Analysis of the intestinal microflora using molecular methods

European Journal of Clinical Nutrition

Volumn 56, Issue , 2002, Pages S44-S49

  Tannock, G W ^a  

^a UNIVERSITY OF OTAGO   (New Zealand)

Author keywords

Analysis; Atopy; Microflora

Indexed keywords

AMOXICILLIN; ANTIBIOTIC AGENT; ANTIGEN; COTRIMOXAZOLE; FLUCLOXACILLIN; NUCLEIC ACID;

AGE; ANAEROBIC BACTERIUM; ATOPY; BACTERIAL COLONIZATION; BACTERIAL GROWTH; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; BIFIDOBACTERIUM; CHILD; CHILD CARE; CONTROLLED STUDY; DENATURING GRADIENT GEL ELECTROPHORESIS; DRUG USE; FECES COMPOSITION; HUMAN; IMMUNE RESPONSE; INCIDENCE; INTESTINE FLORA; LABORATORY TEST; MEDICAL RESEARCH; MOLECULAR DYNAMICS; NUCLEIC ACID ANALYSIS; POLYMERASE CHAIN REACTION; PREDICTION; REVIEW; SCREENING TEST; TH1 CELL; TH2 CELL; TREATMENT OUTCOME;

ADULT; CHILD; DNA, BACTERIAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FECES; FEMALE; HUMANS; HYPERSENSITIVITY, IMMEDIATE; INTESTINES; MALE; POLYMERASE CHAIN REACTION;

BACTERIA (MICROORGANISMS); BIFIDOBACTERIUM;

EID: 0036972364     PISSN: 09543007     EISSN: 14765640     Source Type: Journal    
DOI: 10.1038/sj.ejcn.1601661     Document Type: Article

References (37)

1. Alm J, Swartz J, Lilja G, Schneyius A and Pershagen G (1999): Atopy in children of families with an anthroposophic lifestyle. Lancet 353, 1485-1488.
2. Beerens H (1990): An elective and selective isolation medium for Bifidobacterium spp. Lett. Appl. Microbiol. 11, 155-157.
3. Biavati B, Castagnoli P, Crociani F and Trovatelli LD (1984): Species of the Bifidobacterium in the feces of infants. Microbiologica 7, 341-345.
4. Biavati B, Castagnoli P and Trovatelli LD (1986): Species of the genus Bifidobacterium in the feces of human adults. Microbiologica 9, 39-45.
5. Bourget N, Simonet J-M and Decaris B (1993): Analysis of the genome of the five Bifidobacterium breve strains: plasmid content, pulsedfield gel electrophoresis genome size estimation and rrn loci number. FEMS Microbiol. Lett. 110, 11-20.
6. Drasar BS and Barrow PA (1985): Intestinal Microbiology. Washington, DC: American Society for Microbiology.
7. Droste J, Wieringa M, Weyler J, Nelen V, Vermiere P and Van Bever H (2000): Does the use of antibiotics in early childhood increase the risk of asthma and allergic disease? Clin. Exp. Allergy 30, 1547-1553.
8. Farooqi I and Hopkin J (1998): Early childhood infection and atopic disorder. Thorax 53, 927-932.
9. Finegold SM and Sutter VL (1978): Fecal flora in different populations, with special reference to diet. Am. J. Clin. Nutr. 31, S116-S122.
10. Finegold SM, Sutter VL and Mathisen GE (1983): Normal indigenous intestinal flora. In: Human Intestinal Microflora in Health and Disease, ed. DJ Hentges, pp 3-31. New York: Academic Press.
11. Franks AH, Harmsen HJ, Raangs GC, Jansen GJ, Schut F and Welling GW (1998): Variations of bacterial populations in human feces measured by fluorescent in situ hybridization with group-specific 16S rRNA-targeted oligonucleotide probes. Appl. Environ. Microbiol. 64, 3336-3345.
12. Frothingham R, Duncan AJ and Wilson KH (1993): Ribosomal DNA sequences of bifidobacteria: implications for sequence-based identification of the human colonic flora. Microb. Ecol. Health Dis. 6, 23-27.
13. Goldin BR and Gorbach SL (1992): Probiotics for humans. In: Probiotics. The Scientific Basis, ed. R Fuller, pp 355-376. London: Chapman and Hall.
14. Harmsen HJM, Wildeboer-Veloo ACM, Raangs GC, Wagendorp AA, Klijn N, Bindels JG and Welling GW (2000): Analysis of intestinal flora development in breast-fed and formula-fed infants by using molecular identification and detection methods. J. Pediatr. Gastroenterol. 30, 61-67.
15. Holdeman LV and Moore WE (1972): Roll-tube techniques for anaerobic bacteria. Am. Clin. Nutr. 25, 1314-1317.
16. Hopkin JM(1997):Mechanisms of enhanced prevalence of asthma and atopy in developed countries. Curr. Opin. Immunol. 9, 788-792.
17. Kimura K, McCartney AL, McConnell MA and Tannock GW (1997): Analysis of fecal populations of bifidobacteria and lactobacilli and investigation of the immunological responses of their human hosts to the predominant strains. Appl. Environ. Microbiol. 63, 3394-3398.
18. Langendijk PS, Schut F, Jansen GJ, Raangs GC, Kamphuis GR, Wilkinson MH and Welling GW (1995): Quantitative fluorescence in situ hybridisation of Bifidobacterium spp. with genus-specific 16S rRNA-targeted probes and its application in fecal samples. Appl. Environ. Microbiol. 61, 3069-3075.
19. Matsuki T, Watanabe K, Tanaka R, Fukuda M and Oyaizu H (1999): Distribution of bifidobacterial species in human intestinal microflora examined with 16S rRNA-gene targeted species-specific primers. Appl. Environ. Microbiol. 65, 4506-4512.
20. Mitsuoka T (1992): The human gastrointestinal tract. In: The Lactic Acid Bacteria, Vol. 1, ed. BJB Wood, pp 69-114. London: Elsevier Applied Science.
21. Moore WE and Holdeman LV (1974): Special problems associated with the isolation and identification of intestinal bacteria in fecal flora studies. Am. J. Clin. Nutr. 27, 1450-1455.
22. Moore WE, Cato EP and Holdeman LV (1978): Some current concepts in intestinal bacteriology. Am. J. Clin. Nutr. 31, S33-S42.
23. Muyzer G and Smalla K (1998): Application of denaturing gradient gel electrophoresis (DGGE)andtemperature gradient gel electrophoresis (TGGE) in microbial ecology. Antonie Van Leeuwenhoek 73, 127-141.
24. O'Sullivan DJ (1999): Methods of analysis of the intestinal microflora. In: Probiotics: a Critical Review, ed. GW Tannock, pp 23-44.Wymondham: Horizon Scientific Press.
25. Ouwehand AC, Isolauri E, He F, Hashimoto H, Benno Y, Salminen S (2001): Differences in Bifidobacterium flora composition in allergic and healthy infants. J. Allergy Clin. Immunol. 108, 144-145.
26. Satokari RM, Vaughan EE, Akkermans ADL, Saarela M and De Vos WM (2001): Bifidobacterial diversity in human feces detected by genusspecific PCR and denaturing gradient gel electrophoresis. Appl. Environ. Microbiol. 67, 504-513.
27. Sghir A, Gramet G, Suau A, Rochet V, Pochart P and Dore J (2000): Quantification of bacterial groups within the human fecal flora by oligonucleotide probe hybridization. Appl. Environ. Microbiol. 66, 2263-2266.
28. Sgorbati B, Biavati B and Palenzona D (1995): The genus Bifidobacterium. In: The Lactic Acid Bacteria, Vol. 2. The Genera of Lactic Acid Bacteria, ed. BJB Wood and Holzapfel WH, pp 279-306. London: Blackie Academic and Professional.
29. Suau A, Bonnet R, Sutren M, Godon J-J, Gibson GR, Collins MD and Dore J (1999): Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut. Appl. Environ. Microbiol. 65, 4799-4807.
30. Tannock GW (1999): Identification of lactobacilli and bifidobacteria. In: Probiotics: a Critical Review, ed. GW Tannock, pp 45-56. Wymondham: Horizon Scientific Press.
31. Tannock GW, Munro K, Harmsen HJM, Welling GW, Smart J and Gopal PK (2000): Analysis of the fecal microflora of human subjects consuming a probiotic product containing Lactobacillus rhamnosus DR20. Appl. Environ. Microbiol. 66, 2578-2588.
32. Vaughan EE, Schut F, Heilig HGHJ, Zoetendal EG, De Vos WM and Akkermans ADL (2000): A molecular view of the intestinal ecosystem. Curr. Issues Intest. Microbiol. 1, 1-12.
33. Walter J, Hertel C, Tannock GW, Lis CM, Munro K and Hammes WP (2001): Detection of Lactobacillus, Pediococcus, Leuconostoc, and Weisella species in human feces by using group-specific PCR primers and denaturing gradient gel electrophoresis. Appl. Environ. Microbiol. 67, 2578-2585.
34. Welling GW, Elfferich P, Raangs GC, Wildeboer-Veloo AC, Jansen GJ, Degener JE (1997): 16S Ribosomal RNA-targeted oligonucleotide probes for monitoring of intestinal tract bacteria. Scand. J. Gastroenterol. 222, 17-19.
35. Wickens K, Pearce N, Crane J and Beasley R (1999): Antibiotic use in early childhood and the development of asthma. Clin. Exp. Allergy 29, 766-771.
36. Woese CR (1987): Bacterial evolution. Microbiol. Rev. 51, 221-271.
37. Zoetendal EG, Akkermans AD and De Vos WM (1998): Temperature gradient gel electrophoresis analysis of 16S rRNA from human fecal samples reveals stable and host-specific communities of active bacteria. Appl. Environ. Microbiol. 64, 3854-3859.