeted probes and its application in fecal samples. Appl. Environ. Microbiol. 61:1995;3069-3075.
    • (1995) Appl. Environ. Microbiol. , vol.61 , pp. 3069-3075
    • Langendijk, P.S.1    Schut, F.2    Jansen, G.J.3    Raangs, G.C.4    Kamphuis, G.R.5    Wilkinson, M.H.6    Welling, G.W.7


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