Gene-specific random mutagenesis of Escherichia coli in vivo: Isolation of temperature-sensitive mutations in the acyl carrier protein of fatty acid synthesis

Journal of Bacteriology

Volumn 188, Issue 1, 2006, Pages 287-296

  De Lay, Nicholas R ^a   Cronan, John E ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACYL CARRIER PROTEIN; FATTY ACID; MUTANT PROTEIN; PANTETHEINE PHOSPHATE; ACPP PROTEIN, E COLI; APOPROTEIN; ESCHERICHIA COLI PROTEIN;

ACPP GENE; ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIOPHAGE LAMBDA; CONTROLLED STUDY; ESCHERICHIA COLI; FATTY ACID SYNTHESIS; GENE ISOLATION; GENE MUTATION; HOMOLOGOUS RECOMBINATION; LIPOGENESIS; MUTAGENESIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN LIPID INTERACTION; PROTEIN STABILITY; STRUCTURAL GENE; TEMPERATURE SENSITIVE MUTANT; WILD TYPE; AMINO ACID SUBSTITUTION; BIOSYNTHESIS; CHEMICAL STRUCTURE; ENZYMOLOGY; ESCHERICHIA COLI K 12; ESSENTIAL GENE; EVALUATION; GENETIC PROCEDURES; GENETIC RECOMBINATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; TEMPERATURE;

BACTERIA (MICROORGANISMS); ENTEROBACTERIA PHAGE LAMBDA; ESCHERICHIA COLI;

ACYL CARRIER PROTEIN; AMINO ACID SUBSTITUTION; APOPROTEINS; ESCHERICHIA COLI K12; ESCHERICHIA COLI PROTEINS; FATTY ACIDS; GENES, ESSENTIAL; GENETIC TECHNIQUES; MODELS, MOLECULAR; MUTAGENESIS; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC; TEMPERATURE;

EID: 33644907784     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.188.1.287-296.2006     Document Type: Article

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